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Gene Expression Data
Assay Details
Assay
Reference: J:324823 Mitchel K, et al., Hoxa5 Activity Across the Lateral Somitic Frontier Regulates Development of the Mouse Sternum. Front Cell Dev Biol. 2022;10:806545
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7309991
Gene symbol: Sox9
Gene name: SRY (sex determining region Y)-box 9
Antibody: Anti-Sox9 (AB5535)
Detection system: Secondary antibody
Results
Specimen 6A/J: embryonic day 13.5; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Meox1tm1(cre)Jpa/Meox1+ (more )
Note: Conditional mutant. Interclavicular mesenchyme. Triple labeled: blue - Sox9; green - Hoxa5 (assay MGI:7309976); red - Cre reporter.
Structure Level Pattern Image Note
TS21: clavicle pre-cartilage condensation Present 6A' , 6J , 6A"' , 6A
TS21: pectoral-thoracic pre-cartilage condensation Present Regionally restricted 6J , 6A' , 6A"' , 6A Expressed in the presternum, which contained: paired dorsal elements that contact the clavicles at a lower level; separate paired elements that lie ventral to the clavicle, and a ventral-midline element where pectoral muscle attachment occurs.
Specimen 6B/K: embryonic day 13.5; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Meox1tm1(cre)Jpa/Meox1+ (more )
Note: Conditional mutant. Interclavicular mesenchyme. Triple labeled: blue - Sox9; green - Hoxa5 (assay MGI:7309976); red - Cre reporter.
Structure Level Pattern Image Note
TS21: clavicle pre-cartilage condensation Present 6B , 6K , 6B"' , 6B'
TS21: pectoral-thoracic pre-cartilage condensation Present Regionally restricted 6B"' , 6K , 6B , 6B' Expressed in the presternum, which contained: paired dorsal elements that contact the clavicles at a lower level; separate paired elements that lie ventral to the clavicle, and a ventral-midline element where pectoral muscle attachment occurs.
Specimen 6C/L: embryonic day 13.5; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Meox1tm1(cre)Jpa/Meox1+ (more )
Note: Conditional mutant. Intercostoclavicular mesenchyme. Triple labeled: blue - Sox9; green - Hoxa5 (assay MGI:7309976); red - Cre reporter.
Structure Level Pattern Image Note
TS21: pectoral-thoracic pre-cartilage condensation Present Regionally restricted 6C' , 6C"' , 6C , 6L Expressed in the presternum. The ventral paired elements were larger and bar-shaped with increased expression and incomplete fusion.
Specimen 6D/M: embryonic day 13.5; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Meox1tm1(cre)Jpa/Meox1+ (more )
Note: Conditional mutant. Intercostoclavicular mesenchyme. Triple labeled: blue - Sox9; green - Hoxa5 (assay MGI:7309976); red - Cre reporter.
Structure Level Pattern Image Note
TS21: pectoral-thoracic pre-cartilage condensation Present Regionally restricted 6D"' , 6D , 6M , 6D' Expressed in the presternum. The ventral paired elements were larger and bar-shaped with increased expression and incomplete fusion.
Specimen 6E/N: embryonic day 13.5; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Meox1tm1(cre)Jpa/Meox1+ (more )
Note: Conditional mutant. Intercostoclavicular mesenchyme. Triple labeled: blue - Sox9; green - Hoxa5 (assay MGI:7309976); red - Cre reporter.
Structure Level Pattern Image Note
TS21: pectoral-thoracic pre-cartilage condensation Present Regionally restricted 6E' , 6E"' , 6N , 6E Expressed in the presternum. The ventral paired elements were larger and bar-shaped with increased expression and incomplete fusion.
Specimen 6F/O: embryonic day 13.5; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Meox1tm1(cre)Jpa/Meox1+ (more )
Note: Conditional mutant. Rib 1. Triple labeled: blue - Sox9; green - Hoxa5 (assay MGI:7309976); red - Cre reporter.
Structure Level Pattern Image Note
TS21: T1 rib cartilage condensation Present 6F' , 6F , 6F"' , 6O
TS21: pectoral-thoracic pre-cartilage condensation Present Regionally restricted 6F , 6F"' , 6F' , 6O Expressed in the presternum. Rib 1 appeared to make contact with the rounded, medial region.
Specimen 6G/P: embryonic day 13.5; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Meox1tm1(cre)Jpa/Meox1+ (more )
Note: Conditional mutant. Rib 1. Triple labeled: blue - Sox9; green - Hoxa5 (assay MGI:7309976); red - Cre reporter.
Structure Level Pattern Image Note
TS21: T1 rib cartilage condensation Present 6P , 6G' , 6G , 6G"'
TS21: pectoral-thoracic pre-cartilage condensation Present Regionally restricted 6G"' , 6G , 6P , 6G' Expressed in the presternum. Rib 1 appeared to make contact with the rounded, medial region. Sternal bar fusion was also incomplete.
Specimen 6H/Q: embryonic day 13.5; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Meox1tm1(cre)Jpa/Meox1+ (more )
Note: Conditional mutant. Rib 1. Triple labeled: blue - Sox9; green - Hoxa5 (assay MGI:7309976); red - Cre reporter.
Structure Level Pattern Image Note
TS21: T1 rib cartilage condensation Present 6H , 6H' , 6Q , 6H"'
TS21: pectoral-thoracic pre-cartilage condensation Present Regionally restricted 6Q , 6H' , 6H"' , 6H Expressed in the presternum. Rib 1 appeared to make contact with the rounded, medial region. Sternal bar fusion was also incomplete, with a loose collection of Sox9-expressing cells extending between them.
Specimen 6I/R: embryonic day 13.5; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Meox1tm1(cre)Jpa/Meox1+ (more )
Note: Conditional mutant. Rib 2/3. Triple labeled: blue - Sox9; green - Hoxa5 (assay MGI:7309976); red - Cre reporter.
Structure Level Pattern Image Note
TS21: T1 rib cartilage condensation Present 6I , 6I"' , 6R , 6I'
TS21: T2 rib cartilage condensation Present 6I , 6I"' , 6R , 6I'
TS21: pectoral-thoracic pre-cartilage condensation Present Regionally restricted 6R , 6I"' , 6I' , 6I Expressed in the presternum. Paired, unfused sternal bars were seen at the point of rib attachment, connected by a stream of Sox9-positive cells.
Specimen 8O: embryonic day 11.5; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Tg(Prrx1-cre)1Cjt/0 (more )
Note: Conditional mutant. Double labeled: cyan - Sox9; red - Cre reporter.
Structure Level Pattern Image Note
TS19: trunk mesenchyme Weak Regionally restricted 8O inset , 8O Expressed in a subdomain of Hoxa5-positive cells.
Specimen 8S: embryonic day 11.5; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Tg(Prrx1-cre)1Cjt/0 (more )
Note: Conditional mutant. Double labeled: cyan - Sox9; red - Cre reporter.
Structure Level Pattern Image Note
TS19: trunk mesenchyme Present Regionally restricted 8S Expressed ventral to the forelimb, a region previously shown to contain sternal bar progenitors.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
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