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Gene Expression Data
Assay Details
Assay
Reference: J:257920 Tahara N, et al., Gata6 restricts Isl1 to the posterior of nascent hindlimb buds through Isl1 cis-regulatory modules. Dev Biol. 2018 Feb 1;434(1):74-83
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7256725
Gene symbol: Isl1
Gene name: ISL1 transcription factor, LIM/homeodomain
Antibody: clone 39.4D5
Detection system: Secondary antibody
Results
Specimen 6C/D: embryonic day 9.5 (more )
Note: 28 somite stage. Triple labeled: green - Isl1; magenta - Gata6 (assay MGI:7256724); blue - DAPI.
Structure Level Pattern Image Note
TS15: hindlimb ridge Present Regionally restricted 6C , 6D High expression in posterior; sharp decline in in expression from middle to anterior of hindlimb bud.
Specimen 6G/H: embryonic day 9.5; Gata6tm2.1Sad/Gata6tm2.1Sad, Tg(T-cre)1Lwd/0 (more )
Note: Conditional mutant. 28 somite stage. Triple labeled: green - Isl1; magenta - Gata6 (assay MGI:7256724); blue - DAPI.
Structure Level Pattern Image Note
TS15: hindlimb ridge Strong Regionally restricted 6H , 6G Increase in expression intensity compared to wild type; region of highest expression shifted anteriorly.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory