Dmd Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:226931 Blondelle J, et al., HACD1, a regulator of membrane composition and fluidity, promotes myoblast fusion and skeletal muscle growth. J Mol Cell Biol. 2015 Oct;7(5):429-40
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6790381
Gene symbol:	Dmd
Gene name:	dystrophin, muscular dystrophy

Antibody:	anti-Dystrophin
Detection system:	Secondary antibody coupled to Alexa Fluor 488
Assay notes:	Fixative was acetone/methanol.

Results

Specimen S3A: postnatal; Hacd1^tm1.1Pas/Hacd1⁺ (more

)
Note: Control mice were either heterozygotes or +/+. Double labeled: green - Dmd; red - Pax7 (assay MGI:6790379).

Structure	Level	Pattern	Image	Note
TS28: tibialis anterior	Present		S3A

Specimen +/+: postnatal (more

)

Structure	Level	Pattern	Image	Note
TS28: tibialis anterior	Present

Specimen S3B: postnatal; Hacd1^tm1.1Pas/Hacd1^tm1.1Pas (more

)
Note: Double labeled: green - Dmd; red - Pax7 (assay MGI:6790379).

Structure	Level	Pattern	Image	Note
TS28: tibialis anterior	Present		S3B	Expression in this mutant is similar to control mice.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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