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Gene Expression Data
Assay Details
Assay
Reference: J:256775 Zhang H, et al., Endocardium Minimally Contributes to Coronary Endothelium in the Embryonic Ventricular Free Walls. Circ Res. 2016 Jun 10;118(12):1880-93
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6723818
Gene symbol: Cdh5
Gene name: cadherin 5
Antibody: anti-VE-cadherin (AF1002)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 1D: embryonic day 9.5; Nfatc1tm2(cre)Bzsh/Nfatc1+, Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Double labeled: green - Cdh5; red - Cre reporter.
Structure Level Pattern Image Note
TS15: atrium endocardial lining Present 1D inset , 1D merge , 1D green Expression is seen in endothelial cells.
TS15: primitive ventricle endocardial lining Present 1D inset , 1D merge , 1D green Expression is seen in endothelial cells.
TS15: sinus venosus Present Regionally restricted 1D green , 1D merge , 1D inset Expression is seen in endothelial cells.
TS15: heart endothelium Present 1D merge , 1D inset , 1D green Expression is seen in endothelial cells.
Specimen 1E: embryonic day 11.5; Nfatc1tm2(cre)Bzsh/Nfatc1+, Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Double labeled: green - Cdh5; red - Cre reporter.
Structure Level Pattern Image Note
TS19: ventricle endocardial lining Present 1E merge , 1E green Expression is seen in endothelial cells.
TS19: atrium endocardial lining Present 1E merge , 1E green , 1E inset Expression is seen in endothelial cells.
TS19: sinus venosus Present Regionally restricted 1E green , 1E inset , 1E merge Expression is seen in endothelial cells.
TS19: heart endothelium Present 1E inset , 1E green , 1E merge Expression is seen in endothelial cells.
Specimen 5A: embryonic day 9.5; Npr3em1(cre/ERT2)Bzsh/Npr3+ (more )
Note: Double labeled: green - Cdh5; red - Npr3 reporter (assay MGI:6724010).
Structure Level Pattern Image Note
TS15: primitive ventricle endocardial lining Present 5A green , 5A1 , 5A merge Expression is seen in endothelial cells.
TS15: common atrial chamber endocardial lining Present 5A merge , 5A green , 5A2 Expression is seen in endothelial cells.
TS15: sinus venosus Present Regionally restricted 5A2 , 5A green , 5A merge Expression is seen in endothelial cells.
TS15: dorsal aorta Present Regionally restricted 5A green , 5A merge Expression is seen in endothelial cells.
Specimen 5B: embryonic day 10.5; Npr3em1(cre/ERT2)Bzsh/Npr3+ (more )
Note: Double labeled: green - Cdh5; red - Npr3 reporter (assay MGI:6724010).
Structure Level Pattern Image Note
TS17: primitive ventricle endocardial lining Present 5B1 , 5B merge , 5B green Expression is seen in endothelial cells.
TS17: common atrial chamber endocardial lining Present 5B merge , 5B green , 5B2 Expression is seen in endothelial cells.
TS17: sinus venosus Present Regionally restricted 5B2 , 5B green , 5B merge Expression is seen in endothelial cells.
TS17: dorsal aorta Present Regionally restricted 5B green , 5B merge Expression is seen in endothelial cells.
Specimen 5C: embryonic day 11.5; Npr3em1(cre/ERT2)Bzsh/Npr3+ (more )
Note: Double labeled: green - Cdh5; red - Npr3 reporter (assay MGI:6724010).
Structure Level Pattern Image Note
TS19: ventricle endocardial lining Present 5C merge , 5C green , 5C1 Expression is seen in endothelial cells.
TS19: atrium endocardial lining Present 5C2 , 5C merge , 5C green Expression is seen in endothelial cells.
TS19: sinus venosus Present Regionally restricted 5C green , 5C2 , 5C merge Expression is seen in endothelial cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory