Ascl1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:294646 Stupnikov MR, et al., Jagged and Delta-like ligands control distinct events during airway progenitor cell differentiation. Elife. 2019 Oct 21;8:e50487
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6695189
Gene symbol:	Ascl1
Gene name:	achaete-scute family bHLH transcription factor 1

Antibody:	anti-MASH (24B72D11), eBioscience
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Results

4A left ©

Specimen 4A control: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: lobar bronchus epithelium	Present	Clusters	4A left	Expressed in epithelial cell clusters at sites of neuroepithelial bodies formation.

4A right ©

Specimen 4A Dll1/4 cnull: embryonic day 14.5; Dll1^tm1Mjo/Dll1^tm1Mjo, Dll4^tm1Frad/Dll4^tm1Frad, Shh^{tm1(EGFP/cre)Cjt}/Shh⁺ (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS22: lobar bronchus epithelium	Present		4A right	A striking expansion in the population of positive cells compared with the control.

Specimen 4B control: embryonic day 18.5 (more

)
Note: Double labeled: green - Ascl1; red - Calca (assay MGI:6695174).

Structure	Level	Pattern	Image	Note
TS26: lobar bronchus epithelium	Present	Clusters	4B left lower , 4B left upper	Expressed in epithelial cell clusters at sites of neuroepithelial bodies formation.

Specimen 4B Dll1/4 cnull: embryonic day 18.5; Dll1^tm1Mjo/Dll1^tm1Mjo, Dll4^tm1Frad/Dll4^tm1Frad, Shh^{tm1(EGFP/cre)Cjt}/Shh⁺ (more

)
Note: Conditional mutant. Double labeled: green - Ascl1; red - Calca (assay MGI:6695174).

Structure	Level	Pattern	Image	Note
TS26: lobar bronchus epithelium	Present		4B right upper , 4B right lower	A striking expansion in the population of positive cells compared with the control.

Specimen 4C control: embryonic day 18.5 (more

)
Note: Double labeled: green - Ascl1; red - Mki67 (assay MGI:6695190).

Structure	Level	Pattern	Image	Note
TS26: lobar bronchus epithelium	Present	Clusters	4C upper left	Expressed in epithelial cell clusters at sites of neuroepithelial bodies formation.

Specimen 4C Dll1/4 cnull: embryonic day 18.5; Dll1^tm1Mjo/Dll1^tm1Mjo, Dll4^tm1Frad/Dll4^tm1Frad, Shh^{tm1(EGFP/cre)Cjt}/Shh⁺ (more

)
Note: Conditional mutant. Double labeled: green - Ascl1; red - Mki67 (assay MGI:6695190).

Structure	Level	Pattern	Image	Note
TS26: lobar bronchus epithelium	Present		4C upper right	A striking expansion in the population of positive cells compared with the control.

Specimen 4C Dll1/4 cnull-2: embryonic day 14.5; Dll1^tm1Mjo/Dll1^tm1Mjo, Dll4^tm1Frad/Dll4^tm1Frad, Shh^{tm1(EGFP/cre)Cjt}/Shh⁺ (more

)
Note: Conditional mutant. Double labeled: green - Ascl1; red - Mki67 (assay MGI:6695190).

Structure	Level	Pattern	Image	Note
TS22: lobar bronchus epithelium	Present		4C lower upper , 4C lower lower left	A striking expansion in the population of positive cells compared with the control.

Specimen 5A control: embryonic day 18.5 (more

)
Note: Double labeled: green - Ascl1; red - Notch1 (assay MGI:6695196).

Structure	Level	Pattern	Image	Note
TS26: lobar bronchus epithelium	Present	Clusters	5A control upper	Expressed in epithelial cell clusters at sites of neuroepithelial bodies formation.

5A cKO upper ©

Specimen 5A Dll1/4 cnull: embryonic day 18.5; Dll1^tm1Mjo/Dll1^tm1Mjo, Dll4^tm1Frad/Dll4^tm1Frad, Shh^{tm1(EGFP/cre)Cjt}/Shh⁺ (more

)
Note: Conditional mutant. Double labeled: green - Ascl1; red - Notch1 (assay MGI:6695196).

Structure	Level	Pattern	Image	Note
TS26: lobar bronchus epithelium	Present	Clusters	5A cKO upper	An expansion in the population of positive cells compared with the control.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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