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Gene Expression Data
Assay Details
Assay
Reference: J:299155 Miao X, et al., Loss of POLR1D results in embryonic lethality prior to blastocyst formation in mice. Mol Reprod Dev. 2020 Nov;87(11):1152-1158
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6515712
Gene symbol: Pou5f1
Gene name: POU domain, class 5, transcription factor 1
Antibody: anti-Oct4 (ab27985)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 3 Polr1d+/+: embryonic day 3.0 (more )
Note: Triple labeled: green - Pou5f1; white - Cdx2 (assay MGI:6515713); red - Trp53 (assay MGI:6515710).
Structure Level Pattern Image Note
TS4: compacted morula Present 3_Merged Polr1d+/+ , 3_OCT4 Polr1d+/+
Specimen 3 Polr1d+/-: embryonic day 3.0; Polr1dem1(IMPC)J/Polr1d+ (more )
Note: Triple labeled: green - Pou5f1; white - Cdx2 (assay MGI:6515713); red - Trp53 (assay MGI:6515710).
Structure Level Pattern Image Note
TS4: compacted morula Present 3_OCT4 Polr1d+/- , 3_Merged Polr1d+/-
Specimen 3 Polr1d-/-: embryonic day 3.0; Polr1dem1(IMPC)J/Polr1dem1(IMPC)J (more )
Note: Triple labeled: green - Pou5f1; white - Cdx2 (assay MGI:6515713); red - Trp53 (assay MGI:6515710).
Structure Level Pattern Image Note
TS4: compacted morula Present 3_OCT4 Polr1d-/- , 3_Merged Polr1d-/- Expression in this mutant is similar to wild-type embryos.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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