Gene Expression Data
Assay Details
Assay
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Reference:
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J:127471
Wang S, et al., Loss of Myt1 function partially compromises endocrine islet cell differentiation and pancreatic physiological function in the mouse. Mech Dev. 2007 Nov-Dec;124(11-12):898-910
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6449811
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Gene symbol:
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Sst
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Gene name:
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somatostatin
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Antibody:
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anti-SS
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Assay notes:
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Two antibodies were described, but the one used in this assay was not detailed. Anti-SS MGI:6449779 may have been used in this assay.
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Results
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Specimen
1D1:
embryonic day 15.5
(more )
Note: Double labeled: green - Sst; red - Myt1 (assay MGI:6449800).
Specimen
1D1:
(close )
Genetic Background:
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involves: 129 * C57BL/6 * CBA * CD-1 * SJL
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - Sst; red - Myt1 (assay MGI:6449800).
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Specimen
1D2:
postnatal week 12
(more )
Note: Double labeled: green - Sst; red - Myt1 (assay MGI:6449800).
Specimen
1D2:
(close )
Genetic Background:
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involves: 129 * C57BL/6 * CBA * CD-1 * SJL
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Age:
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postnatal week 12
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - Sst; red - Myt1 (assay MGI:6449800).
|
Specimen
1D2 inset:
postnatal week 12
(more )
Note: Double labeled: green - Sst; red - Myt1 (assay MGI:6449800).
Specimen
1D2 inset:
(close )
Genetic Background:
|
involves: 129 * C57BL/6 * CBA * CD-1 * SJL
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Age:
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postnatal week 12
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - Sst; red - Myt1 (assay MGI:6449800).
|
Specimen
5B1:
embryonic day 15.5
(more )
Note: Double labeled: red - Sst; green - Ppy (assay MGI:6449810).
Specimen
5B1:
(close )
Genetic Background:
|
involves: 129 * C57BL/6 * CBA * CD-1 * SJL
|
Age:
|
embryonic day 15.5
|
Sex:
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Not Specified
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Type:
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optical section
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Fixation:
|
4% Paraformaldehyde
|
Note:
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Double labeled: red - Sst; green - Ppy (assay MGI:6449810).
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: islets of Langerhans |
Present |
Regionally restricted |
5B1
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Substantial number of cells co-express Sst and Ppy.
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Specimen
5B2:
embryonic day 15.5; Myt1tm1.1Ggu/Myt1 +
(more )
Note: Double labeled: red - Sst; green - Ppy (assay MGI:6449810).
Specimen
5B2:
(close )
Genetic Background:
|
involves: 129 * C57BL/6 * CBA * CD-1 * SJL
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Mutant Allele(s):
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Myt1tm1.1Ggu/Myt1+
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
|
optical section
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Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: red - Sst; green - Ppy (assay MGI:6449810).
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: islets of Langerhans |
Present |
Regionally restricted |
5B2
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Substantial number of cells co-express Sst and Ppy.
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Specimen
5B3:
(close )
Genetic Background:
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involves: 129 * C57BL/6 * CBA * CD-1 * SJL
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Mutant Allele(s):
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Myt1tm1.1Ggu/Myt1tm1.1Ggu
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: red - Sst; green - Ppy (assay MGI:6449810).
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: islets of Langerhans |
Present |
Regionally restricted |
5B3
|
Substantial number of cells co-express Sst and Ppy.
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Specimen
5D1:
embryonic day 18.5
(more )
Note: Double labeled: red - Sst; green - Ppy (assay MGI:6449810).
Specimen
5D1:
(close )
Genetic Background:
|
involves: 129 * C57BL/6 * CBA * CD-1 * SJL
|
Age:
|
embryonic day 18.5
|
Sex:
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Not Specified
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Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: red - Sst; green - Ppy (assay MGI:6449810).
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: islets of Langerhans |
Present |
Regionally restricted |
5D1
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Substantial number of cells co-express Sst and Ppy.
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Specimen
5D2:
embryonic day 18.5; Myt1tm1.1Ggu/Myt1 +
(more )
Note: Double labeled: red - Sst; green - Ppy (assay MGI:6449810).
Specimen
5D2:
(close )
Genetic Background:
|
involves: 129 * C57BL/6 * CBA * CD-1 * SJL
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Mutant Allele(s):
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Myt1tm1.1Ggu/Myt1+
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Age:
|
embryonic day 18.5
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Sex:
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Not Specified
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Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: red - Sst; green - Ppy (assay MGI:6449810).
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: islets of Langerhans |
Present |
Regionally restricted |
5D2
|
Substantial number of cells co-express Sst and Ppy.
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Specimen
5D3:
(close )
Genetic Background:
|
involves: 129 * C57BL/6 * CBA * CD-1 * SJL
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Mutant Allele(s):
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Myt1tm1.1Ggu/Myt1tm1.1Ggu
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Age:
|
embryonic day 18.5
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Sex:
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Not Specified
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Type:
|
optical section
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Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: red - Sst; green - Ppy (assay MGI:6449810).
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: islets of Langerhans |
Present |
Regionally restricted |
5D3
|
Substantial number of cells co-express Sst and Ppy.
|
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