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Gene Expression Data
Assay Details
Assay
Reference: J:278346 Winkler CC, et al., Loss of Shh signaling in the neocortex reveals heterogeneous cell recovery responses from distinct oligodendrocyte populations. Dev Biol. 2019 Aug 1;452(1):55-65
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6448406
Gene symbol: Olig2
Gene name: oligodendrocyte transcription factor 2
Antibody: anti-Olig2
Detection system: Secondary antibody
Results
Specimen 1B +/+: embryonic day 17.5; Emx1tm1(cre)Krj/Emx1+, Gt(ROSA)26Sortm1(CAG-lacZ,-EGFP)Glh/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Vibratome section.
Structure Level Pattern Image Note
TS25: neocortex region Present Regionally restricted 1B Smo +/+ Expression is in oligodendrocytes in the somatosensory cortex.
Specimen 1B fl/fl: embryonic day 17.5; Emx1tm1(cre)Krj/Emx1+, Gt(ROSA)26Sortm1(CAG-lacZ,-EGFP)Glh/Gt(ROSA)26Sor+, Smotm2Amc/Smotm2Amc (more )
Note: Conditional mutant. Vibratome section.
Structure Level Pattern Image Note
TS25: neocortex region Present Single cells 1B Smofl/fl The number of positive oligodendrocytes in the somatosensory cortex is significantly reduced in this mutant compared to controls.
Specimen 1E +/+: postnatal day 30; Emx1tm1(cre)Krj/Emx1+, Gt(ROSA)26Sortm1(CAG-lacZ,-EGFP)Glh/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Vibratome section.
Structure Level Pattern Image Note
TS28: somatosensory cortex Present 1E Smo+/+
Specimen 1E fl/fl: postnatal day 30; Emx1tm1(cre)Krj/Emx1+, Gt(ROSA)26Sortm1(CAG-lacZ,-EGFP)Glh/Gt(ROSA)26Sor+, Smotm2Amc/Smotm2Amc (more )
Note: Conditional mutant. Vibratome section.
Structure Level Pattern Image Note
TS28: somatosensory cortex Present 1E Smofl/fl Expression is about 75% of that seen in controls.
Specimen 5B Gray: postnatal day 30; Emx1tm1(cre)Krj/Emx1+, Gt(ROSA)26Sortm1(CAG-lacZ,-EGFP)Glh/Gt(ROSA)26Sor+, Smotm2Amc/Smotm2Amc (more )
Note: Conditional mutant. Triple labeled: green - Olig2; red - Apc (assay MGI:6448419); blue - Pdgfra (assay MGI:6448421).
Structure Level Pattern Image Note
TS28: brain gray matter Present Single cells 5B Gray Merge , 5B Gray Olig2 Expression is in oligodendorcytes.
Specimen 5B White: postnatal day 30; Emx1tm1(cre)Krj/Emx1+, Gt(ROSA)26Sortm1(CAG-lacZ,-EGFP)Glh/Gt(ROSA)26Sor+, Smotm2Amc/Smotm2Amc (more )
Note: Conditional mutant. Triple labeled: green - Olig2; red - Apc (assay MGI:6448419); blue - Pdgfra (assay MGI:6448421).
Structure Level Pattern Image Note
TS28: cerebral hemisphere white matter Present Single cells 5B White Olig2 , 5B White Merge Expression is in oligodendorcytes.
Specimen 7A Smo+/+: postnatal day 4; Emx1tm1(cre)Krj/Emx1+, Gt(ROSA)26Sortm1(CAG-lacZ,-EGFP)Glh/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Triple labeled: blue - Olig2; green - Mki67 (assay MGI:6448422).
Structure Level Pattern Image Note
TS28: neocortex Present Single cells 7A Smo+/+ Merge , 7A Smo+/+ Olig2
Specimen 7A Smofl/fl: postnatal day 4; Emx1tm1(cre)Krj/Emx1+, Gt(ROSA)26Sortm1(CAG-lacZ,-EGFP)Glh/Gt(ROSA)26Sor+, Smotm2Amc/Smotm2Amc (more )
Note: Conditional mutant. Triple labeled: blue - Olig2; green - Mki67 (assay MGI:6448422).
Structure Level Pattern Image Note
TS28: neocortex Present Single cells 7A Smofl/fl Merge , 7A Smofl/fl Olig2
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
MGI 6.23 		The Jackson Laboratory