Gt(ROSA)26Sor^{tm1(CAG-lacZ,-EGFP)Glh}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-lacZ,-EGFP)Glh}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, David J Goldhamer
• MGI ID: MGI:3840211
• Synonyms: R26^NZG, R-GFP, Rosa26-CAG-loxP-STOP-loxP
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-lacZ,-EGFP)Glh} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:147304
• Parent Cell Line: D1 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6J)F1

Mutation description

Allele Type: Targeted (Conditional ready, Reporter) Mutation: Insertion Mutation details: A vector containing a CAG promoter driving expression of a floxed neo/STOP cassette, frt flanked lacZ gene with 3 polyadenylation sequences (secondary STOP cassette), and an EGFP gene was inserted into the locus. Expression of lacZ requires cre mediated removal of the primary STOP cassette whereas expression of EGFP requires cre mediated removal of the primary stop cassette and flp mediated removal of the secondary STOP cassette. (J:147304)

Generation of Gt(ROSA)26Sor^tm1(CAG-lacZ,-EGFP)Glh, Gt(ROSA)26Sor^tm1.1(CAG-lacZ,-EGFP)Glh, Gt(ROSA)26Sor^tm1.2(CAG-EGFP)Glh, and Gt(ROSA)26Sor^tm1.3(CAG-EGFP)Glh alleles

Expression

In Mice Carrying this Mutation:

43 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 942 strains or lines available

References

• Original: J:147304 Yamamoto M, et al., A multifunctional reporter mouse line for Cre- and FLP-dependent lineage analysis. Genesis. 2009 Feb;47(2):107-14
• All: 38 reference(s)