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Gene Expression Data
Assay Details
Assay
Reference: J:277394 Tellier AP, et al., The elongation factor Elof1 is required for mammalian gastrulation. PLoS One. 2019;14(7):e0219410
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6446226
Gene symbol: Pou5f1
Gene name: POU domain, class 5, transcription factor 1
Antibody: anti-Oct3/4 (C-20; sc-8629)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Assay notes: The sections used in this experiment were subjected to two rounds of multi-color immunofluorence as well as hematoxylin and eosin staining to reveal morphology. Red staining is Cdh1 (assay MGI:6446224); green is either Pou5f1 (Oct4) (assay MGI:6446226) or T (assay MGI:6446227); sections were also stained for phosphorylated histone (white) and DAPI (blue).
Results
Specimen 3A': embryonic day 7.5; Elof1tm1.1(KOMP)Vlcg/Elof1+ (more )
Note: Transverse section, taken just above the Em/Ex boundary.
Structure Level Pattern Image Note
TS11: extraembryonic component Absent 3A' OCT4
Specimen 3A'': embryonic day 7.5; Elof1tm1.1(KOMP)Vlcg/Elof1+ (more )
Note: Transverse section.
Structure Level Pattern Image Note
TS11: embryo Present Regionally restricted 3A'' OCT4 Expression is seen in the uncommitted epiblast and newly-formed primitive streak cells.
Specimen 3B': embryonic day 7.5; Elof1tm1.1(KOMP)Vlcg/Elof1tm1.1(KOMP)Vlcg (more )
Note: Transverse section, taken just above the Em/Ex boundary.
Structure Level Pattern Image Note
TS11: extraembryonic component Absent 3B' OCT4
Specimen 3B'': embryonic day 7.5; Elof1tm1.1(KOMP)Vlcg/Elof1tm1.1(KOMP)Vlcg (more )
Note: Transverse section.
Structure Level Pattern Image Note
TS11: embryo Absent 3B'' OCT4 In the mutant the epiblast appears to have either transformed or died.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/30/2025
MGI 6.24 		The Jackson Laboratory