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Gene Expression Data
Assay Details
Assay
Reference: J:229352 Metzger DE, et al., Grg3/TLE3 and Grg1/TLE1 induce monohormonal pancreatic beta-cells while repressing alpha-cell functions. Diabetes. 2014 May;63(5):1804-16
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6441476
Gene symbol: Ins2
Gene name: insulin II
Antibody: anti-insulin
Detection system: Secondary antibody coupled to Alexa Fluor
Assay notes: The specificity of the antibody used was not detailed; both family members have been annotated.
Results
Specimen 1B left: postnatal adult (more )
Note: Double labeled: green - Ins1 and Ins2; gray - Tle3 (assay MGI:6441180).
Structure Level Pattern Image Note
TS28: islets of Langerhans Present 1B middle left Virtually every insulin-positive beta-cell expressed Tle3. The percentage of Tle3+ beta-cells increased from E17.5 (84%) to the adult (98%).
Specimen S2A: embryonic day 14.5 (more )
Note: Triple labeled: green - Ins1 and Ins2; gray - Pdx1 (assay MGI:6441184); red - Gcg (assay MGI:6441183).
Structure Level Pattern Image Note
TS22: pancreas Present S2A Expressed in beta-cells. Not coexpressed with Gcg.
Specimen S2B: embryonic day 14.5; Tle3tm1Jcc/Tle3+ (more )
Note: Triple labeled: green - Ins1 and Ins2; gray - Pdx1 (assay MGI:6441184); red - Gcg (assay MGI:6441183).
Structure Level Pattern Image Note
TS22: pancreas Present S2B A few endocrine cells were bihormonal (coexpressed Insulin and Gcg).
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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