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Gene Expression Data
Assay Details
Assay
Reference: J:245773 Paikari A, et al., The eutheria-specific miR-290 cluster modulates placental growth and maternal-fetal transport. Development. 2017 Oct 15;144(20):3731-3743
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6434223
Gene symbol: Mirc5
Gene name: microRNA cluster 5, including Mir290a through Mir295
Reporter: mCherry
Antibody: Anti-mCherry antibody (ab167453)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 1A: embryonic day 7.5; Mirc5tm1.1Blel/Mirc5+ (more )
Structure Level Pattern Image Note
TS11: embryo Present 1A mCherry Expression in the embryo proper began to diminish at E7.5.
TS11: extraembryonic component Present 1A mCherry
Specimen 1B: embryonic day 8.5; Mirc5tm1.1Blel/Mirc5+ (more )
Structure Level Pattern Image Note
TS13: embryo Absent 1B mCherry
TS13: chorion Strong 1B mCherry
TS13: ectoplacental cone Strong 1B mCherry
TS13: yolk sac Strong 1B mCherry
Specimen 1C: embryonic day 10.5; Mirc5tm1.1Blel/Mirc5+ (more )
Structure Level Pattern Image Note
TS17: embryo Absent 1C mCherry
TS17: yolk sac Present 1C mCherry
TS28: placenta Present 1C mCherry
Specimen 1D embryo: embryonic day 15.5; Mirc5tm1.1Blel/Mirc5+ (more )
Structure Level Pattern Image Note
TS23: embryo Absent 1D embryo mCherry
Specimen 1D placenta: embryonic day 15.5; Mirc5tm1.1Blel/Mirc5+ (more )
Structure Level Pattern Image Note
TS28: placenta Present 1D placenta mCherry
Specimen 2A: embryonic day 7.5; Mirc5tm1.1Blel/Mirc5+ (more )
Structure Level Pattern Image Note
TS11: extraembryonic component Strong 2A mCherry
TS11: embryo Present 2A mCherry
TS11: chorion Present 2A mCherry
TS11: ectoplacental cone Present 2A mCherry
Specimen 2B: embryonic day 9.5; Mirc5tm1.1Blel/Mirc5+ (more )
Structure Level Pattern Image Note
TS15: chorion Present 2B mCherry
TS15: ectoplacental cone Present 2B mCherry
TS15: extraembryonic component Present 2B mCherry
TS15: embryo Absent 2B mCherry
Specimen 2C: embryonic day 18.5; Mirc5tm1.1Blel/Mirc5+ (more )
Structure Level Pattern Image Note
TS26: labyrinthine zone Present 2C bottom mCherry
TS26: trophoblast giant cell Present Regionally restricted 2C higher mag center , 2C bottom mCherry , 2C higher mag right Expression was detected in the parietal trophoblast giant cells.
TS26: spongiotrophoblast Absent 2C bottom mCherry
Specimen S1D: embryonic day 8.5; Mirc5tm1.1Blel/Mirc5+ (more )
Structure Level Pattern Image Note
TS13: embryo Absent S1D mCherry
TS13: chorion Strong S1D mCherry
TS13: ectoplacental cone Strong S1D mCherry
TS13: yolk sac Strong S1D mCherry
Specimen S1E: embryonic day 9.5; Mirc5tm1.1Blel/Mirc5+ (more )
Structure Level Pattern Image Note
TS15: embryo Absent S1E mCherry
TS15: chorion Strong S1E mCherry
TS15: ectoplacental cone Strong S1E mCherry
TS15: yolk sac Strong S1E mCherry
Specimen S1F: embryonic day 12.5; Mirc5tm1.1Blel/Mirc5+ (more )
Structure Level Pattern Image Note
TS20: embryo Absent S1F mCherry
TS20: yolk sac Present S1F mCherry
TS28: placenta Present S1F mCherry
Specimen S1G placenta: embryonic day 18.5; Mirc5tm1.1Blel/Mirc5+ (more )
Structure Level Pattern Image Note
TS28: placenta Present S1G mCherry placenta
Specimen S1G embryo: embryonic day 18.5; Mirc5tm1.1Blel/Mirc5+ (more )
Structure Level Pattern Image Note
TS26: embryo Absent S1G mCherry embryo
Specimen S2A: embryonic day 8.5; Mirc5tm1.1Blel/Mirc5+ (more )
Structure Level Pattern Image Note
TS13: chorion Present S2A mCherry
TS13: ectoplacental cone Present S2A mCherry
TS13: yolk sac Present S2A mCherry
TS28: placenta Present S2A mCherry
TS13: embryo Absent S2A mCherry
Specimen S2B E10.5: embryonic day 10.5; Mirc5tm1.1Blel/Mirc5+ (more )
Structure Level Pattern Image Note
TS17: labyrinthine zone Present S2B E10.5
TS17: trophoblast giant cell Present Regionally restricted S2B E10.5 Expression was detected in parietal trophoblast giant cells.
TS17: spongiotrophoblast Absent S2B E10.5
Specimen S2B E12.5: embryonic day 12.5; Mirc5tm1.1Blel/Mirc5+ (more )
Structure Level Pattern Image Note
TS20: labyrinthine zone Present S2B E12.5
TS20: trophoblast giant cell Present Regionally restricted S2B E12.5 Expression was detected in parietal trophoblast giant cells.
TS20: spongiotrophoblast Absent S2B E12.5
Specimen S2B E15.5: embryonic day 15.5; Mirc5tm1.1Blel/Mirc5+ (more )
Structure Level Pattern Image Note
TS23: labyrinthine zone Present S2B E15.5
TS23: trophoblast giant cell Present Regionally restricted S2B higher mag center , S2B higher mag right , S2B higher mag left , S2B E15.5 Expression was detected in parietal trophoblast giant cells.
TS23: spongiotrophoblast Absent S2B higher mag center , S2B higher mag right , S2B E15.5 , S2B higher mag left
Specimen S2C E18.5: embryonic day 18.5; Mirc5tm1.1Blel/Mirc5+ (more )
Note: Double labeled: red - Mirc5; green - Tpbpa (assay MGI:6434318).
Structure Level Pattern Image Note
TS26: labyrinthine zone Present S2C E18.5
TS26: trophoblast giant cell Present Regionally restricted S2C E18.5 Expression was detected in parietal trophoblast giant cells.
TS26: spongiotrophoblast Absent S2C E18.5
Specimen S2C E10.5: embryonic day 10.5; Mirc5tm1.1Blel/Mirc5+ (more )
Note: Double labeled: red - Mirc5; green - Tpbpa (assay MGI:6434318).
Structure Level Pattern Image Note
TS17: labyrinthine zone Present S2C E10.5
TS17: trophoblast giant cell Present Regionally restricted S2C E10.5 Expression was detected in parietal trophoblast giant cells.
TS17: spongiotrophoblast Absent S2C E10.5
Specimen S2D: embryonic day 18.5; Mirc5tm1.1Blel/Mirc5+ (more )
Note: Double labeled: red - Mirc5; green - Pecam1 (assay MGI:6434319).
Structure Level Pattern Image Note
TS26: labyrinthine zone Present Regionally restricted S2D top , S2D higher mag left , S2D higher mag right , S2D higher mag center Reporter expression was detected in trophoblastic cells of the labyrinth, but allantois-derived endothelial cells (Pecam1-positive) were negative for the reporter.
Specimen S2F: embryonic day 7.5; Mirc5tm1.1Blel/Mirc5+ (more )
Note: Double labeled: red - Mirc5; green - Prl3d1 (assay MGI:6434320).
Structure Level Pattern Image Note
TS11: trophoblast giant cell Present Regionally restricted S2F b center , S2F a lower left , S2F a top right , S2F b bottom , S2F E7.5 , S2F a top left Expression was detected in parietal trophoblast giant cells colocalized with Prl3d1.
Specimen S2G: embryonic day 10.5; Mirc5tm1.1Blel/Mirc5+ (more )
Note: Double labeled: red - Mirc5; green - Prl3d1 (assay MGI:6434320).
Structure Level Pattern Image Note
TS17: trophoblast giant cell Present Regionally restricted S2G bottom , S2G higher mag center , S2G higher mag left Expression was detected in parietal trophoblast giant cells colocalized with Prl3d1.
Specimen S3A embryo: embryonic day 10.5; Mirc5tm1.1Blel/Mirc5+ (more )
Structure Level Pattern Image Note
TS17: primordial germ cell Present S3A Embryo
Specimen S3A placenta: embryonic day 10.5; Mirc5tm1.1Blel/Mirc5+ (more )
Structure Level Pattern Image Note
TS28: placenta Present S3A Placenta
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/28/2024
MGI 6.13 		The Jackson Laboratory