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Gene Expression Data
Assay Details
Assay
Reference: J:285730 Major AT, et al., Dynamic paraspeckle component localisation during spermatogenesis. Reproduction. 2019 Sep;158(3):267-280
Assay type: RNA in situ
MGI Accession ID: MGI:6430505
Gene symbol: Neat1
Gene name: nuclear paraspeckle assembly transcript 1 (non-protein coding)
Probe: Neat1 FISH probe
Probe preparation: Antisense labelled with other - see notes RNA
Assay notes: This is a FISH assay; the probe was labeled with Quasar 570. The probe is to the 5' segment that is shared by both the long and short transcripts, although this experiment indicates it is primarily detecting the long transcript.
Results
Specimen 6Ai: postnatal adult (more )
Structure Cell Type Level Pattern Image Note
TS28: Leydig cell Leydig cell Present 6Ai , 6Aii Bright nuclear foci are observed.
TS28: germ cell of testis Absent 6Ai , 6Aii
TS28: Sertoli cell Sertoli cell Absent 6Aii , 6Ai
TS28: peritubular myoid cell peritubular myoid cell Absent 6Aii , 6Ai
Specimen 6Aiii: postnatal adult (more )
Note: Double labeled: red - Neat1; green - Pspc1 (assay MGI:6430506)
Structure Cell Type Level Pattern Image Note
TS28: Leydig cell Leydig cell Present 6Aiv , 6Aiii Bright nuclear foci are observed.
TS28: germ cell of testis Absent 6Aiii , 6Aiv
TS28: Sertoli cell Sertoli cell Absent 6Aiii , 6Aiv
TS28: peritubular myoid cell peritubular myoid cell Absent 6Aiv , 6Aiii
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
MGI 6.23 		The Jackson Laboratory