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Gene Expression Data
Assay Details
Assay
Reference: J:241694 Gingras J, et al., Synaptic transmission is impaired at neuronal autonomic synapses in agrin-null mice. Dev Neurobiol. 2007 Apr;67(5):521-34
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6416327
Gene symbol: Chrnb4
Gene name: cholinergic receptor, nicotinic, beta polypeptide 4
Antibody: anti-beta2/beta4
Detection system: Secondary antibody coupled to rhodamine
Assay notes: The antibody used can detect both subunits, both family members have been annotated.
Results
Specimen 2a wild type: embryonic day 18.5 (more )
Note: Double labeled: red - Chrnb2 and Chrnb4; green - Syp (assay MGI:6416301).
Structure Level Pattern Image Note
TS26: superior cervical ganglion Present 2a upper right , 2a upper middle Expressed as postsynaptic aggregates, colocalized with some synaptophysin-labeled nerve terminals.
Specimen 2a AGD-/-: embryonic day 18.5; Agrntm3Jrs/Agrntm3Jrs (more )
Note: Double labeled: red - Chrnb2 and Chrnb4; green - Syp (assay MGI:6416301).
Structure Level Pattern Image Note
TS26: superior cervical ganglion Present 2a lower middle , 2a lower right Expressed as postsynaptic aggregates. Significantly fewer synapse number (coexpressed Syp and Chrnb2/b4).
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
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