Antibody:

Robo3 antibody (AF3076)

Structure	Level	Pattern	Image	Note
TS14: embryo	Absent

Structure	Level	Pattern	Image	Note
TS15: midbrain-hindbrain junction	Absent		1b	Expression in rhombencephalon, except rhombomeres 0/1, 3 and 4.
TS15: rhombomere 1	Absent		1b	Expression in rhombencephalon, except rhombomeres 0/1, 3 and 4.
TS15: rhombomere 3	Absent		1b	Expression in rhombencephalon, except rhombomeres 0/1, 3 and 4.
TS15: rhombomere 4	Absent		1b	Expression in rhombencephalon, except rhombomeres 0/1, 3 and 4.
TS15: future hindbrain	Present	Regionally restricted	1b	Expression in axons extending ventrally toward ventral midline.

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Present	Regionally restricted	1c	Expression in longitudinal axons, belonging to the medial longitudinal fasciculus, extending caudally from diencephalon to rhombencephalon. Expression in few cells extending axons ipsilaterally toward rostral rhombencephalon.

Structure	Level	Pattern	Image	Note
TS15: future hindbrain	Present	Regionally restricted	1d	Expression in caudal rhombencephalon. Expression in a few dorsally-located neurons extending axons ventrally toward midline. Expression in cell bodies, axons and growth cones.

Structure	Level	Pattern	Image	Note
TS16: future midbrain	Strong	Regionally restricted	1e	Strong expression in commissural axons of midbrain. Expression tectobulbar/tectospinal axons in mesencephalon (and future colliculi) which cross the midline.
TS16: future hindbrain	Strong	Widespread	1e	Expression extending to entire rhombencephalon, except medial rhombomeres. Significant increase in number of expressing axons, now present almost all along the rhombencephalon.
TS16: future spinal cord	Present	Regionally restricted	1e	Expression in rostral spinal cord.

Structure	Level	Pattern	Image	Note
TS16: future brain	Present	Regionally restricted	1f	Expression in a few ipsilateral axons of medial longitudinal fasciculus projecting from midbrain to rhombencephalon.
TS16: midbrain-hindbrain junction	Present		1f	Expression at isthmus (rhombomere 0-rhombomere 1 boundary).
TS16: future hindbrain	Strong	Regionally restricted	1f	Strong expression in commissural axons rhombencephalon.

Structure	Level	Pattern	Image	Note
TS16: future spinal cord	Present	Regionally restricted	1g	Expression in many differentiated neurons of lateral spinal cord. Expression in growth cones of pioneer commissural axons extending toward midline.
TS16: dorsal root ganglion	Absent		1g
TS16: peripheral nervous system	Absent		1g

Structure	Level	Pattern	Image	Note
TS16: future spinal cord	Present	Regionally restricted	1h	Expression in commissural axons extending toward ventral midline.

Structure	Level	Pattern	Image	Note
TS17: forebrain	Weak		1i
TS17: hindbrain	Strong	Regionally restricted	1i	Expression increases in midbrain and hindbrain.
TS17: midbrain	Strong	Regionally restricted	1i	Expression increases in midbrain and hindbrain.
TS17: brain	Present	Regionally restricted	1i	Highly expressed in axons of expanded tectobulbar/spinal tracts.
TS17: future spinal cord	Present	Graded	1i	Expression increases in spinal cord commissural axons, following a high-rostral to low-caudal gradient, extending to caudal levels.

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Weak	Regionally restricted	1j	Low expression in ganglionic eminences.
TS17: hindbrain	Strong	Regionally restricted	1j	Strong expression in commissural midbrain and hindbrain axons.
TS17: midbrain	Strong	Regionally restricted	1j	Strong expression in commissural midbrain and hindbrain axons.
TS17: brain	Present	Regionally restricted	1j	Expression in a few medial longitudinal fasciculus axons. Highly expressed in axons of expanded tectobulbar/spinal tracts.

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Weak	Regionally restricted	1k	Low expression in ganglionic eminences.
TS17: hindbrain	Strong	Regionally restricted	1k	Strong expression in commissural midbrain and hindbrain axons.
TS17: midbrain	Strong	Regionally restricted	1k	Strong expression in commissural midbrain and hindbrain axons.
TS17: midbrain-hindbrain junction	Absent		1k	Expression at all levels of rhombencephalon except in r0 (isthmus)/r1.
TS17: brain	Present	Regionally restricted	1k	Expression in a few medial longitudinal fasciculus axons. Highly expressed in axons of expanded tectobulbar/spinal tracts.

Structure	Level	Pattern	Image	Note
TS17: brain	Present	Regionally restricted	1l	Strong expression in commissural axons extending toward ventral midline.
TS17: peripheral nervous system	Absent		1l

Structure	Level	Pattern	Image	Note
TS19: forebrain	Strong	Regionally restricted	1m	Strong expression in a few regions in the forebrain. Expression in ventral pallium, at levels of medial and lateral ganglionic eminences.
TS19: future spinal cord	Strong	Regionally restricted	1m	Strong expression in commissures all along spinal cord, hindbrain and midbrain.
TS19: hindbrain	Strong	Regionally restricted	1m	Strong expression in commissures all along spinal cord, hindbrain and midbrain.
TS19: midbrain	Strong	Regionally restricted	1m	Strong expression in commissures all along spinal cord, hindbrain and midbrain.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	1n	Expression in telencephalon including lateral and medial ganglionic eminences, cortex, and few olfactory nerve axons. Expression in interneurons migrating toward the cortex.
TS19: diencephalon	Present	Regionally restricted	1n	Expression in fasciculus retroflexus and other ventral tracts of diencephalon.

Structure	Level	Pattern	Image	Note
TS20: ganglionic eminence	Weak		1o
TS20: telencephalon	Present	Regionally restricted	1o	Expression in interneurons migrating toward the cortex.
TS20: hindbrain	Present	Widespread	1o	Expression is continuous from spinal cord to midbrain.
TS20: midbrain	Present	Widespread	1o	Expression is continuous from spinal cord to midbrain.
TS20: spinal cord	Present	Widespread	1o	Expression is continuous from spinal cord to midbrain.
TS20: cerebellum primordium	Present		1o	Expression in cerebellar plate.

Structure	Level	Pattern	Image	Note
TS20: ganglionic eminence	Present		1p
TS20: olfactory nerve	Present	Regionally restricted	1p	Expression in a few olfactory sensory axons forming the olfactory nerve and projecting into olfactory bulb primordium.

Structure	Level	Pattern	Image	Note
TS22: forebrain	Ambiguous		2a	Expression almost absent from forebrain.
TS22: medial habenular nucleus	Present		2a
TS22: cerebellum	Present	Regionally restricted	2a	Expression in commissural axons in mesencephalon, hindbrain, including the cerebellum, and spinal cord.
TS22: hindbrain	Present	Regionally restricted	2a	Expression in commissural axons in mesencephalon, hindbrain, including the cerebellum, and spinal cord.
TS22: midbrain	Present	Regionally restricted	2a	Expression in commissural axons in mesencephalon, hindbrain, including the cerebellum, and spinal cord.
TS22: spinal cord	Present	Regionally restricted	2a	Expression in commissural axons in mesencephalon, hindbrain, including the cerebellum, and spinal cord.

Structure	Level	Pattern	Image	Note
TS22: telencephalon	Ambiguous		2b	Expression almost absent from forebrain.
TS22: fasciculus retroflexus	Strong		2b	Authors state that the fasciculus retroflexus was the only tract rostral to midbrain with high levels of expression.
TS22: deep cerebellar nucleus	Present	Regionally restricted	2b	Expression in axons of deep cerebellar nuclei which project to ventral medulla.
TS22: cerebellum ventricular layer	Present		2b
TS22: brainstem nucleus	Present	Regionally restricted	2b	Expression in more caudally nigrating neurons of lateral reticular nucleus.

Structure	Level	Pattern	Image	Note
TS24: fasciculus retroflexus	Present		2e
TS24: brainstem nucleus	Present	Regionally restricted	2e	Expression in anterior extramural migratory stream (Aems) containing tangentially migrating pontine neurons.
TS24: cerebellum	Weak		2e	Expression strongly down-regulated in spinal cord, hindbrain and cerebellum.
TS24: hindbrain	Weak		2e	Expression strongly down-regulated in spinal cord, hindbrain and cerebellum.
TS24: spinal cord	Weak		2e	Expression strongly down-regulated in spinal cord, hindbrain and cerebellum.

Structure	Level	Pattern	Image	Note
TS24: medial habenular nucleus	Present	Regionally restricted	2i	Expression in cell bodies from medial habenula and their axons forming fasciculus retroflexus.
TS24: fasciculus retroflexus	Present	Regionally restricted	2i	Increased size of expression domain in fasciculus retroflexus. Fasciculus retroflexus neurons zig-zag, axons crossing midline floorplate at level of interpeduncular nucleus.

Structure	Level	Pattern	Image	Note
TS27: medial habenular nucleus	Present	Regionally restricted	2j	Expression in cell bodies from medial habenula and their axons forming fasciculus retroflexus.
TS27: fasciculus retroflexus	Present	Regionally restricted	2j	Fasciculus retroflexus neurons zig-zag at midline at level of interpeduncular nucleus.
TS27: pons	Absent		2j

Structure	Level	Pattern	Image	Note
TS16: future spinal cord	Present	Regionally restricted	4e	Expression in brachial spinal cord, expression in pioneer commissural axons in marginal zone and growth cones. Expression in axons growing ventrally at surface. Most advanced axons start to turn medially and cross floor plate.
TS16: neural tube floor plate	Present		4e	Most advanced axons start to turn medially and cross floor plate.

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Present	Regionally restricted	4f	Expression in brachial spinal cord, expression in pioneer commissural axons and growth cones. Axons are fasciculated and pass between motor column and ventricular zone before crossing midline.
TS19: neural tube floor plate	Present		4f	Expression in commissural axons extending within spinal cord, turning toward floor plate and avoiding differentiating motor neurons.

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present	Regionally restricted	4g	Expression in brachial spinal cord, expression in pioneer commissural axons and growth cones.
TS21: spinal cord marginal layer	Present	Regionally restricted	4g	Expression in commissural axons growing in two distinct pathways: one superficial in marginal zone under the pia, the other deep along ventral edge of ventricular zone.
TS21: spinal cord ventricular layer	Present	Regionally restricted	4g	Expression in commissural axons growing in two distinct pathways: one superficial in marginal zone under the pia, the other deep along ventral edge of ventricular zone.

Structure	Level	Pattern	Image	Note
TS22: spinal cord	Present	Regionally restricted	4h	Expression in brachial spinal cord, expression in pioneer commissural axons and growth cones.
TS22: spinal cord marginal layer	Weak	Regionally restricted	4h	Expression in commissural axons growing in two distinct pathways: one superficial in marginal zone, the other deep along edge of ventricular zone. Expression only in a few commissural neurons by the time the last spinal cord neurons are generated.
TS22: spinal cord ventricular layer	Weak	Regionally restricted	4h	Expression in commissural axons growing in two distinct pathways: one superficial in marginal zone, the other deep along edge of ventricular zone. Expression only in a few commissural neurons by the time the last spinal cord neurons are generated.

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Present	Widespread	4i	Expression all along rostro-caudal axis of spinal cord. Expressing cells were well aligned and interspaced.

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Present	Widespread	4j	Expression all along rostro-caudal axis of spinal cord. Expressing cells were well aligned and interspaced.

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present	Regionally restricted	4k	Expressing commissural axons followed either a superficial or deep pathway to reach floorplate and avoided motor column.

Structure	Level	Pattern	Image	Note
TS24: fasciculus retroflexus	Strong	Regionally restricted	5a	Strong expression in neurons of medial habenula that project via fascicuuls retroflexus to interpeduncular nucleus, where they cross the midline.
TS24: interpeduncular nucleus	Strong	Regionally restricted	5a	Strong expression in neurons of medial habenula that project via fascicuuls retroflexus to interpeduncular nucleus, where they cross the midline.
TS24: medial habenular nucleus	Strong	Regionally restricted	5a	Strong expression in neurons of medial habenula that project via fascicuuls retroflexus to interpeduncular nucleus, where they cross the midline.

Structure	Level	Pattern	Image	Note
TS24: fasciculus retroflexus	Present	Regionally restricted	5b	Expression in highly fasciculated neurons.

Structure	Level	Pattern	Image	Note
TS24: interpeduncular nucleus	Present	Regionally restricted	5c	Multiple crossing points of habeular axons inside the interpeduncular nucleus.

Structure	Level	Pattern	Image	Note
TS22: fasciculus retroflexus	Present	Regionally restricted	S1a	Expression in neurons of madial habenula and their axons forming the fasciculus retroflexus.
TS22: cerebellum	Present		S1a

Structure	Level	Pattern	Image	Note
TS24: interpeduncular nucleus	Present	Regionally restricted	S1b	Expression on axons projecting to interpeduncular nucleus.
TS24: pons	Present	Regionally restricted	S1b	Expression in migrating pontine neurons.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	6a	Expression in ganglionc eminences.
TS19: olfactory system	Present	Regionally restricted	6a	Expression in olfactory sensory axons in olfactory nerve.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	6b	Expression in ganglionc eminences.
TS19: olfactory system	Present	Regionally restricted	6b	Expression in olfactory sensory axons in olfactory nerve.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	6c	Expression in septum.
TS19: olfactory system	Present	Regionally restricted	6c	Expression in olfactory sensory axons as they enter the olfactory bulb primordium.

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Present	Regionally restricted	6d	Expression in septum.
TS21: main olfactory bulb	Present	Regionally restricted	6d	Expression in olfactory sensory axons as they enter the olfactory bulb primordium.
TS21: olfactory nerve	Present	Regionally restricted	6d	Expression in olfactory sensory axons as they enter the olfactory bulb primordium.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Strong	Regionally restricted	6f	Highly expressed in medial and lateral ganglionic eminences.

Structure	Level	Pattern	Image	Note
TS21: lateral ganglionic eminence	Strong		6g
TS21: medial ganglionic eminence	Strong		6g

Structure	Level	Pattern	Image	Note
TS22: cerebellum	Strong		7a
TS22: hindbrain	Strong		7a
TS22: midbrain	Strong		7a
TS22: deep cerebellar nucleus	Present	Regionally restricted	7a	Expression in ventrally crossing deep nuclei axons.
TS22: spinal cord	Ambiguous		7a	Expression almost absent from spinal cord.

Structure	Level	Pattern	Image	Note
TS22: deep cerebellar nucleus	Present	Regionally restricted	7b	Expression in deep cerebellar nuclei neurons projecting axons toward hindbrain ventral midline. Expression in ventrally crossing deep nuclei axons.
TS22: cerebellum	Present	Regionally restricted	7b	Expression in axons originating from medial nucleus projecting dorsally and crossing cerebellum midline.

Structure	Level	Pattern	Image	Note
TS22: deep cerebellar nucleus	Present	Regionally restricted	7c	Expression in ventrally crossing deep nuclei axons.
TS22: cerebellum ventricular layer	Present		7c
TS22: cerebellum	Present	Regionally restricted	7c	No expression in Purkinje cells.

Structure	Level	Pattern	Image	Note
TS22: cerebellum	Present		8a
TS22: hindbrain	Present	Regionally restricted	8a	Expression in caudal hindbrain associated with migrating neurons of inferior olivary nucleus.

Structure	Level	Pattern	Image	Note
TS22: brainstem nucleus	Present	Regionally restricted	8b	Expression in axons and neurons belonging to the lateral reticular nucleus/external cuneatus nucleus (LRN/ECN) migratory stream crossing midline between inferior olivary nucleus and pial surface.

Structure	Level	Pattern	Image	Note
TS23: hindbrain	Present	Regionally restricted	8c	Expression progressively restricted to anterior extramural migratory stream (Aems).
TS23: brainstem nucleus	Present	Regionally restricted	8c	Expression caudally and rostrally in two superficial migratory stream corresponding to latera reticular nucleus/extenal cuneatus nucleus and pontine nucleus.

Structure	Level	Pattern	Image	Note
TS24: hindbrain	Present	Regionally restricted	8d	Expression progressively restricted to anterior extramural migratory stream (Aems). Highly expressed in Aems containing migrating pontine nucleus neurons.
TS24: brainstem nucleus	Present	Regionally restricted	8d	No expression in neurons of inferior olivary nucleus.

Structure	Level	Pattern	Image	Note
TS24: hindbrain	Present	Regionally restricted	9a	Strong expression in anterior extramural migratory stream (Aems), containing pontine nuclei neurons migrating from dorsal rhombic lip toward ventral midline.
TS24: fasciculus retroflexus	Present		9a

Structure	Level	Pattern	Image	Note
TS24: hindbrain	Present	Regionally restricted	9b	Strong expression in anterior extramural migratory stream (Aems), containing pontine nuclei neurons migrating from dorsal rhombic lip toward ventral midline.
TS24: fasciculus retroflexus	Present		9b

Structure	Level	Pattern	Image	Note
TS24: hindbrain	Present	Regionally restricted	S3a	Highly expressed in pontine nucleus and anterior extramural migratory stream (Aems).
TS24: fasciculus retroflexus	Present		S3a

Structure	Level	Pattern	Image	Note
TS24: hindbrain	Present	Regionally restricted	S3b	Expression in Aems neurons migrating toward ventral midline where they accumulate to form the pontine nuclei.

Structure	Level	Pattern	Image	Note
TS24: hindbrain	Present	Regionally restricted	S3c	Expression in Aems neurons migrating toward ventral midline where they accumulate to form the pontine nuclei.