Gene Expression Data
Assay Details
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Assay
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Reference:
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J:188692
Dixit R, et al., Ascl1 participates in Cajal-Retzius cell development in the neocortex. Cereb Cortex. 2011 Nov;21(11):2599-611
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:6404052
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Gene symbol:
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Neurog2
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Gene name:
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neurogenin 2
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Reporter:
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GFP
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Antibody:
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anti-GFP
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
2I/L:
embryonic day 10.5; Neurog2tm4Fgu/Neurog2 +
(more  )
Note: Double labeled: green - Neurog2 reporter; red - Ascl1 (assay MGI:6404048)
Specimen
2I/L:
(close  )
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Genetic Background:
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involves: CD-1
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Mutant Allele(s):
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Neurog2tm4Fgu/Neurog2+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Neurog2 reporter; red - Ascl1 (assay MGI:6404048)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: telencephalon |
Present |
Single cells |
2L
,
2I
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Expression is seen in proneural cells in the pallium (but not the subpallium).
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Specimen
3I:
embryonic day 11.5; Neurog2tm4Fgu/Neurog2 +
(more )
Note: Double labeled: green - Neurog2 reporter; red - Reln (assay MGI:6404056)
Specimen
3I:
(close )
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Genetic Background:
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involves: CD-1
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Mutant Allele(s):
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Neurog2tm4Fgu/Neurog2+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Neurog2 reporter; red - Reln (assay MGI:6404056)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: telencephalon |
Present |
Regionally restricted |
3I
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Expression is seen in proneural cells, including those in the ventral pallium.
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Specimen
3I':
embryonic day 11.5; Neurog2tm4Fgu/Neurog2 +
(more )
Note: Double labeled: green - Neurog2 reporter; red - Reln (assay MGI:6404056)
Specimen
3I':
(close )
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Genetic Background:
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involves: CD-1
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Mutant Allele(s):
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Neurog2tm4Fgu/Neurog2+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Neurog2 reporter; red - Reln (assay MGI:6404056)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: telencephalon |
Present |
Regionally restricted |
3I'
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Expression is seen in proneural cells, including those in the pallial septum.
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Specimen
3I'':
embryonic day 11.5; Neurog2tm4Fgu/Neurog2 +
(more )
Note: Double labeled: green - Neurog2 reporter; red - Reln (assay MGI:6404056)
Specimen
3I'':
(close )
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Genetic Background:
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involves: CD-1
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Mutant Allele(s):
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Neurog2tm4Fgu/Neurog2+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Neurog2 reporter; red - Reln (assay MGI:6404056)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: telencephalon |
Present |
Regionally restricted |
3I''
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Expression is seen in proneural cells, including those in the cortical hem.
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Specimen
3J:
embryonic day 11.5; Neurog2tm4Fgu/Neurog2 +
(more )
Note: Double labeled: green - Neurog2 reporter; red - Trp73 (assay MGI:6404057)
Specimen
3J:
(close )
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Genetic Background:
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involves: CD-1
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Mutant Allele(s):
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Neurog2tm4Fgu/Neurog2+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Neurog2 reporter; red - Trp73 (assay MGI:6404057)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: telencephalon |
Present |
Regionally restricted |
3J
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Expression is seen in proneural cells, including those in the piriform cortex and ventral pallium.
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Specimen
3J':
embryonic day 11.5; Neurog2tm4Fgu/Neurog2 +
(more )
Note: Double labeled: green - Neurog2 reporter; red - Trp73 (assay MGI:6404057)
Specimen
3J':
(close )
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Genetic Background:
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involves: CD-1
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Mutant Allele(s):
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Neurog2tm4Fgu/Neurog2+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Neurog2 reporter; red - Trp73 (assay MGI:6404057)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: telencephalon |
Present |
Regionally restricted |
3J'
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Expression is seen in proneural cells, including those in the pallial septum.
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Specimen
3J'':
embryonic day 11.5; Neurog2tm4Fgu/Neurog2 +
(more )
Note: Double labeled: green - Neurog2 reporter; red - Trp73 (assay MGI:6404057)
Specimen
3J'':
(close )
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Genetic Background:
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involves: CD-1
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Mutant Allele(s):
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Neurog2tm4Fgu/Neurog2+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Neurog2 reporter; red - Trp73 (assay MGI:6404057)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: telencephalon |
Present |
Regionally restricted |
3J''
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Expression is seen in proneural cells, including those in the cortical hem.
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Specimen
3K:
embryonic day 11.5; Neurog2tm4Fgu/Neurog2 +
(more )
Note: Double labeled: green - Neurog2 reporter; red - Reln (assay MGI:6404056)
Specimen
3K:
(close )
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Genetic Background:
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involves: CD-1
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Mutant Allele(s):
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Neurog2tm4Fgu/Neurog2+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Neurog2 reporter; red - Reln (assay MGI:6404056)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: telencephalon |
Present |
Regionally restricted |
3K
,
3M
,
3L'
,
3L
,
3M'
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Expression is seen in proneural cells, including those in the ventral pallium and basal telencephalon.
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Analysis Tools