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Gene Expression Data
Assay Details
Assay
Reference: J:234927 Orquera DP, et al., Essential function of the transcription factor Rax in the early patterning of the mammalian hypothalamus. Dev Biol. 2016 Aug 1;416(1):212-24
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6392060
Gene symbol: Rax
Gene name: retina and anterior neural fold homeobox
Antibody: anti-RAX
Detection system: Secondary antibody coupled to Cy3
Results
Specimen 7R/S: embryonic day 8.5 (more )
Note: Triple labeled: red - Rax; green - Shh (assay MGI:6392059); blue - DAPI.
Structure Level Pattern Image Note
TS13: future prosencephalon Present Widespread 7R , 7S Broad domain of expression in future forebrain.
Specimen S3A/D/E: embryonic day 9.5 (more )
Note: Triple labeled: red - Rax; green - Shh (assay MGI:6392059); blue - DAPI.
Structure Level Pattern Image Note
TS15: diencephalon Present Regionally restricted S3D , S3E , S3A In dorsomedial terminal hypothalamus, co-expression with Shh at hypothalamic midline.
Specimen S3F/I/J: embryonic day 9.5 (more )
Note: Triple labeled: red - Rax; green - Shh (assay MGI:6392059); blue - DAPI.
Structure Level Pattern Image Note
TS15: diencephalon Present Regionally restricted S3F , S3J , S3I In dorsomedial terminal hypothalamus, co-expression with Shh at hypothalamic midline.
Specimen S3K/N/O: embryonic day 9.5 (more )
Note: Triple labeled: red - Rax; green - Shh (assay MGI:6392059); blue - DAPI.
Structure Level Pattern Image Note
TS15: diencephalon Present Regionally restricted S3O , S3N , S3K In ventromedial terminal hypothalus, expression at hypothalamic midline.
Specimen S3P/S/T: embryonic day 9.5 (more )
Note: Triple labeled: red - Rax; green - Shh (assay MGI:6392059); blue - DAPI.
Structure Level Pattern Image Note
TS15: diencephalon Present Regionally restricted S3S , S3T , S3P In ventromedial terminal hypothalus, expression at hypothalamic midline.
Specimen S4A/D/E: embryonic day 10.5 (more )
Note: Triple labeled: red - Rax; green - Shh (assay MGI:6392059); blue - DAPI.
Structure Level Pattern Image Note
TS17: diencephalon Present Regionally restricted S4E , S4A , S4D In dorsomedial terminal hypothalamus, co-expression with Shh at hypothalamic midline.
Specimen S4F/I/J: embryonic day 10.5 (more )
Note: Triple labeled: red - Rax; green - Shh (assay MGI:6392059); blue - DAPI.
Structure Level Pattern Image Note
TS17: diencephalon Present Regionally restricted S4J , S4I , S4F In dorsomedial terminal hypothalamus, co-expression with Shh at hypothalamic midline.
Specimen S4K/N/O: embryonic day 10.5 (more )
Note: Triple labeled: red - Rax; green - Shh (assay MGI:6392059); blue - DAPI.
Structure Level Pattern Image Note
TS17: diencephalon Strong Regionally restricted S4N , S4K , S4O In ventromedial terminal hypothalus, strong expression at hypothalamic midline.
Specimen S4P/S/T: embryonic day 10.5 (more )
Note: Triple labeled: red - Rax; green - Shh (assay MGI:6392059); blue - DAPI.
Structure Level Pattern Image Note
TS17: diencephalon Strong Regionally restricted S4S , S4T , S4P In ventromedial terminal hypothalus, strong expression at hypothalamic midline, including infundibulum.
Specimen S4U/X/Y: embryonic day 10.5 (more )
Note: Triple labeled: red - Rax; green - Shh (assay MGI:6392059); blue - DAPI.
Structure Level Pattern Image Note
TS17: diencephalon Strong Regionally restricted S4U , S4Y , S4X In ventromedial terminal hypothalus, strong expression at hypothalamic midline, including infundibulum.
TS17: infundibular recess of 3rd ventricle Strong Regionally restricted S4U , S4Y , S4X In ventromedial terminal hypothalus, strong expression at hypothalamic midline, including infundibulum.
Specimen S5A/D/E: embryonic day 11.5 (more )
Note: Triple labeled: red - Rax; green - Shh (assay MGI:6392059); blue - DAPI.
Structure Level Pattern Image Note
TS19: diencephalon Present Regionally restricted S5E , S5A , S5D No longer expressed in most of dorsomedial terminal hypothalamus.
Specimen S5F/I/J: embryonic day 11.5 (more )
Note: Triple labeled: red - Rax; green - Shh (assay MGI:6392059); blue - DAPI.
Structure Level Pattern Image Note
TS19: diencephalon Present Regionally restricted S5I , S5J , S5F No longer expressed in most of dorsomedial terminal hypothalamus.
Specimen S5K/N/O: embryonic day 11.5 (more )
Note: Triple labeled: red - Rax; green - Shh (assay MGI:6392059); blue - DAPI.
Structure Level Pattern Image Note
TS19: diencephalon Present Regionally restricted S5K , S5N , S5O In ventromedial terminal hypothalus, strong expression at hypothalamic midline, including infundibulum.
TS19: infundibular recess of 3rd ventricle Present Regionally restricted S5K , S5N , S5O In ventromedial terminal hypothalus, strong expression at hypothalamic midline, including infundibulum.
Specimen S5P/S/T: embryonic day 11.5 (more )
Note: Triple labeled: red - Rax; green - Shh (assay MGI:6392059); blue - DAPI.
Structure Level Pattern Image Note
TS19: diencephalon Present Regionally restricted S5P , S5T , S5S In ventromedial terminal hypothalus, strong expression at hypothalamic midline.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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