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Gene Expression Data
Assay Details
Assay
Reference: J:237528 Lilly AJ, et al., Interplay between SOX7 and RUNX1 regulates hemogenic endothelial fate in the yolk sac. Development. 2016 Dec 01;143(23):4341-4351
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6392035
Gene symbol: Runx1
Gene name: runt related transcription factor 1
Antibody: anti-RUNX1/AML1 + RUNX2 + RUNX3 (EPR3099; ab92336)
Detection system: Secondary antibody coupled to Alexa Fluor 647
Assay notes: The specificity of the antibody used was not detailed; all family members have been annotated. Expression showed similar patterns to proximal Runx1-RFP expression, which demonstrated that Runx1 is the prevalent Runx factor expressed in the yolk sac at this stage.
Results
Specimen 2B: embryonic day 8.5 (more )
Note: Double labeled: purple - pan-Runx; green - Sox7 (assay MGI:6392025).
Structure Level Pattern Image Note
TS13: yolk sac Present Regionally restricted 2B Merge , 2B Runx Expressed mainly around the blood island regions. Co-expressed with Sox7 in endothelial cells on the edge of the blood island.
TS13: blood island Present Regionally restricted 2B Runx , 2B Merge Co-expressed with Sox7 in endothelial cells on the edge of the blood island.
Specimen 2C: embryonic day 8.5 (more )
Note: Triple labeled: purple - pan-Runx; white - Pecam1 (assay MGI:6392036); green - Sox7 (assay MGI:6392025).
Structure Level Pattern Image Note
TS13: yolk sac Present Regionally restricted 2C Merge , 2C Runx Expressed mainly around the blood island regions. Co-expressed with Sox7 in endothelial cells on the edge of the blood island.
TS13: blood island Present Regionally restricted 2C Merge , 2C Runx Co-expressed with Sox7 in endothelial cells on the edge of the blood island.
Specimen 2D: embryonic day 9.5 (more )
Note: Triple labeled: purple - pan-Runx; white - Pecam1 (assay MGI:6392036); green - Sox7 (assay MGI:6392025).
Structure Level Pattern Image Note
TS15: yolk sac blood vessel Present 2D Runx , 2D Merge More Sox7-coexpressing endothelial cells.
TS15: yolk sac Present Clusters 2D Merge , 2D Runx Lower expression in Sox7-coexpressing cells than cells in emerging hematopoietic clusters.
Specimen 2E: embryonic day 9.5 (more )
Note: Triple labeled: purple - pan-Runx; white - Pecam1 (assay MGI:6392036); green - Sox7 (assay MGI:6392025).
Structure Level Pattern Image Note
TS15: dorsal aorta Present Regionally restricted 2E Runx , 2E Merge Coexpressed with Sox7 in endothelial cells.
Specimen S2B: embryonic day 8.5 (more )
Note: Triple labeled: purple - pan-Runx; white - Pecam1 (assay MGI:6392036); green - Sox7 (assay MGI:6392025).
Structure Level Pattern Image Note
TS13: yolk sac Present Regionally restricted S2B Merge , S2B Runx Expressed mainly around the blood island regions. Co-expressed with Sox7 in endothelial cells on the edge of the blood island.
TS13: blood island Present Regionally restricted S2B Runx , S2B Merge Co-expressed with Sox7 in endothelial cells on the edge of the blood island.
Specimen S2C: embryonic day 9.5 (more )
Note: Triple labeled: purple - pan-Runx; white - Pecam1 (assay MGI:6392036); green - Sox7 (assay MGI:6392025).
Structure Level Pattern Image Note
TS15: yolk sac Present Clusters S2C Merge , S2C Runx Expressed in clusters throughout the yolk sac.
TS15: yolk sac blood vessel Present S2C Runx , S2C Merge More Sox7-coexpressing endothelial cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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