About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:228519 Jia S, et al., Insm1 controls the differentiation of pulmonary neuroendocrine cells by repressing Hes1. Dev Biol. 2015 Dec 1;408(1):90-8
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6391189
Gene symbol: Insm1
Gene name: insulinoma-associated 1
Reporter: lacZ
Antibody: anti-Insm1
Assay notes: Two antibodies were described, but the one used in this assay was not detailed. Anti-beta-galactosidase MGI:6391181 may have been used in this assay.
Results
Specimen 1B +/lacZ (upper): embryonic day 18.5; Insm1tm1Cbm/Insm1+ (more )
Note: Double labeled: red - Insm1 reporter; green - Pgp9.5 (Uchl1 assay MGI:6391191).
Structure Level Pattern Image Note
TS26: lung epithelium Present Regionally restricted 1B +/lacZ (upper) Expression in neuroendocrine cells of distal lung.
Specimen 1B lacZ/lacZ (upper): embryonic day 18.5; Insm1tm1Cbm/Insm1tm1Cbm (more )
Note: Double labeled: red - Insm1 reporter; green - Pgp9.5 (Uchl1 assay MGI:6391191).
Structure Level Pattern Image Note
TS26: lung epithelium Present Regionally restricted 1B lacZ/lacZ (upper) Expression in neuroendocrine cells of distal lung.
Specimen 1B +/lacZ (lower): embryonic day 18.5; Insm1tm1Cbm/Insm1+ (more )
Note: Double labeled: red - Insm1 reporter; green - CGRP (Calca assay MGI:6391192).
Structure Level Pattern Image Note
TS26: lung epithelium Present Regionally restricted 1B +/lacZ (lower) Expression in neuroendocrine cells of distal lung.
Specimen 1B lacZ/lacZ (lower): embryonic day 18.5; Insm1tm1Cbm/Insm1tm1Cbm (more )
Note: Double labeled: red - Insm1 reporter; green - CGRP (Calca assay MGI:6391192).
Structure Level Pattern Image Note
TS26: lung epithelium Present Regionally restricted 1B lacZ/lacZ (lower)
Specimen 1C +/lacZ (upper): embryonic day 18.5; Insm1tm1Cbm/Insm1+ (more )
Note: Double labeled: red - Insm1 reporter; green - Pgp9.5 (Uchl1 assay MGI:6391191).
Structure Level Pattern Image Note
TS26: lung epithelium Present Regionally restricted 1C +/lacZ (upper) Expression in proximal and distal lung epithelium.
Specimen 1C lacZ/lacZ (upper): embryonic day 18.5; Insm1tm1Cbm/Insm1tm1Cbm (more )
Note: Double labeled: red - Insm1 reporter; green - Pgp9.5 (Uchl1 assay MGI:6391191).
Structure Level Pattern Image Note
TS26: lung epithelium Present Regionally restricted 1C lacZ/lacZ (upper) Expression in proximal and distal lung epithelium.
Specimen 1C +/lacZ (lower): embryonic day 18.5; Insm1tm1Cbm/Insm1+ (more )
Note: Double labeled: red - Insm1 reporter; green - CGRP (Calca assay MGI:6391192).
Structure Level Pattern Image Note
TS26: lung epithelium Present Regionally restricted 1C +/lacZ (lower) Expression in proximal and distal lung epithelium.
Specimen 1C lacZ/lacZ (lower): embryonic day 18.5; Insm1tm1Cbm/Insm1tm1Cbm (more )
Note: Double labeled: red - Insm1 reporter; green - CGRP (Calca assay MGI:6391192).
Structure Level Pattern Image Note
TS26: lung epithelium Present Regionally restricted 1C lacZ/lacZ (lower) Expression in proximal and distal lung epithelium.
Specimen 2A +/lacZ: embryonic day 14.5; Insm1tm1Cbm/Insm1+ (more )
Note: Double labeled: red - Insm1 reporter; green - Pgp9.5 (Uchl1 assay MGI:6391191).
Structure Level Pattern Image Note
TS22: lung epithelium Present 2A +/lacZ (left)
Specimen 2A lacZ/lacZ: embryonic day 14.5; Insm1tm1Cbm/Insm1tm1Cbm (more )
Note: Double labeled: red - Insm1 reporter; green - Pgp9.5 (Uchl1 assay MGI:6391191).
Structure Level Pattern Image Note
TS22: lung epithelium Present 2A lacZ/lacZ (left)
Specimen 2B +/lacZ: embryonic day 16.5; Insm1tm1Cbm/Insm1+ (more )
Note: Double labeled: red - Insm1 reporter; green - Pgp9.5 (Uchl1 assay MGI:6391191).
Structure Level Pattern Image Note
TS24: lung epithelium Present 2B +//lacZ (left)
Specimen 2B lacZ/lacZ: embryonic day 16.5; Insm1tm1Cbm/Insm1tm1Cbm (more )
Note: Double labeled: red - Insm1 reporter; green - Pgp9.5 (Uchl1 assay MGI:6391191).
Structure Level Pattern Image Note
TS24: lung epithelium Present 2B lacZ/lacZ (left)
Specimen 2C +/lacZ: embryonic day 18.5; Insm1tm1Cbm/Insm1+ (more )
Note: Double labeled: red - Insm1 reporter; green - Pgp9.5 (Uchl1 assay MGI:6391191).
Structure Level Pattern Image Note
TS26: lung epithelium Present 2C +/lacZ (left)
Specimen 2C lacZ/lacZ: embryonic day 18.5; Insm1tm1Cbm/Insm1tm1Cbm (more )
Note: Double labeled: red - Insm1 reporter; green - Pgp9.5 (Uchl1 assay MGI:6391191).
Structure Level Pattern Image Note
TS26: lung epithelium Present 2C lacZ/lacZ (left)
Specimen 3A +/lacZ: embryonic day 13.5; Insm1tm1Cbm/Insm1+ (more )
Note: Double labeled: red - Insm1 reporter; green - Hes1 (assay MGI:6391193).
Structure Level Pattern Image Note
TS21: lung epithelium Present 3A +/lacZ
Specimen 3A lacZ/lacZ: embryonic day 13.5; Insm1tm1Cbm/Insm1tm1Cbm (more )
Note: Double labeled: red - Insm1 reporter; green - Hes1 (assay MGI:6391193).
Structure Level Pattern Image Note
TS21: lung epithelium Present 3A lacZ/lacZ
Specimen 3B +/lacZ: embryonic day 15.5; Insm1tm1Cbm/Insm1+ (more )
Note: Double labeled: red - Insm1 reporter; green - Hes1 (assay MGI:6391193).
Structure Level Pattern Image Note
TS23: lung epithelium Present 3B +/lacZ
Specimen 3B lacZ/lacZ: embryonic day 15.5; Insm1tm1Cbm/Insm1tm1Cbm (more )
Note: Double labeled: red - Insm1 reporter; green - Hes1 (assay MGI:6391193).
Structure Level Pattern Image Note
TS23: lung epithelium Present Regionally restricted 3B lacZ/lacZ Authors report co-expression with Hes1.
Specimen 3C +/lacZ: embryonic day 18.5; Insm1tm1Cbm/Insm1+ (more )
Note: Double labeled: red - Insm1 reporter; green - Hes1 (assay MGI:6391193).
Structure Level Pattern Image Note
TS26: lung epithelium Present 3C +/lacZ
Specimen 3C lacZ/lacZ: embryonic day 18.5; Insm1tm1Cbm/Insm1tm1Cbm (more )
Note: Double labeled: red - Insm1 reporter; green - Hes1 (assay MGI:6391193).
Structure Level Pattern Image Note
TS26: lung epithelium Present Regionally restricted 3C lacZ/lacZ Authors report co-expression with Hes1.
Specimen 4A +/lacZ: embryonic day 18.5; Insm1tm1Cbm/Insm1+ (more )
Note: Triple labeled: red - Insm1 reporter; green - CC10 (Scgb1a1 assay MGI:6391194); blue - DAPI
Structure Level Pattern Image Note
TS26: lung epithelium Present Regionally restricted 4A +//lacZ No co-expression with CC10 (Scgb1a1).
Specimen 4A lacZ/lacZ: embryonic day 18.5; Insm1tm1Cbm/Insm1tm1Cbm (more )
Note: Triple labeled: red - Insm1 reporter; green - CC10 (Scgb1a1 assay MGI:6391194); blue - DAPI
Structure Level Pattern Image Note
TS26: lung epithelium Present Regionally restricted 4A lacZ/lacZ No co-expression with CC10 (Scgb1a1).
Specimen 4B +/lacZ: embryonic day 18.5; Insm1tm1Cbm/Insm1+ (more )
Note: Triple labeled: red - Insm1 reporter; green - SSEA1 (Fut4 assay MGI:6391195); blue - DAPI
Structure Level Pattern Image Note
TS26: lung epithelium Present Regionally restricted 4B +/lacZ No co-expression with SSEA1 (Fut4).
Specimen 4B lacZ/lacZ: embryonic day 18.5; Insm1tm1Cbm/Insm1tm1Cbm (more )
Note: Triple labeled: red - Insm1 reporter; green - SSEA1 (Fut4 assay MGI:6391195); blue - DAPI
Structure Level Pattern Image Note
TS26: lung epithelium Present Regionally restricted 4B lacZ/lacZ No co-expression with SSEA1 (Fut4).
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
05/21/2024
MGI 6.23 		The Jackson Laboratory