Casp3 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:272586 Suzuki T, et al., Postnatal liver functional maturation requires Cnot complex-mediated decay of mRNAs encoding cell cycle and immature liver genes. Development. 2019 Feb 15;146(4):dev168146
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6383458
Gene symbol:	Casp3
Gene name:	caspase 3

Antibody:	anti-cleaved caspase-3 (Asp175) (5A1E; #9664)
Detection system:	Secondary antibody coupled to Alexa Fluor 488
Assay notes:	The antibody recognized the cleaved form of the protein.

Results

S6_Control Cleaved caspase 3 ©

S6_Control Merge left ©

Specimen S6 Control: postnatal week 4 (more

)
Note: The following genotypes could have been used as control samples: (flox/+), (flox/flox), Alb-cre;(+/+) or Alb-cre;(flox/+). Double labeled: green - Casp3; red - Alb (assay MGI:6383453).

Structure	Level	Pattern	Image	Note
TS28: liver	Ambiguous		S6_Control Cleaved caspase 3 , S6_Control Merge left

Specimen S6 Cnot3LKO: postnatal week 4; Cnot3^tm1.2Tya/Cnot3^tm1.2Tya, Speer6-ps1^{Tg(Alb-cre)21Mgn}/0 (more

)
Note: Conditional mutant. Double labeled: green - Casp3; red - Alb (assay MGI:6383453).

Structure	Level	Pattern	Image	Note
TS28: liver	Present		S6_Cnot3LKO Merge left , S6_Cnot3LKO Cleaved caspase 3	The cleaved Caspase 3 immunopositive cells also expressed Albumin.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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