Otx2 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:134435 Anselme I, et al., Defects in brain patterning and head morphogenesis in the mouse mutant Fused toes. Dev Biol. 2007 Apr 1;304(1):208-20
Assay type:	RNA in situ
MGI Accession ID:	MGI:6382587
Gene symbol:	Otx2
Gene name:	orthodenticle homeobox 2

Probe:	Otx2 cDNA12
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

2J ©

Specimen 2J: embryonic day 8.0 (more

)
Note: 7 somites. Double labeled: red - Otx2; purple - Pax6 (assay MGI:6382586).

Structure	Level	Pattern	Image	Note
TS12: future midbrain	Present		2J

2L ©

Specimen 2L: embryonic day 8.5 (more

)
Note: 10 somites. Also stained for Hoxb1 (assay MGI:6382588); both purple.

Structure	Level	Pattern	Image	Note
TS13: future midbrain	Present		2L
TS13: future prosencephalon	Present		2L

2R ©

Specimen 2R: embryonic day 9.5 (more

)
Note: Double labeled: red - Otx2; purple - Pax6 (assay MGI:6382586).

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Present		2R

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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