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Gene Expression Data
Assay Details
Assay
Reference: J:275243 Bosch PJ, et al., A critical role for the nuclear protein Akirin2 in the formation of mammalian muscle in vivo. Genesis. 2019 May;57(5):e23286
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6379290
Gene symbol: Casp3
Gene name: caspase 3
Antibody: anti-cleaved Caspase-3 (Asp175; #9661)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Assay notes: The antibody recognized the cleaved form of the protein.
Results
Specimen 5d Sim1-Cre;Aki2fl/+: embryonic day 10.5; Akirin2tm1Aki/Akirin2+, Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Tg(Sim1-cre)1Lowl/0 (more )
Note: Conditional mutant. Double labeled: green - Casp3; purple - Pax3 (assay MGI:6379291).
Structure Level Pattern Image Note
TS17: dermomyotome Absent 5d CC3 Sim1-Cre;Aki2fl/+ , 5d CC3 Pax3 Sim1-Cre;Aki2fl/+ , 5e CC3 Pax3 Sim1-Cre;Aki2fl/+
Specimen 5d Sim1-Cre;Aki2fl/fl: embryonic day 10.5; Akirin2tm1Aki/Akirin2tm1Aki, Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Tg(Sim1-cre)1Lowl/0 (more )
Note: Conditional mutant. Double labeled: green - Casp3; purple - Pax3 (assay MGI:6379291).
Structure Level Pattern Image Note
TS17: dermomyotome Present Regionally restricted 5d CC3 Sim1-Cre;Aki2fl/fl , 5e CC3 Pax3 Sim1-Cre;Aki2fl/fl , 5d CC3 Pax3 Sim1-Cre;Aki2fl/fl Casp3-immunopositive cells were intermingled with Pax3-immunofluorescence.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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