Gene Expression Data
Assay Details
Assay
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Reference:
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J:147561
Blatt EN, et al., Forkhead transcription factor HFH-4 expression is temporally related to ciliogenesis. Am J Respir Cell Mol Biol. 1999 Aug;21(2):168-76
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6376481
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Gene symbol:
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Foxj1
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Gene name:
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forkhead box J1
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Antibody:
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anti-HNF-4
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Detection system:
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Secondary antibody coupled to Cy3
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Results
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Specimen
2E:
postnatal adult
(more )
Note: Double labeled: red - Foxj1; green - Tubb4a (assay MGI:6376479) and Tubb4b (assay MGI:6376480).
Specimen
2E:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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Note:
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Double labeled: red - Foxj1; green - Tubb4a (assay MGI:6376479) and Tubb4b (assay MGI:6376480).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: lung epithelium |
Present |
Regionally restricted |
2E
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Expression was restricted to ciliated cells as indicated by co-staining with the cilia-specific marker Tubb4.
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Specimen
3C:
postnatal adult
(more )
Note: Double labeled: red - Foxj1; green - Tubb4a (assay MGI:6376479) and Tubb4b (assay MGI:6376480).
Specimen
3C:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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Note:
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Double labeled: red - Foxj1; green - Tubb4a (assay MGI:6376479) and Tubb4b (assay MGI:6376480).
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Specimen
4C:
postnatal adult
(more )
Note: Double labeled: red - Foxj1; green - Tubb4a (assay MGI:6376479) and Tubb4b (assay MGI:6376480).
Specimen
4C:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Female
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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Note:
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Double labeled: red - Foxj1; green - Tubb4a (assay MGI:6376479) and Tubb4b (assay MGI:6376480).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: epithelial layer of oviduct |
Present |
Regionally restricted |
4C
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Expression was detected in ciliated cells colocalized with Tubb4.
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Specimen
4F:
postnatal adult
(more )
Note: Double labeled: red - Foxj1; green - Tubb4a (assay MGI:6376479) and Tubb4b (assay MGI:6376480).
Specimen
4F:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
10% Formalin
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Embedding:
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Paraffin
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Note:
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Double labeled: red - Foxj1; green - Tubb4a (assay MGI:6376479) and Tubb4b (assay MGI:6376480).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: brain ependyma |
Present |
Regionally restricted |
4F
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Expression was detected in ciliated cells colocalized with Tubb4.
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Specimen
5D:
embryonic day 15.5
(more )
Note: Double labeled: red - Foxj1; green - Tubb4a (assay MGI:6376479) and Tubb4b (assay MGI:6376480).
Specimen
5D:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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Note:
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Double labeled: red - Foxj1; green - Tubb4a (assay MGI:6376479) and Tubb4b (assay MGI:6376480).
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: lung epithelium |
Present |
Regionally restricted |
5D
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Expression was detected in approximately half of the proximal airway epithelial cells at E15.5. The ciliated cell marker Tubb4 was first detected at E15.5 and was restricted to a small number of airway epithelial cells that express Foxj1.
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Specimen
not shown:
embryonic day 17.5
(more )
Note: Also stained for Tubb4a (assay MGI:6376479) and Tubb4b (assay MGI:6376480).
Specimen
not shown:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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Note:
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Also stained for Tubb4a (assay MGI:6376479) and Tubb4b (assay MGI:6376480).
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: lung epithelium |
Present |
Regionally restricted |
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Tracking expression in the airway epithelial cells revealed that the number of cells co-expressing Tubb4 and Foxj1 gradually increased at E17.5.
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Specimen
not shown:
embryonic day 18.5
(more )
Note: Also stained for Tubb4a (assay MGI:6376479) and Tubb4b (assay MGI:6376480).
Specimen
not shown:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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Note:
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Also stained for Tubb4a (assay MGI:6376479) and Tubb4b (assay MGI:6376480).
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: lung epithelium |
Present |
Regionally restricted |
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Tracking expression in the airway epithelial cells revealed that the number of cells co-expressing Tubb4 and Foxj1 gradually increased at E18.5.
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Specimen
5F:
postnatal newborn
(more )
Note: Double labeled: red - Foxj1; green - Tubb4a (assay MGI:6376479) and Tubb4b (assay MGI:6376480).
Specimen
5F:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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Note:
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Double labeled: red - Foxj1; green - Tubb4a (assay MGI:6376479) and Tubb4b (assay MGI:6376480).
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: lung epithelium |
Present |
Regionally restricted |
5F
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At birth, expression was detected throughout the proximal airway in a pattern similar to the adult lung. Almost every cell co-expressed Tubb4.
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Specimen
6F:
postnatal adult
(more )
Note: Double labeled: red - Foxj1; green - Tubb4a (assay MGI:6376479) and Tubb4b (assay MGI:6376480).
Specimen
6F:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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Note:
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Double labeled: red - Foxj1; green - Tubb4a (assay MGI:6376479) and Tubb4b (assay MGI:6376480).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: spermatid |
Present |
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6F
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Expression was detected in the nucleus of early spermatids (stage III) and was associated with cytoplasmic expression of Tubb4.
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Specimen
6G:
postnatal adult
(more )
Note: Double labeled: red - Foxj1; green - Tubb4a (assay MGI:6376479) and Tubb4b (assay MGI:6376480).
Specimen
6G:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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Note:
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Double labeled: red - Foxj1; green - Tubb4a (assay MGI:6376479) and Tubb4b (assay MGI:6376480).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: spermatid |
Absent |
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6G
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Expression was absent in spermatozoa and spermatids at stages VII to VIII.
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TS28: spermatozoon |
Absent |
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6G
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Expression was absent in spermatozoa and spermatids at stages VII to VIII.
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Specimen
6H:
postnatal adult
(more )
Note: Double labeled: red - Foxj1; green - Tubb4a (assay MGI:6376479) and Tubb4b (assay MGI:6376480).
Specimen
6H:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
|
Note:
|
Double labeled: red - Foxj1; green - Tubb4a (assay MGI:6376479) and Tubb4b (assay MGI:6376480).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: spermatid |
Absent |
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6H
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Expression was absent in maturing spermatids.
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TS28: spermatocyte |
Present |
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6H
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Expression was detected in the cytoplams of spermatocytes at stages XI through XIII.
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