Gene Expression Data
Assay Details
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Assay
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Reference:
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J:39350
Schneider-Maunoury S, et al., Segmental and neuronal architecture of the hindbrain of Krox-20 mouse mutants. Development. 1997 Mar;124(6):1215-26
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6376253
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Gene symbol:
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Lhx1
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Gene name:
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LIM homeobox protein 1
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Probe:
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clone 31
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Probe preparation:
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Antisense RNA
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Results
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Specimen
2A:
embryonic day 10.5
(more  )
Note: The genetic background of the specimen was either wild type or heterozygous.
Specimen
2A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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The genetic background of the specimen was either wild type or heterozygous.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindbrain |
Present |
Regionally restricted |
2A
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Expression was noted in two longitudinal domains, ventromedial and dorsolateral, as well as a posterior dorsal domain. The ventromedial domain contained six transverse bands that corresponded to inter-rhombomere boundaries. The position of the Lhx1-positive bands were likely at the rhombomere boundaries: r1/r2, r2/r3, r3/r4, r4/r5, r5/r6, and r6/r7.
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Specimen
2B:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6 * DBA/2
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Mutant Allele(s):
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Egr2tm1Pch/Egr2tm1Pch
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindbrain |
Present |
Regionally restricted |
2B
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In the homozygous mutant, the ventromedial domain contained only four transverse bands.
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Specimen
2C:
embryonic day 10.5
(more )
Note: Double labeled: purple - Lhx1; purple - Hoxb1 (assay MGI:6376273). The genetic background of the specimen was either wild type or heterozygous.
Specimen
2C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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Double labeled: purple - Lhx1; purple - Hoxb1 (assay MGI:6376273). The genetic background of the specimen was either wild type or heterozygous.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindbrain |
Present |
Regionally restricted |
2C
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The ventromedial expression domain contained six transverse bands that corresponded to inter-rhombomere boundaries. Compared with the Hoxb1-positive staining in rhombomere 4, the position of the adjacent Lhx1-positive bands were at the rhombomere boundaries: r3/r4 and r4/r5.
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Specimen
2D:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6 * DBA/2
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Mutant Allele(s):
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Egr2tm1Pch/Egr2tm1Pch
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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Double labeled: purple - Lhx1; purple - Hoxb1 (assay MGI:6376273).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindbrain |
Present |
Regionally restricted |
2D
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The ventromedial expression domain in the homozygous mutant contained only four transverse bands that corresponded to inter-rhombomere boundaries. Compared with the Hoxb1-positive staining in rhombomere 4, the position of the four Lhx1-positive bands appeared to correspond to the rhombomere boundaries: r1/r2, r2/r4, r4/r6, and r6/r7.
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Specimen
2E:
embryonic day 10.5; Egr2tm1Pch/Egr2 +
(more )
Note: Double labeled: purple - Lhx1; blue - Egr2 (assay MGI:6376239).
Specimen
2E:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6 * DBA/2
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Mutant Allele(s):
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Egr2tm1Pch/Egr2+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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Double labeled: purple - Lhx1; blue - Egr2 (assay MGI:6376239).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindbrain |
Present |
Regionally restricted |
2E
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The ventromedial expression domain contained six transverse bands that corresponded to inter-rhombomere boundaries. Compared with the Egr2-lacZ staining in rhombomere 5, the flanking Lhx1-positive bands were at the rhombomere boundaries: r4/r5 and r5/r6.
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Analysis Tools