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Gene Expression Data
Assay Details
Assay
Reference: J:210365 Varadkar P, et al., The protein phosphatase 2A B56gamma regulatory subunit is required for heart development. Dev Dyn. 2014 Jun;243(6):778-90
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6361897
Gene symbol: Acta2
Gene name: actin alpha 2, smooth muscle, aorta
Antibody: anti-alpha SMA
Detection system: Secondary antibody coupled to fluorescein
Results
Specimen 4D: embryonic day 10.0 (more )
Note: Double labeled: red - Acta2; green - Ppp2r5c (assay MGI:6361885).
Structure Level Pattern Image Note
TS16: primitive ventricle Present 4D
Specimen 4E: embryonic day 13.0 (more )
Note: Double labeled: red - Acta2; green - Ppp2r5c (assay MGI:6361885).
Structure Level Pattern Image Note
TS21: blood vessel smooth muscle Present 4E
TS21: heart ventricle Present 4E
Specimen 4F: embryonic day 16.0 (more )
Note: Double labeled: red - Acta2; green - Ppp2r5c (assay MGI:6361885).
Structure Level Pattern Image Note
TS24: blood vessel smooth muscle Present 4F
TS24: heart ventricle Present 4F
Specimen 6I: embryonic day 16.0 (more )
Structure Level Pattern Image Note
TS24: left ventricle cardiac muscle Present 6I
Specimen 6J: embryonic day 16.0; Ppp2r5cGt(XP0444)Wtsi/Ppp2r5cGt(XP0444)Wtsi (more )
Structure Level Pattern Image Note
TS24: left ventricle cardiac muscle Present 6J Expression in this mutant is the same as in wild-type.
Specimen 7J: embryonic day 13.0; Ppp2r5cGt(XP0444)Wtsi/Ppp2r5cGt(XP0444)Wtsi (more )
Note: Double labeled with TUNEL (green).
Structure Level Pattern Image Note
TS21: ventricle cardiac muscle Present 7J TUNEL-positive cells do not co-localize with Acta2 cells.
Specimen 7L: embryonic day 16.0; Ppp2r5cGt(XP0444)Wtsi/Ppp2r5cGt(XP0444)Wtsi (more )
Note: Double labeled with TUNEL (green).
Structure Level Pattern Image Note
TS24: ventricle cardiac muscle Present 7L TUNEL-positive cells do not co-localize with Acta2 cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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