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Gene Expression Data
Assay Details
Assay
Reference: J:210365 Varadkar P, et al., The protein phosphatase 2A B56gamma regulatory subunit is required for heart development. Dev Dyn. 2014 Jun;243(6):778-90
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6361885
Gene symbol: Ppp2r5c
Gene name: protein phosphatase 2, regulatory subunit B', gamma
Antibody: B56gamma2 antibody
Detection system: Secondary antibody coupled to fluorescein
Results
Specimen 4A: embryonic day 10.0 (more )
Note: Double labeled with alpha-actinin (red).
Structure Level Pattern Image Note
TS16: primitive ventricle cardiac muscle Strong 4A Areas of expression include the septum.
Specimen 4B: embryonic day 13.0 (more )
Note: Double labeled with alpha-actinin (red).
Structure Level Pattern Image Note
TS21: interventricular septum muscular part Strong 4B
TS21: ventricle cardiac muscle Strong 4B
Specimen 4C: embryonic day 16.0 (more )
Note: Double labeled with alpha-actinin (red).
Structure Level Pattern Image Note
TS24: interventricular septum muscular part Strong 4C
TS24: ventricle cardiac muscle Strong 4C
Specimen 4D: embryonic day 10.0 (more )
Note: Double labeled: green - Ppp2r5c; red - Acta2 (assay MGI:6361897).
Structure Level Pattern Image Note
TS16: primitive ventricle Present 4D
Specimen 4E: embryonic day 13.0 (more )
Note: Double labeled: green - Ppp2r5c; red - Acta2 (assay MGI:6361897).
Structure Level Pattern Image Note
TS21: heart ventricle Present 4E Expression was decreased in the a-smooth muscle actin positive cells in the periphery of the developing ventricles.
Specimen 4F: embryonic day 16.0 (more )
Note: Double labeled: green - Ppp2r5c; red - Acta2 (assay MGI:6361897).
Structure Level Pattern Image Note
TS24: heart ventricle Present 4F Expression was decreased in the a-smooth muscle actin positive cells in the periphery of the developing ventricles.
Specimen 4G: embryonic day 10.0 (more )
Note: Double labeled: green - Ppp2r5c; red - Pecam1 (assay MGI:6361898).
Structure Level Pattern Image Note
TS16: primitive ventricle cardiac muscle Present 4G
TS16: heart endothelium Absent 4G
TS16: primitive ventricle endocardial lining Absent 4G
Specimen 4H: embryonic day 13.0 (more )
Note: Double labeled: green - Ppp2r5c; red - Pecam1 (assay MGI:6361898).
Structure Level Pattern Image Note
TS21: ventricle cardiac muscle Present 4H
TS21: endocardial lining Absent 4H
TS21: heart endothelium Absent 4H
Specimen 4I: embryonic day 16.0 (more )
Note: Double labeled: green - Ppp2r5c; red - Pecam1 (assay MGI:6361898).
Structure Level Pattern Image Note
TS24: ventricle cardiac muscle Present 4I
TS24: endocardial lining Absent 4I
TS24: heart endothelium Absent 4I
Specimen 5A: embryonic day 10.0 (more )
Structure Level Pattern Image Note
TS16: cardiac muscle tissue Present 5A , 5C , 5B Expression is perinuclear and cytoplasmic.
Specimen 5D: embryonic day 16.0 (more )
Structure Level Pattern Image Note
TS24: cardiac muscle tissue Present 5E , 5D , 5F Expression is mostly nuclear.
Specimen 8E: embryonic day 16.0 (more )
Note: Double labeled with BrdU (green).
Structure Level Pattern Image Note
TS24: cardiac muscle tissue Present 8E , 8F
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory