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Gene Expression Data
Assay Details
Assay
Reference: J:273053 Shih HP, et al., A Gene Regulatory Network Cooperatively Controlled by Pdx1 and Sox9 Governs Lineage Allocation of Foregut Progenitor Cells. Cell Rep. 2015 Oct 13;13(2):326-36
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6361504
Gene symbol: Sox2
Gene name: SRY (sex determining region Y)-box 2
Antibody: Anti-Sox2 (Y-17; sc-17320)
Detection system: Secondary antibody coupled to Cy5
Assay notes: Two antibodies were described, but the one used in this assay was not specified; either MGI:5430034 or MGI:5762877 could have been used.
Results
Specimen S3A: embryonic day 9.5 (more )
Note: Anterior - control. Double labeled: red - Sox2; green - Pdx1 (assay MGI:6361503).
Structure Level Pattern Image Note
TS16: stomach Present S3A , S3A'
Specimen S3B: embryonic day 9.5 (more )
Note: Posterior - control. Double labeled: red - Sox2; green - Pdx1 (assay MGI:6361503).
Structure Level Pattern Image Note
TS16: pancreas primordium dorsal bud Absent S3B , S3B'
Specimen S3C: embryonic day 9.5; Pdx1tm2Cvw/Pdx1tm2Cvw, Sox9tm1Gsr/Sox9tm1Gsr, Tg(Foxa3-cre)1Khk/0 (more )
Note: Conditional mutant. Anterior - double mutant. Double labeled: red - Sox2; green - Pdx1 (assay MGI:6361503).
Structure Level Pattern Image Note
TS16: stomach Present S3C' , S3C
Specimen S3D: embryonic day 9.5; Pdx1tm2Cvw/Pdx1tm2Cvw, Sox9tm1Gsr/Sox9tm1Gsr, Tg(Foxa3-cre)1Khk/0 (more )
Note: Conditional mutant. Posterior - double mutant. Double labeled: red - Sox2; green - Pdx1 (assay MGI:6361503).
Structure Level Pattern Image Note
TS16: pancreas primordium dorsal bud Absent S3D' , S3D The combined Pdx1 and Sox9 deletion did not result in ectopic expression of the stomach marker Sox2 in the Pdx1+ domain.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
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