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Gene Expression Data
Assay Details
Assay
Reference: J:258043 Wang Y, et al., N(6)-methyladenosine RNA modification regulates embryonic neural stem cell self-renewal through histone modifications. Nat Neurosci. 2018 Feb;21(2):195-206
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6358383
Gene symbol: Pax6
Gene name: paired box 6
Antibody: anti-Pax6 (#PRB-278P; #30130)
Detection system: Biotinylated secondary antibody/[Strept]avidin coupled to Cy3
Assay notes: The expression signal was amplified with a horseradish-peroxidase-based and Cyanine 3 Tyramide System.
Results
Specimen 1e Mettl14f/f: embryonic day 17.5; Mettl14tm1.1Czhao/Mettl14tm1.1Czhao (more )
Note: Double labeled: red - Pax6; green - Mettl14 (assay MGI:6358350).
Structure Level Pattern Image Note
TS25: cerebral cortex Present 1e Pax6 Mettl14f/f , 1e merge Mettl14f/f
Specimen 1e Mettl14f/f;Nestin-cre: embryonic day 17.5; Mettl14tm1.1Czhao/Mettl14tm1.1Czhao, Tg(Nes-cre)1Kln/0 (more )
Note: Conditional mutant. Double labeled: red - Pax6; green - Mettl14 (assay MGI:6358350).
Structure Level Pattern Image Note
TS25: cerebral cortex Present 1e Pax6 Mettl14f/f;Nestin-cre , 1e merge Mettl14f/f;Nestin-cre
Specimen 1e Mettl14f/+;Nestin-cre: embryonic day 17.5; Mettl14tm1.1Czhao/Mettl14+, Tg(Nes-cre)1Kln/0 (more )
Note: Conditional mutant. Double labeled: red - Pax6; green - Mettl14 (assay MGI:6358350).
Structure Level Pattern Image Note
TS25: cerebral cortex Present 1e merge Mettl14f/+;Nestin-cre , 1e Pax6 Mettl14f/+ Mettl14f/f;Nestin-cre
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
MGI 6.23 		The Jackson Laboratory