Fgf8 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:165806 Toyoda R, et al., FGF8 acts as a classic diffusible morphogen to pattern the neocortex. Development. 2010 Oct;137(20):3439-48
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6358224
Gene symbol:	Fgf8
Gene name:	fibroblast growth factor 8

Antibody:	anti-FGF8
Detection system:	Secondary antibody coupled to horseradish peroxidase
Assay notes:	A TSA Plus Fluorescence System was used to visualize expression.

Results

1A ©

Specimen 1A: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: telencephalon	Present	Regionally restricted	1A	Expression is in the anteromedial telencephalon.
TS15: midbrain-hindbrain junction	Present		1A
TS15: tail	Present		1A

1B ©

Specimen 1B: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: telencephalon	Present	Graded	1B	Expression decreases along anterior/posterior and medial/lateral axes.

1D ©

Specimen 1D: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: telencephalon	Present	Graded	1D	Expression decreases along anterior/posterior and medial/lateral axes.

1E ©

Specimen 1E: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: telencephalon	Present	Graded	1E	Expression decreases along anterior/posterior and medial/lateral axes.

1F ©

Specimen 1F: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: telencephalon	Present	Graded	1F	Expression decreases along anterior/posterior and medial/lateral axes.

1G ©

Specimen 1G: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: telencephalon	Present	Graded	1G	Expression decreases along anterior/posterior and medial/lateral axes.

2A ©

2B ©

Specimen 2A: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: telencephalon	Present	Graded	2A , 2B	Expression is in the neocortical primordium and decreases along anterior/posterior axis.

2E ©

Specimen 2E: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: telencephalon	Present	Regionally restricted	2E	Expression is in the neocortical primordium.

S2B ©

S2D ©

S2E ©

Specimen S2B: embryonic day 11.5 (more

)
Note: Double labeled: green - Fgf8; red - Fgf17 (assay MGI:6358232).

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Punctate	S2B , S2D , S2E	Expression is in the neocortical primordium.

S3A ©

Specimen S3A: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Present	Graded	S3A	Expression is in the neocortical primordium and decreases along anterior/posterior axis.

S3B ©

Specimen S3B: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Present	Graded	S3B	Expression is in the neocortical primordium and decreases along anterior/posterior axis.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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