Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:264526
Dedic N, et al., Chronic CRH depletion from GABAergic, long-range projection neurons in the extended amygdala reduces dopamine release and increases anxiety. Nat Neurosci. 2018 Jun;21(6):803-807
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:6295360
|
Gene symbol:
|
Crh
|
Gene name:
|
corticotropin releasing hormone
|
Probe:
|
Crh cDNA1
|
Probe preparation:
|
Antisense labelled with radioactivity RNA
|
Visualized with:
|
Autoradiography
|
|
Results
|
Specimen
1c ISH Crh - upper:
postnatal week 10-14
(more )
Specimen
1c ISH Crh - upper:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
1c ISH Crh - lower:
postnatal week 10-14
(more )
Specimen
1c ISH Crh - lower:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
1c Gad65/67 + Crh - upper:
postnatal week 10-14
(more )
Note: Double labeled: silver grains - Crh; red - Gad2 (Gad65; assay MGI:6296657) and Gad1 (Gad67; assay MGI:6295980).
Specimen
1c Gad65/67 + Crh - upper:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: silver grains - Crh; red - Gad2 (Gad65; assay MGI:6296657) and Gad1 (Gad67; assay MGI:6295980).
|
Specimen
1c Gad65/67 + Crh - lower:
postnatal week 10-14
(more )
Note: Double labeled: silver grains - Crh; red - Gad2 (Gad65; assay MGI:6296657) and Gad1 (Gad67; assay MGI:6295980).
Specimen
1c Gad65/67 + Crh - lower:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: silver grains - Crh; red - Gad2 (Gad65; assay MGI:6296657) and Gad1 (Gad67; assay MGI:6295980).
|
Specimen
2a ISH Crh - upper:
postnatal week 10-14
(more )
Specimen
2a ISH Crh - upper:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
2a ISH Crh - lower:
postnatal week 10-14
(more )
Specimen
2a ISH Crh - lower:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
2a Camk2a + Crh - upper:
postnatal week 10-14
(more )
Note: Double labeled: silver grains - Crh; red - Camk2a (assay MGI:6295983)
Specimen
2a Camk2a + Crh - upper:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: silver grains - Crh; red - Camk2a (assay MGI:6295983)
|
Specimen
2a Camk2a + Crh - lower:
postnatal week 10-14
(more )
Note: Double labeled: silver grains - Crh; red - Camk2a (assay MGI:6295983)
Specimen
2a Camk2a + Crh - lower:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: silver grains - Crh; red - Camk2a (assay MGI:6295983)
|
Specimen
S2a ISH Crh - top:
postnatal week 10-14
(more )
Specimen
S2a ISH Crh - top:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: piriform cortex |
Present |
|
S2a left top
|
Expression is present in neurons.
|
Specimen
S2a ISH Crh - middle:
postnatal week 10-14
(more )
Specimen
S2a ISH Crh - middle:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
S2a ISH Crh - bottom:
postnatal week 10-14
(more )
Specimen
S2a ISH Crh - bottom:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
S2b Pir:
(close )
Genetic Background:
|
involves: 129S6/SvEvTac * C57BL/6
|
Mutant Allele(s):
|
Crhtm1(cre)Zjh/Crh+ Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Conditional mutant. Double labeled: silver grains - Crh; red - Cre reporter (to reveal neuron morphology.)
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: piriform cortex |
Present |
|
S2b Pir
|
Expression is present in neurons.
|
Specimen
S2b Ctx:
(close )
Genetic Background:
|
involves: 129S6/SvEvTac * C57BL/6
|
Mutant Allele(s):
|
Crhtm1(cre)Zjh/Crh+ Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Conditional mutant. Double labeled: silver grains - Crh; red - Cre reporter (to reveal neuron morphology.)
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cerebral cortex |
Present |
|
S2b Ctx
|
Expression is present in neurons.
|
Specimen
S2b Hip-CA1:
(close )
Genetic Background:
|
involves: 129S6/SvEvTac * C57BL/6
|
Mutant Allele(s):
|
Crhtm1(cre)Zjh/Crh+ Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Conditional mutant. Double labeled: silver grains - Crh; red - Cre reporter (to reveal neuron morphology.)
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: hippocampus CA1 |
Present |
|
S2b Hip-CA1
|
Expression is present in neurons.
|
Specimen
S2b CeA:
(close )
Genetic Background:
|
involves: 129S6/SvEvTac * C57BL/6
|
Mutant Allele(s):
|
Crhtm1(cre)Zjh/Crh+ Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Conditional mutant. Double labeled: silver grains - Crh; red - Cre reporter (to reveal neuron morphology.)
|
Specimen
S2b adBNST:
(close )
Genetic Background:
|
involves: 129S6/SvEvTac * C57BL/6
|
Mutant Allele(s):
|
Crhtm1(cre)Zjh/Crh+ Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Conditional mutant. Double labeled: silver grains - Crh; red - Cre reporter (to reveal neuron morphology.)
|
Specimen
S2b avBNST:
(close )
Genetic Background:
|
involves: 129S6/SvEvTac * C57BL/6
|
Mutant Allele(s):
|
Crhtm1(cre)Zjh/Crh+ Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Conditional mutant. Double labeled: silver grains - Crh; red - Cre reporter (to reveal neuron morphology.)
|
Specimen
S2b PVN:
(close )
Genetic Background:
|
involves: 129S6/SvEvTac * C57BL/6
|
Mutant Allele(s):
|
Crhtm1(cre)Zjh/Crh+ Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Conditional mutant. Double labeled: silver grains - Crh; red - Cre reporter (to reveal neuron morphology.)
|
Specimen
S3a ISH Crh - upper:
postnatal week 10-14
(more )
Specimen
S3a ISH Crh - upper:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: piriform cortex |
Present |
|
S3a ISH Crh - upper
|
Expression is present in neurons.
|
Specimen
S3a GAD65/67 + Crh - Pir:
postnatal week 10-14
(more )
Note: Double labeled: silver grains - Crh; red - Gad2 (Gad65; assay MGI:6296657) and Gad1 (Gad67; assay MGI:6295980).
Specimen
S3a GAD65/67 + Crh - Pir:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: silver grains - Crh; red - Gad2 (Gad65; assay MGI:6296657) and Gad1 (Gad67; assay MGI:6295980).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: piriform cortex |
Present |
|
S3a GAD65/67 + Crh - Pir
|
Expression is present in neurons; none co-express GAD65/67.
|
Specimen
S3a Vglut1 + Crh - Pir:
postnatal week 10-14
(more )
Note: Double labeled: silver grains - Crh; red - Slc17a7 (Vglut1; assay MGI:6295984)
Specimen
S3a Vglut1 + Crh - Pir:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: silver grains - Crh; red - Slc17a7 (Vglut1; assay MGI:6295984)
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: piriform cortex |
Present |
|
S3a Vglut1 + Crh - Pir
|
Expression is present in neurons; the majority co-express Vglut1.
|
Specimen
S3a Vglut2 + Crh - Pir:
postnatal week 10-14
(more )
Note: Double labeled: silver grains - Crh; red - Slc17a6 (Vglut2; assay MGI:6295986)
Specimen
S3a Vglut2 + Crh - Pir:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: silver grains - Crh; red - Slc17a6 (Vglut2; assay MGI:6295986)
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: piriform cortex |
Present |
|
S3a Vglut2 + Crh - Pir
|
Expression is present in neurons; a subset co-express Vglut2.
|
Specimen
S3a Camk2a + Crh - Pir:
postnatal week 10-14
(more )
Note: Double labeled: silver grains - Crh; red - Camk2a (assay MGI:6295983)
Specimen
S3a Camk2a + Crh - Pir:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: silver grains - Crh; red - Camk2a (assay MGI:6295983)
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: piriform cortex |
Present |
|
S3a Camk2a + Crh - Pir
|
Expression is present in neurons; the majority co-express Camk2a.
|
Specimen
S3a ISH Crh - 2nd down:
postnatal week 10-14
(more )
Specimen
S3a ISH Crh - 2nd down:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
S3a GAD65/67 + Crh - adBNST:
postnatal week 10-14
(more )
Note: Double labeled: silver grains - Crh; red - Gad2 (Gad65; assay MGI:6296657) and Gad1 (Gad67; assay MGI:6295980).
Specimen
S3a GAD65/67 + Crh - adBNST:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: silver grains - Crh; red - Gad2 (Gad65; assay MGI:6296657) and Gad1 (Gad67; assay MGI:6295980).
|
Specimen
S3a Vglut1 + Crh - adBNST:
postnatal week 10-14
(more )
Note: Double labeled: silver grains - Crh; red - Slc17a7 (Vglut1; assay MGI:6295984)
Specimen
S3a Vglut1 + Crh - adBNST:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: silver grains - Crh; red - Slc17a7 (Vglut1; assay MGI:6295984)
|
Specimen
S3a Vglut2 + Crh - adBNST:
postnatal week 10-14
(more )
Note: Double labeled: silver grains - Crh; red - Slc17a6 (Vglut2; assay MGI:6295986)
Specimen
S3a Vglut2 + Crh - adBNST:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: silver grains - Crh; red - Slc17a6 (Vglut2; assay MGI:6295986)
|
Specimen
S3a Camk2a + Crh - adBNST:
postnatal week 10-14
(more )
Note: Double labeled: silver grains - Crh; red - Camk2a (assay MGI:6295983)
Specimen
S3a Camk2a + Crh - adBNST:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: silver grains - Crh; red - Camk2a (assay MGI:6295983)
|
Specimen
S3a GAD65/67 + Crh - avBNST:
postnatal week 10-14
(more )
Note: Double labeled: silver grains - Crh; red - Gad2 (Gad65; assay MGI:6296657) and Gad1 (Gad67; assay MGI:6295980).
Specimen
S3a GAD65/67 + Crh - avBNST:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: silver grains - Crh; red - Gad2 (Gad65; assay MGI:6296657) and Gad1 (Gad67; assay MGI:6295980).
|
Specimen
S3a Vglut1 + Crh - avBNST:
postnatal week 10-14
(more )
Note: Double labeled: silver grains - Crh; red - Slc17a7 (Vglut1; assay MGI:6295984)
Specimen
S3a Vglut1 + Crh - avBNST:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: silver grains - Crh; red - Slc17a7 (Vglut1; assay MGI:6295984)
|
Specimen
S3a Vglut2 + Crh - avBNST:
postnatal week 10-14
(more )
Note: Double labeled: silver grains - Crh; red - Slc17a6 (Vglut2; assay MGI:6295986)
Specimen
S3a Vglut2 + Crh - avBNST:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: silver grains - Crh; red - Slc17a6 (Vglut2; assay MGI:6295986)
|
Specimen
S3a Camk2a + Crh - avBNST:
postnatal week 10-14
(more )
Note: Double labeled: silver grains - Crh; red - Camk2a (assay MGI:6295983)
Specimen
S3a Camk2a + Crh - avBNST:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: silver grains - Crh; red - Camk2a (assay MGI:6295983)
|
Specimen
S3a ISH Crh - 3rd down:
postnatal week 10-14
(more )
Specimen
S3a ISH Crh - 3rd down:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
S3a GAD65/67 + Crh - PVN:
postnatal week 10-14
(more )
Note: Double labeled: silver grains - Crh; red - Gad2 (Gad65; assay MGI:6296657) and Gad1 (Gad67; assay MGI:6295980).
Specimen
S3a GAD65/67 + Crh - PVN:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: silver grains - Crh; red - Gad2 (Gad65; assay MGI:6296657) and Gad1 (Gad67; assay MGI:6295980).
|
Specimen
S3a Vglut1 + Crh - PVN:
postnatal week 10-14
(more )
Note: Double labeled: silver grains - Crh; red - Slc17a7 (Vglut1; assay MGI:6295984)
Specimen
S3a Vglut1 + Crh - PVN:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: silver grains - Crh; red - Slc17a7 (Vglut1; assay MGI:6295984)
|
Specimen
S3a Vglut2 + Crh - PVN:
postnatal week 10-14
(more )
Note: Double labeled: silver grains - Crh; red - Slc17a6 (Vglut2; assay MGI:6295986)
Specimen
S3a Vglut2 + Crh - PVN:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: silver grains - Crh; red - Slc17a6 (Vglut2; assay MGI:6295986)
|
Specimen
S3a Camk2a + Crh - PVN:
postnatal week 10-14
(more )
Note: Double labeled: silver grains - Crh; red - Camk2a (assay MGI:6295983)
Specimen
S3a Camk2a + Crh - PVN:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: silver grains - Crh; red - Camk2a (assay MGI:6295983)
|
Specimen
S3a GAD65/67 + Crh - CeA:
postnatal week 10-14
(more )
Note: Double labeled: silver grains - Crh; red - Gad2 (Gad65; assay MGI:6296657) and Gad1 (Gad67; assay MGI:6295980).
Specimen
S3a GAD65/67 + Crh - CeA:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: silver grains - Crh; red - Gad2 (Gad65; assay MGI:6296657) and Gad1 (Gad67; assay MGI:6295980).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: central amygdaloid nucleus |
Present |
|
S3a GAD65/67 + Crh - CeA
|
Expression is present in neurons; the majority co-express GAD65/67.
|
Specimen
S3a Vglut1 + Crh - CeA:
postnatal week 10-14
(more )
Note: Double labeled: silver grains - Crh; red - Slc17a7 (Vglut1; assay MGI:6295984)
Specimen
S3a Vglut1 + Crh - CeA:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: silver grains - Crh; red - Slc17a7 (Vglut1; assay MGI:6295984)
|
Specimen
S3a Vglut2 + Crh - CeA:
postnatal week 10-14
(more )
Note: Double labeled: silver grains - Crh; red - Slc17a6 (Vglut2; assay MGI:6295986)
Specimen
S3a Vglut2 + Crh - CeA:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: silver grains - Crh; red - Slc17a6 (Vglut2; assay MGI:6295986)
|
Specimen
S3a Camk2a + Crh - CeA:
postnatal week 10-14
(more )
Note: Double labeled: silver grains - Crh; red - Camk2a (assay MGI:6295983)
Specimen
S3a Camk2a + Crh - CeA:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: silver grains - Crh; red - Camk2a (assay MGI:6295983)
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: central amygdaloid nucleus |
Present |
|
S3a Camk2a + Crh - CaE
|
Expression is present in neurons; a subset co-express Camk2a.
|
Specimen
S3a ISH Crh - lowest:
postnatal week 10-14
(more )
Specimen
S3a ISH Crh - lowest:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cerebral cortex |
Present |
|
S3a ISH Crh - lowest
|
Expression is present in neurons.
|
TS28: hippocampus |
Present |
|
S3a ISH Crh - lowest
|
Expression is present in neurons.
|
Specimen
S3a GAD65/67 + Crh - Ctx:
postnatal week 10-14
(more )
Note: Double labeled: silver grains - Crh; red - Gad2 (Gad65; assay MGI:6296657) and Gad1 (Gad67; assay MGI:6295980).
Specimen
S3a GAD65/67 + Crh - Ctx:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: silver grains - Crh; red - Gad2 (Gad65; assay MGI:6296657) and Gad1 (Gad67; assay MGI:6295980).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cerebral cortex |
Present |
|
S3a GAD65/67 + Crh - Ctx
|
Expression is present in neurons; the majority co-express GAD65/67.
|
Specimen
S3a Vglut1 + Crh - Ctx:
postnatal week 10-14
(more )
Note: Double labeled: silver grains - Crh; red - Slc17a7 (Vglut1; assay MGI:6295984)
Specimen
S3a Vglut1 + Crh - Ctx:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: silver grains - Crh; red - Slc17a7 (Vglut1; assay MGI:6295984)
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cerebral cortex |
Present |
|
S3a Vglut1 + Crh - CTX
|
Expression is present in neurons; a subset co-express Vglut1.
|
Specimen
S3a Vglut2 + Crh - Ctx:
postnatal week 10-14
(more )
Note: Double labeled: silver grains - Crh; red - Slc17a6 (Vglut2; assay MGI:6295986)
Specimen
S3a Vglut2 + Crh - Ctx:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: silver grains - Crh; red - Slc17a6 (Vglut2; assay MGI:6295986)
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cerebral cortex |
Present |
|
S3a Vglut2 + Crh - Ctx
|
Expression is present in neurons; a subset co-express Vglut2.
|
Specimen
S3a Camk2a + Crh - Ctx:
postnatal week 10-14
(more )
Note: Double labeled: silver grains - Crh; red - Camk2a (assay MGI:6295983)
Specimen
S3a Camk2a + Crh - Ctx:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: silver grains - Crh; red - Camk2a (assay MGI:6295983)
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cerebral cortex |
Present |
|
S3a Camk2a + Crh - Ctx
|
Expression is present in neurons; a subset co-express Camk2a.
|
Specimen
S3a GAD65/67 + Crh - Hip-CA1:
postnatal week 10-14
(more )
Note: Double labeled: silver grains - Crh; red - Gad2 (Gad65; assay MGI:6296657) and Gad1 (Gad67; assay MGI:6295980).
Specimen
S3a GAD65/67 + Crh - Hip-CA1:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: silver grains - Crh; red - Gad2 (Gad65; assay MGI:6296657) and Gad1 (Gad67; assay MGI:6295980).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: hippocampus CA1 |
Present |
|
S3a GAD65/67 + Crh - Hip-CA1
|
Expression is present in neurons; the majority co-express GAD65/67.
|
Specimen
S3a Vglut1 + Crh - Hip-CA1:
postnatal week 10-14
(more )
Note: Double labeled: silver grains - Crh; red - Slc17a7 (Vglut1; assay MGI:6295984)
Specimen
S3a Vglut1 + Crh - Hip-CA1:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: silver grains - Crh; red - Slc17a7 (Vglut1; assay MGI:6295984)
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: hippocampus CA1 |
Present |
|
S3a Vglut1 + Crh - Hip-CA1
|
Expression is present in neurons; a subset co-express Vglut1.
|
Specimen
S3a Vglut2 + Crh - Hip-CA1:
postnatal week 10-14
(more )
Note: Double labeled: silver grains - Crh; red - Slc17a6 (Vglut2; assay MGI:6295986)
Specimen
S3a Vglut2 + Crh - Hip-CA1:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: silver grains - Crh; red - Slc17a6 (Vglut2; assay MGI:6295986)
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: hippocampus CA1 |
Present |
|
S3a Vglut2 + Crh - Hip-CA1
|
Expression is present in neurons; none co-express Vglut2.
|
Specimen
S3a Camk2a + Crh - Hip-CA1:
postnatal week 10-14
(more )
Note: Double labeled: silver grains - Crh; red - Camk2a (assay MGI:6295983)
Specimen
S3a Camk2a + Crh - Hip-CA1:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: silver grains - Crh; red - Camk2a (assay MGI:6295983)
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: hippocampus CA1 |
Present |
|
S3a Camk2a + Crh - Hip-CA1
|
Expression is present in neurons; none co-express Camk2a.
|
Specimen
S4a Crh + Pkcd - left:
postnatal week 10-14
(more )
Note: Double labeled: silver grains - Crh; red - Prkcd (assay MGI:6295988)
Specimen
S4a Crh + Pkcd - left:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: silver grains - Crh; red - Prkcd (assay MGI:6295988)
|
Specimen
S4a Crh + Pkcd - center:
postnatal week 10-14
(more )
Note: Double labeled: silver grains - Crh; red - Prkcd (assay MGI:6295988)
Specimen
S4a Crh + Pkcd - center:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: silver grains - Crh; red - Prkcd (assay MGI:6295988)
|
Specimen
S4a Crh + Pkcd - right:
postnatal week 10-14
(more )
Note: Double labeled: silver grains - Crh; red - Prkcd (assay MGI:6295988)
Specimen
S4a Crh + Pkcd - right:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: silver grains - Crh; red - Prkcd (assay MGI:6295988)
|
Specimen
S4a Crh + Som - left:
postnatal week 10-14
(more )
Note: Double labeled: silver grains - Crh; red - Sst (assay MGI:6295989)
Specimen
S4a Crh + Som - left:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: silver grains - Crh; red - Sst (assay MGI:6295989)
|
Specimen
S4a Crh + Som - center:
postnatal week 10-14
(more )
Note: Double labeled: silver grains - Crh; red - Sst (assay MGI:6295989)
Specimen
S4a Crh + Som - center:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: silver grains - Crh; red - Sst (assay MGI:6295989)
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: central amygdaloid nucleus |
Present |
|
S4a lower center
|
Expression is present in neurons; a subset co-express somatostatin.
|
Specimen
S4a Crh + Som - right:
postnatal week 10-14
(more )
Note: Double labeled: silver grains - Crh; red - Sst (assay MGI:6295989)
Specimen
S4a Crh + Som - right:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: silver grains - Crh; red - Sst (assay MGI:6295989)
|
Specimen
S7a fl/fl 1st:
(close )
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6J
|
Mutant Allele(s):
|
Crhtm1.1Jde/Crhtm1.1Jde
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
S7a fl/fl 2nd:
(close )
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6J
|
Mutant Allele(s):
|
Crhtm1.1Jde/Crhtm1.1Jde
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
S7a fl/fl 3rd:
(close )
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6J
|
Mutant Allele(s):
|
Crhtm1.1Jde/Crhtm1.1Jde
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
S7a fl/fl 4th:
(close )
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6J
|
Mutant Allele(s):
|
Crhtm1.1Jde/Crhtm1.1Jde
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
S7a fl/fl 5th:
(close )
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6J
|
Mutant Allele(s):
|
Crhtm1.1Jde/Crhtm1.1Jde
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: brain |
Present |
|
S7f fl/fl 5th
|
|
Specimen
S7a -/- 1st:
(close )
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6J
|
Mutant Allele(s):
|
Crhtm1.2Jde/Crhtm1.2Jde
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
S7a -/- 2nd:
(close )
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6J
|
Mutant Allele(s):
|
Crhtm1.2Jde/Crhtm1.2Jde
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
S7a -/- 3rd:
(close )
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6J
|
Mutant Allele(s):
|
Crhtm1.2Jde/Crhtm1.2Jde
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
S7a -/- 4th:
(close )
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6J
|
Mutant Allele(s):
|
Crhtm1.2Jde/Crhtm1.2Jde
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
S7a -/- 5th:
(close )
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6J
|
Mutant Allele(s):
|
Crhtm1.2Jde/Crhtm1.2Jde
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: brain |
Absent |
|
S7f -/- 5th
|
|
Specimen
S9a Ctrl - left:
(close )
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6J
|
Mutant Allele(s):
|
Crhtm1.1Jde/Crhtm1.1Jde
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
S9a Ctrl - center:
(close )
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6J
|
Mutant Allele(s):
|
Crhtm1.1Jde/Crhtm1.1Jde
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
S9a Ctrl - right:
(close )
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6J
|
Mutant Allele(s):
|
Crhtm1.1Jde/Crhtm1.1Jde
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
S9a CKO - left:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ * C57BL/6J
|
Mutant Allele(s):
|
Crhtm1.1Jde/Crhtm1.1Jde Neurod6tm1(cre)Kan/Neurod6+
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Conditional mutant.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: piriform cortex |
Trace |
|
S9a lower left
|
Glutamatergic neurons have been eliminated in the mutant.
|
Specimen
S9a CKO - center:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ * C57BL/6J
|
Mutant Allele(s):
|
Crhtm1.1Jde/Crhtm1.1Jde Neurod6tm1(cre)Kan/Neurod6+
|
Age:
|
postnatal week 10-14
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Conditional mutant.
|
Specimen
S9a CKO - right:
(close )
Genetic Background:
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involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Crhtm1.1Jde/Crhtm1.1Jde Neurod6tm1(cre)Kan/Neurod6+
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Age:
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postnatal week 10-14
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Sex:
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Male
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Conditional mutant.
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Structure |
Level |
Pattern |
Image |
Note |
TS28: central amygdaloid nucleus |
Present |
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S9a lower right
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Expression in the mutant is essentially the same as seen in wild type.
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