About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:217679 Washkowitz AJ, et al., Mga is essential for the survival of pluripotent cells during peri-implantation development. Development. 2015 Jan 1;142(1):31-40
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6258283
Gene symbol: Pou5f1
Gene name: POU domain, class 5, transcription factor 1
Reporter: GFP
Antibody: anti-GFP (#A11122)
Detection system: Secondary antibody coupled to fluorescein
Results
Specimen 5 WT: embryonic day 4.5; MgaGt(E153E01)Wrst/Mga+, Pou5f1tm2Jae/Pou5f1+ (more )
Note: Embryos were either Mga+/+ or MgaGt/+. Double labeled: green - Pou5f1 reporter; red - Gata4 (assay MGI:6258231).
Structure Level Pattern Image Note
TS6: epiblast Present 5_WT OctGFP/Gata4
Specimen 5 MgaGt/Gt: embryonic day 4.5; MgaGt(E153E01)Wrst/MgaGt(E153E01)Wrst, Pou5f1tm2Jae/Pou5f1+ (more )
Note: Double labeled: green - Pou5f1 reporter; red - Gata4 (assay MGI:6258231).
Structure Level Pattern Image Note
TS6: epiblast Present 5_MgaGt/Gt OctGFP/Gata4 Expression in this mutant is the same as in wild-type embryos.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/18/2025
MGI 6.24 		The Jackson Laboratory