Gata4 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:217679 Washkowitz AJ, et al., Mga is essential for the survival of pluripotent cells during peri-implantation development. Development. 2015 Jan 1;142(1):31-40
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6258231
Gene symbol:	Gata4
Gene name:	GATA binding protein 4

Antibody:	anti-GATA-4 (sc-1237)
Detection system:	Secondary antibody

Results

Specimen 5 WT Nanog/Gat4: embryonic day 4.5; Mga^{Gt(E153E01)Wrst}/Mga⁺ (more

)
Note: Embryos were either Mga+/+ or MgaGt/+. Double labeled: red - Gata4; green - Nanog (assay MGI:6258230).

Structure	Level	Pattern	Image	Note
TS6: primitive endoderm	Present		5_WT Nanog/Gata4

Specimen 5 MgaGt/Gt Nanog/Gata4: embryonic day 4.5; Mga^{Gt(E153E01)Wrst}/Mga^{Gt(E153E01)Wrst} (more

)
Note: Double labeled: red - Gata4; green - Nanog (assay MGI:6258230).

Structure	Level	Pattern	Image	Note
TS6: primitive endoderm	Present		5_MgaGt/GT Nanog/Gata4	Expression in this mutant is the same as in wild-type embryos.

Specimen 5 WT OctGFP/Gata4: embryonic day 4.5; Mga^{Gt(E153E01)Wrst}/Mga⁺, Pou5f1^tm2Jae/Pou5f1⁺ (more

)
Note: Embryos were either Mga+/+ or MgaGt/+. Double labeled: red - Gata4; green - Pou5f1 reporter (assay MGI:6258283).

Structure	Level	Pattern	Image	Note
TS6: primitive endoderm	Present		5_WT OctGFP/Gata4

Specimen 5 MgaGt/Gt OctGFP/Gata4: embryonic day 4.5; Mga^{Gt(E153E01)Wrst}/Mga^{Gt(E153E01)Wrst}, Pou5f1^tm2Jae/Pou5f1⁺ (more

)
Note: Double labeled: red - Gata4; green - Pou5f1 reporter (assay MGI:6258283).

Structure	Level	Pattern	Image	Note
TS6: primitive endoderm	Present		5_MgaGt/Gt OctGFP/Gata4	Expression in this mutant is the same as in wild-type embryos.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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