Antibody:

Dnmt3L antibody

Structure	Level	Pattern	Image	Note
TS28: oocyte	Present		1A Oocyte	Expression was detected on meiotic chromosomes of the ovulated oocyte.

Structure	Level	Pattern	Image	Note
TS1: 1-cell stage embryo	Present		1A 1-cell	The maternal protein was distributed equally on the maternal and paternal pronuclei, with enrichment in DAPI-dense ring structures around nucleolar bodies.

Structure	Level	Pattern	Image	Note
TS2: 2-cell stage embryo	Present		1A 2-cell	Expression was detected in the nucleus.

Structure	Level	Pattern	Image	Note
TS3: 4-cell stage embryo	Absent		1A 4-cell

Structure	Level	Pattern	Image	Note
TS3: compacted morula	Absent		1A E2.5

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Present		1A E3.5	Expression was detected in all the blastomeres of the blastocyst. Cell-to-cell variation in intensity was noted but no obvious delineation between the inner cell mass and the trophectoderm.
TS4: inner cell mass	Present		1A E3.5
TS4: trophectoderm	Present		1A E3.5

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Present		1A E4.5
TS5: trophectoderm	Absent		1A E4.5

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Present		1B E3.5 merge , 1B E3.5	Expression was detected in all the blastomeres of the blastocyst. Cell-to-cell variation in intensity was noted but no obvious delineation between the inner cell mass and the trophectoderm.
TS4: inner cell mass	Present		1B E3.5 merge , 1B E3.5
TS4: trophectoderm	Present		1B E3.5 merge , 1B E3.5

Structure	Level	Pattern	Image	Note
TS5: blastocyst	Present	Regionally restricted	1B E4.25 , 1B E4.25 merge	Expression progressively disappears from the mural and the polar trophectoderm (marked by Cdx2), and is instead specific to the inner cell mass.
TS5: inner cell mass	Present		1B E4.25 merge , 1B E4.25
TS5: mural trophectoderm	Absent		1B E4.25 , 1B E4.25 merge
TS5: polar trophectoderm	Absent		1B E4.25 , 1B E4.25 merge

Structure	Level	Pattern	Image	Note
TS5: blastocyst	Present	Regionally restricted	1B E4.5 , 1B E4.5 merge	Expression progressively disappears from the mural and the polar trophectoderm (marked by Cdx2), and is instead specific to the inner cell mass.
TS5: inner cell mass	Present		1B E4.5 , 1B E4.5 merge
TS5: mural trophectoderm	Absent		1B E4.5 , 1B E4.5 merge
TS5: polar trophectoderm	Absent		1B E4.5 , 1B E4.5 merge

Structure	Level	Pattern	Image	Note
TS1: 1-cell stage embryo	Present		S1_top panel

Structure	Level	Pattern	Image	Note
TS1: 1-cell stage embryo	Absent		S1_lower panel

Structure	Level	Pattern	Image	Note
TS6: blastocyst	Present		S2A E4 Dnmt3L , S2A E4 merge
TS6: primitive endoderm	Present		S2A E4 Dnmt3L , S2A E4 merge

Structure	Level	Pattern	Image	Note
TS6: blastocyst	Present		S2A E4.25 merge , S2A E4.25 Dnmt3L
TS6: primitive endoderm	Present		S2A E4.25 merge , S2A E4.25 Dnmt3L