Gene Expression Data
Assay Details
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Assay
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Reference:
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J:130836
Gerbe F, et al., Dynamic expression of Lrp2 pathway members reveals progressive epithelial differentiation of primitive endoderm in mouse blastocyst. Dev Biol. 2008 Jan 15;313(2):594-602
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6189096
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Gene symbol:
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Nanog
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Gene name:
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Nanog homeobox
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Antibody:
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anti-Nanog
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Assay notes:
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Two antibodies were described, but the one used in this assay was not detailed. Anti-Nanog MGI:6189070 may have been used in this assay.
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Results
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Specimen
4A:
embryonic day 3.5
(more  )
Note: 24 cells. Triple labeled: green - Nanog; red - Lrp2 (assay MGI:6189094); white either Cdh1 (assay MGI:6189097) or Hoechst.
Specimen
4A:
(close  )
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Genetic Background:
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either: CD-1 or ICR
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Age:
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embryonic day 3.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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24 cells. Triple labeled: green - Nanog; red - Lrp2 (assay MGI:6189094); white either Cdh1 (assay MGI:6189097) or Hoechst.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS4: inner cell mass |
Present |
Regionally restricted |
4A Nanog
,
4A E-Cadh
,
4A Hoechst
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Authors report exclusive expression in inner cell mass cells at every time point analyzed, and that the propotion of expressing cells ermains nearly the same.
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Specimen
4B:
embryonic day 3.75
(more )
Note: 26 cells. Triple labeled: green - Nanog; red - Lrp2 (assay MGI:6189094); white either Cdh1 (assay MGI:6189097) or Hoechst.
Specimen
4B:
(close )
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Genetic Background:
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either: CD-1 or ICR
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Age:
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embryonic day 3.75
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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26 cells. Triple labeled: green - Nanog; red - Lrp2 (assay MGI:6189094); white either Cdh1 (assay MGI:6189097) or Hoechst.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS5: inner cell mass |
Present |
Regionally restricted |
4B Hoechst
,
4B Nanog
,
4B E-Cadh
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Authors report exclusive expression in inner cell mass cells at every time point analyzed, and that the propotion of expressing cells ermains nearly the same.
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Specimen
4C:
embryonic day 3.75
(more )
Note: 27 cells. Triple labeled: green - Nanog; red - Lrp2 (assay MGI:6189094); white either Cdh1 (assay MGI:6189097) or Hoechst.
Specimen
4C:
(close )
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Genetic Background:
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either: CD-1 or ICR
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Age:
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embryonic day 3.75
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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27 cells. Triple labeled: green - Nanog; red - Lrp2 (assay MGI:6189094); white either Cdh1 (assay MGI:6189097) or Hoechst.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS4: inner cell mass |
Present |
Regionally restricted |
4C Nanog
,
4C E-Cadh
,
4C Hoechst
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Authors report exclusive expression in inner cell mass cells at every time point analyzed, and that the propotion of expressing cells ermains nearly the same.
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Analysis Tools