Antibody:

anti-Megalin

Structure	Level	Pattern	Image	Note
TS6: primitive endoderm	Present	Regionally restricted	2D	Strong expression in apical part of primitive endoderm cells. Authors report expression is strongly polarized in the epithelium at E4.5. Localization corresponds to future apical side.

Structure	Level	Pattern	Image	Note
TS4: trophectoderm	Strong	Widespread	3A	Expression in trophoblasts. Authors state all trophoblast cells are strongly stained.
TS4: inner cell mass	Weak	Regionally restricted	3A	Weak expression in only one or two cells in the inner cell mass, in a "salt-and-pepper" pattern - some cells facing the blastocoelic cavity, in contact or not with trophoblasts. In younger embryos, expression homogenously distributed in the cytoplasm around the nucleus.

Structure	Level	Pattern	Image	Note
TS4: trophectoderm	Strong	Widespread	3B	Expression in trophoblasts. Authors state all trophoblast cells are strongly stained.
TS4: inner cell mass	Weak	Regionally restricted	3B	Weak expression in only one or two cells in the Inner cell mass, in a "salt-and-pepper" pattern - some cells localized deeper in the inner cell mass. In cells not located at the blastocoelic surface, expression homogenously distributed in the cytoplasm around the nucleus.

Structure	Level	Pattern	Image	Note
TS4: trophectoderm	Strong	Widespread	3C	Expression in trophoblasts. Authors state all trophoblast cells are strongly stained.
TS4: inner cell mass	Weak	Regionally restricted	3C	Weak expression in only one or two cells in the Inner cell mass, in a "salt-and-pepper" pattern - some cells facing the blastocoelic cavity, in contact or not with trophoblasts. In cells not located at the blastocoelic surface, expression homogenously distributed in the cytoplasm around the nucleus.

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present	Punctate	3C'	Localization of expression appeared punctate and was not associated with the plasma membrane.

Structure	Level	Pattern	Image	Note
TS4: trophectoderm	Strong	Widespread	3D	Expression in trophoblasts. Authors state all trophoblast cells are strongly stained.
TS4: inner cell mass	Present	Regionally restricted	3D	Authors report more expressing cells in older E3.75 blastocysts.

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present	Regionally restricted	4A LRP2 , 4A Hoechst , 4A E-Cadh	Authors report exclusive expression in inner cell mass cells at every time point analyzed.

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Present	Regionally restricted	4B LRP2 , 4B Hoechst , 4B E-Cadh	Authors state the number of expressing cells in the inner cell mass almost doubled during blastocyst maturation.

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Present	Regionally restricted	4C Hoechst , 4C LRP2 , 4C E-Cadh	Authors state the number of expressing cells in the inner cell mass almost doubled during blastocyst maturation.

Structure	Level	Pattern	Image	Note
TS5: blastocyst	Present	Regionally restricted	5A MERGE , 5A LRP2	Polarized expression in E3.75 embryos only in pre-primitive endoderm cells that had lost contact with the blastocoelic cavity. Localization corresponds to future apical side. Authors report strong increase in level of expresion.

Structure	Level	Pattern	Image	Note
TS6: blastocyst	Present	Regionally restricted	5B MERGE , 5B LRP2	Authors report expression is strongly polarized in the epithelium at E4.5. Localization corresponds to future apical side.

Structure	Level	Pattern	Image	Note
TS6: blastocyst	Present	Regionally restricted	5C MERGE , 5C LRP2	Authors report expression is strongly polarized in the epithelium at E4.5. Localization corresponds to future apical side.

Structure	Level	Pattern	Image	Note
TS6: blastocyst	Present		S1A Merge , S1A LRP2