Gene Expression Data
Assay Details
Assay
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Reference:
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J:257654
Memic F, et al., Transcription and Signaling Regulators in Developing Neuronal Subtypes of Mouse and Human Enteric Nervous System. Gastroenterology. 2018 Feb;154(3):624-636
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6189075
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Gene symbol:
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Npy
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Gene name:
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neuropeptide Y
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Antibody:
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anti-NPY (ab6173)
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Detection system:
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Secondary antibody
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Assay notes:
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The authors used two antibodies to NPY but did not distinguish which data was derived from which; it is likely MGI:6159145 was used to obtain at least some of these data.
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Results
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Specimen
Table 3A/B:
embryonic day 18.5
(more )
Specimen
Table 3A/B:
(close )
Genetic Background:
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either: C57BL/6 or MF1
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS26: enteric nervous system |
Present |
|
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Co-expression analysis was done (in the stomach) with a number of transcription factors. The table details which were co-expressed, partially co-expressed or not co-expressed.
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Specimen
4B:
embryonic day 18.5
(more )
Note: Double labeled: green - Npy; red - Sox6 (assay MGI:6188909).
Specimen
4B:
(close )
Genetic Background:
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either: C57BL/6 or MF1
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Npy; red - Sox6 (assay MGI:6188909).
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Specimen
4D - left:
embryonic day 18.5
(more )
Note: Double labeled: green - Npy; red - Calb1 (assay MGI:6189071).
Specimen
4D - left:
(close )
Genetic Background:
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either: C57BL/6 or MF1
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Npy; red - Calb1 (assay MGI:6189071).
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Structure |
Level |
Pattern |
Image |
Note |
TS26: enteric nervous system |
Present |
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4D NPY/CALB1
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Double staining shows 95% of NPY-positive neurons co-express Calb1.
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Specimen
4D - center:
embryonic day 18.5
(more )
Note: Double labeled: red - Npy; green - Th (assay MGI:6188917).
Specimen
4D - center:
(close )
Genetic Background:
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either: C57BL/6 or MF1
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Npy; green - Th (assay MGI:6188917).
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Specimen
4F:
embryonic day 18.5
(more )
Note: Littermates were used as controls.
Specimen
4F:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Littermates were used as controls.
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Specimen
4G:
(close )
Genetic Background:
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involves: 129 * C57BL/6J * CBA/J
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Mutant Allele(s):
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H2az2Tg(Wnt1-cre)11Rth/H2az2+ Sox6tm2.1Vlf/Sox6tm2.1Vlf
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Conditional mutant.
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Structure |
Level |
Pattern |
Image |
Note |
TS26: enteric nervous system |
Present |
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4G NPY
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Expression is present in gastric neurons; similar numbers are seen in mutant compared to wild type.
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