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Gene Expression Data
Assay Details
Assay
Reference: J:101265 Kuijper S, et al., Function and regulation of Alx4 in limb development: complex genetic interactions with Gli3 and Shh. Dev Biol. 2005 Sep 15;285(2):533-44
Assay type: RNA in situ
MGI Accession ID: MGI:6149012
Gene symbol: Grem1
Gene name: gremlin 1, DAN family BMP antagonist
Probe: Grem1 probe
Probe preparation: Antisense labelled with digoxigenin RNA
Results
Specimen 2A: embryonic day 10.5 (more )
Note: Also stained for Fgf4 (assay MGI:6149011).
Structure Level Pattern Image Note
TS17: forelimb bud mesenchyme Present Regionally restricted 2A Authors state that normal Grem1 expression is in posterior part of distal limb mesenchyme, close to Fgf4 expression in apical ectodermal ridge.

Specimen 2B: embryonic day 10.5; Alx4Lst-2J/Alx4lst-J (more )
Note: Also stained for Fgf4 (assay MGI:6149011).
Structure Level Pattern Image Note
TS17: forelimb bud Present Regionally restricted 2B Grem1 expression is upregulated in anterior part of limb bud.

Specimen 2C: embryonic day 10.5; Shhtm1Chg/Shhtm1Chg (more )
Note: Also stained for Fgf4 (assay MGI:6149011).
Structure Level Pattern Image Note
TS17: forelimb bud Weak Regionally restricted 2C Low levels of Grem1 expression in posterior part of limb bud.

Specimen 2C': embryonic day 10.5; Shhtm1Chg/Shhtm1Chg (more )
Note: Also stained for Fgf4 (assay MGI:6149011).
Structure Level Pattern Image Note
TS17: forelimb bud Weak Regionally restricted 2C' Low levels of Grem1 expression in posterior part of limb bud.

Specimen 2D: embryonic day 10.5; Alx4lst-J/Alx4lst-J, Shhtm1Chg/Shhtm1Chg (more )
Note: Also stained for Fgf4 (assay MGI:6149011).
Structure Level Pattern Image Note
TS17: forelimb bud Weak Regionally restricted 2D Expression reduced posteriorly in limb bud,

Specimen 2D': embryonic day 10.5; Alx4lst-J/Alx4lst-J, Shhtm1Chg/Shhtm1Chg (more )
Note: Also stained for Fgf4 (assay MGI:6149011).
Structure Level Pattern Image Note
TS17: forelimb bud Weak Regionally restricted 2D' Expression reduced posteriorly in limb bud,

Specimen 2E: embryonic day 10.5 (more )
Note: Also stained for Fgf4 (assay MGI:6149011).
Structure Level Pattern Image Note
TS17: hindlimb bud Present 2E

Specimen 2F: embryonic day 10.5; Alx4Lst-2J/Alx4lst-J (more )
Note: Also stained for Fgf4 (assay MGI:6149011).
Structure Level Pattern Image Note
TS17: hindlimb bud Present Regionally restricted 2F Grem1 expression is upregulated in anterior part of limb bud.

Specimen 2G: embryonic day 10.5; Shhtm1Chg/Shhtm1Chg (more )
Note: Also stained for Fgf4 (assay MGI:6149011).
Structure Level Pattern Image Note
TS17: hindlimb bud Present 2G

Specimen 2H: embryonic day 10.5; Alx4lst-J/Alx4lst-J, Shhtm1Chg/Shhtm1Chg (more )
Note: Also stained for Fgf4 (assay MGI:6149011).
Structure Level Pattern Image Note
TS17: hindlimb bud Present 2H Authors state Gremlin is not upregulated or exrpessd ectopically.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/07/2024
MGI 6.23
The Jackson Laboratory