Probe:	Glcci1 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS20: lateral ganglionic eminence	Present	Scattered	1A
TS20: medial ganglionic eminence	Present	Widespread	1A

Structure	Level	Pattern	Image	Note
TS21: medial ganglionic eminence	Present	Regionally restricted	1B	Expression was detected in the ventricular zone of the medial ganglionic eminence.
TS21: lateral ganglionic eminence	Present	Graded	1B	Robust expression was noted in a high dorsal to low ventral gradient in the lateral ganglionic eminence. Weak or absent expression was in the ventral most region of the ventral lateral ganglionic eminence.

Structure	Level	Pattern	Image	Note
TS26: medial ganglionic eminence	Present		1C
TS26: lateral ganglionic eminence	Present	Regionally restricted	1C	At E18.5, the expression gradient becomes restricted and largely confined to the dorsal lateral ganglionic eminence. Weak or absent expression was in the ventral most region of the ventral lateral ganglionic eminence.

Structure	Level	Pattern	Image	Note
TS28: lateral ganglionic eminence	Present	Regionally restricted	1D	Expression was restricted to the dorso-lateral subventricular zone region. Weak or absent expression was in the ventral most region of the ventral lateral ganglionic eminence.

Structure	Level	Pattern	Image	Note
TS24: main olfactory bulb	Present	Single cells	1E	Very few positively stained cells were detected in rostral olfactory bulb levels.
TS24: olfactory epithelium	Present		1E

Structure	Level	Pattern	Image	Note
TS24: main olfactory bulb	Present	Regionally restricted	1F	The strongest expression was observed in the ventral region of the olfactory bulb at caudal levels.

Structure	Level	Pattern	Image	Note
TS26: main olfactory bulb	Present	Regionally restricted		Very few positively stained cells were detected in rostral olfactory bulb levels. The strongest expression was observed in the ventral region of the olfactory bulb at caudal levels.

Structure	Level	Pattern	Image	Note
TS24: lateral ganglionic eminence	Present	Graded	1G	Robust expression was noted in a high dorsal to low ventral gradient in the lateral ganglionic eminence.
TS24: cerebral cortex	Present		1G
TS24: telencephalon septum	Present	Regionally restricted	1G	Expression was detected in the dorsal septum at rostral levels.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex	Present		1H
TS24: medial ganglionic eminence	Present		1H
TS24: lateral ganglionic eminence	Present	Graded	1H	Robust expression was noted in a high dorsal to low ventral gradient in the lateral ganglionic eminence. Weak or absent expression was in the ventral most region of the ventral lateral ganglionic eminence.

Structure	Level	Pattern	Image	Note
TS21: lateral ganglionic eminence	Present		2A
TS21: medial ganglionic eminence	Present		2A

Structure	Level	Pattern	Image	Note
TS21: lateral ganglionic eminence	Absent		2B
TS21: medial ganglionic eminence	Present		2B	There was no change in staining in the medial ganglionic eminence of the mutant.

Structure	Level	Pattern	Image	Note
TS26: lateral ganglionic eminence	Present		2E

Structure	Level	Pattern	Image	Note
TS26: lateral ganglionic eminence	Weak		2F

Structure	Level	Pattern	Image	Note
TS24: lateral ganglionic eminence	Present	Graded	4A , 4B	Graded expression enriched in the dorsal lateral ganglionic eminence. There was overlap of Glcci1 in situ signal in the Gsx2 immunopositive domain of the lateral ganglionic eminence. Overlap of expression was in the basal half of the ventricular zone near the ventricular zone/subventricular zone boundary.

Structure	Level	Pattern	Image	Note
TS24: lateral ganglionic eminence	Present	Graded	4C	Graded expression enriched in the dorsal lateral ganglionic eminence. There was minimal overlap with Sp8 immunostaining.