Antibody:	anti-Prdx6
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Weak	Uniform	8A	Relatively low and homogeneous expression.

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Weak	Uniform	8B	Relatively low and homogeneous expression.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Weak	Uniform	8C	Relatively low and homogeneous expression.

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Strong	Regionally restricted	8D	Strong ventral expression in ventral lateral cell population from E13.5 onward. Moderate expression in vascular tissue localized within spinal cord.
TS21: dorsal root ganglion	Present		8D
TS21: spinal cord meninges	Present		8D

Structure	Level	Pattern	Image	Note
TS22: spinal cord	Strong	Regionally restricted	8E	Strong ventral expression in ventral lateral cell population from E13.5 onward. Moderate expression in vascular tissue localized within spinal cord.
TS22: dorsal root ganglion	Present		8E
TS22: spinal cord meninges	Present		8E

Structure	Level	Pattern	Image	Note
TS24: spinal cord	Strong	Regionally restricted	8F	Strong ventral expression in ventral lateral cell population from E13.5 onward. Moderate expression in vascular tissue localized within spinal cord.
TS24: dorsal root ganglion	Present		8F
TS24: spinal cord meninges	Present		8F

Structure	Level	Pattern	Image	Note
TS26: spinal cord	Strong	Regionally restricted	8G	Strong ventral expression in ventral lateral cell population from E13.5 onward. Moderate expression in vascular tissue localized within spinal cord.
TS26: dorsal root ganglion	Present		8G
TS26: spinal cord meninges	Present		8G

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Strong	Regionally restricted	8H , 8K	Authors report strong ventral expression co-localized in nucleus with motor neuron markers Isl1/2 and Foxp1. Mainly nuclear and cytosolic localization of expression.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Strong	Regionally restricted	8L , 8O	Authors report strong ventral expression co-localized in nucleus with motor neuron markers Isl1/2 and Foxp1. Mainly nuclear and cytosolic localization of expression.

Structure	Level	Pattern	Image	Note
TS24: spinal cord	Strong	Regionally restricted	8P , 8S	Authors report strong ventral expression co-localized in nucleus with motor neuron markers Isl1/2 and Foxp1. Mainly nuclear and cytosolic localization of expression.

Structure	Level	Pattern	Image	Note
TS24: spinal cord	Strong	Regionally restricted	8T , 8W	Authors report strong ventral expression co-localized in nucleus with motor neuron markers Isl1/2 and Foxp1. Mainly nuclear and cytosolic localization of expression.