Gene Expression Data
Assay Details
Assay
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Reference:
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J:88779
Martin DM, et al., PITX2 is required for normal development of neurons in the mouse subthalamic nucleus and midbrain. Dev Biol. 2004 Mar 1;267(1):93-108
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6118172
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Gene symbol:
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Pitx2
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Gene name:
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paired-like homeodomain transcription factor 2
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Antibody:
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P2R10
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Detection system:
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Secondary antibody coupled to rhodamine
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Results
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Specimen
5D/F:
embryonic day 14.5
(more )
Note: Double labeled: red - Pitx2; green - Rbfox3 (assay MGI:6118173),
Specimen
5D/F:
(close )
Genetic Background:
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involves: C57BL/6J
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: red - Pitx2; green - Rbfox3 (assay MGI:6118173),
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: prethalamus |
Present |
Regionally restricted |
5D
,
5F
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Expression in neurons comprising the majority of cells in the subthalamic nucleus.
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Specimen
5G/I:
embryonic day 14.5; Pitx2tm2Sac/ Pitx2tm2Sac
(more )
Note: Double labeled: red - Pitx2; blue - DAPI.
Specimen
5G/I:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Pitx2tm2Sac/Pitx2tm2Sac
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
|
Double labeled: red - Pitx2; blue - DAPI.
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: prethalamus |
Present |
Regionally restricted |
5G
,
5I
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Expression in neurons comprising the majority of cells in the subthalamic nucleus.
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Specimen
5J/L:
embryonic day 14.5
(more )
Note: Double labeled: red - Pitx2; green - Calb2 (assay MGI:6118171).
Specimen
5J/L:
(close )
Genetic Background:
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involves: C57BL/6J
|
Age:
|
embryonic day 14.5
|
Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: red - Pitx2; green - Calb2 (assay MGI:6118171).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: prethalamus |
Present |
Regionally restricted |
5L
,
5J
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Expression in neurons comprising the majority of cells in the subthalamic nucleus.
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Specimen
5M/O:
embryonic day 14.5; Pitx2tm2Sac/ Pitx2tm2Sac
(more )
Note: Double labeled: red - Pitx2; green - BrdU.
Specimen
5M/O:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Pitx2tm2Sac/Pitx2tm2Sac
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
Embedding:
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Paraffin
|
Note:
|
Double labeled: red - Pitx2; green - BrdU.
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: prethalamus |
Present |
Regionally restricted |
5O
,
5M
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Expression in neurons comprising the majority of cells in the subthalamic nucleus.
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