Reporter:	lacZ
	Direct Detection
Assay notes:	Studies were conducted in Adamts5-/- mice for optimal staining. Adamts5+/- mice had identical staining patterns.

Structure	Level	Pattern	Image	Note
TS23: foot	Present		1C right
TS23: hindlimb interdigital region mesenchyme	Present		1C right
TS23: foot cartilage condensation	Absent		1C right

Structure	Level	Pattern	Image	Note
TS15: floor plate	Present		2A

Structure	Level	Pattern	Image	Note
TS15: floor plate	Present		2A'

Structure	Level	Pattern	Image	Note
TS19: face	Present	Regionally restricted	2B	Expression was detected in the developing muscles in the craniofacial region.
TS19: head	Present	Regionally restricted	2B	Expression was detected in the developing muscles in the craniofacial region.
TS19: peripheral nerve	Present	Regionally restricted	2B	Expression was detected in the peripheral nerves in the forelimb.
TS19: forelimb bud	Present		2B

Structure	Level	Pattern	Image	Note
TS19: dorsal root ganglion	Present		2C

Structure	Level	Pattern	Image	Note
TS20: cranial nerve	Present
TS20: hindlimb nerve	Present
TS20: limb nerve	Present
TS20: segmental spinal nerve	Present

Structure	Level	Pattern	Image	Note
TS21: cranial nerve	Present		2D
TS21: dermis	Present		2D
TS21: limb interdigital region mesenchyme	Present		2D
TS21: limb nerve	Present		2D
TS21: segmental spinal nerve	Present		2D
TS21: trigeminal nerve	Present		2D
TS21: forelimb muscle	Strong		2D
TS21: hindlimb muscle	Strong		2D
TS21: tail muscle	Strong		2D
TS21: back musculature	Strong	Regionally restricted	2D	Expression was detected in the paraspinal muscles.
TS21: perichondrium	Present	Regionally restricted	2D	Expression was detected in the perichondrium flanking the digit skeletal elements in both forelimbs and hindlimbs.
TS21: cartilage	Absent		2D

Structure	Level	Pattern	Image	Note
TS22: cranial nerve	Present		2E
TS22: limb interdigital region mesenchyme	Present		2E
TS22: limb nerve	Present		2E
TS22: segmental spinal nerve	Present		2E
TS22: facial muscle	Strong		2E
TS22: forelimb muscle	Strong		2E
TS22: hindlimb muscle	Strong		2E
TS22: tail muscle	Strong		2E
TS22: back musculature	Strong	Regionally restricted	2E	Expression was detected in the paraspinal muscles.
TS22: perichondrium	Present	Regionally restricted	2E	Expression was detected in the perichondrium flanking the digit skeletal elements in both forelimbs and hindlimbs.
TS22: cartilage	Absent		2E

Structure	Level	Pattern	Image	Note
TS23: limb interdigital region mesenchyme	Present		2F
TS23: perichondrium	Present	Regionally restricted	2F	Expression was detected in the perichondrium flanking the digit skeletal elements in both forelimbs and hindlimbs.
TS23: dermis	Present	Diffuse	2F

Structure	Level	Pattern	Image	Note
TS21: limb interdigital region mesenchyme	Present		2H
TS21: limb muscle	Present		2H
TS21: limb nerve	Present		2H
TS21: perichondrium	Present	Regionally restricted	2H	Expression was detected in the perichondrium flanking the digit skeletal elements in both forelimbs and hindlimbs.

Structure	Level	Pattern	Image	Note
TS22: limb interdigital region mesenchyme	Present		2I
TS22: limb muscle	Present		2I
TS22: limb nerve	Present		2I
TS22: perichondrium	Present	Regionally restricted	2I	Expression was detected in the perichondrium flanking the digit skeletal elements in both forelimbs and hindlimbs.

Structure	Level	Pattern	Image	Note
TS21: 4th ventricle choroid plexus	Strong	Regionally restricted	3A	Expression was detected in the choroid plexus epithelium.
TS21: midbrain	Present	Regionally restricted	3A	Expression was detected in the midbrain nuclei.
TS21: brain ventricular layer	Present	Regionally restricted	3A	Expression was detected in the ependyma in all ventricles of the developing brain.

Structure	Level	Pattern	Image	Note
TS28: arteriole smooth muscle	Present
TS28: choroid plexus epithelium	Present
TS28: hair arrector pili muscle	Present
TS28: tendon	Present
TS28: cerebellum Purkinje cell layer	Weak
TS28: cerebellum granule cell layer	Weak
TS28: cerebellum white matter	Weak
TS28: spinal cord	Present	Single cells
TS28: pancreatic duct	Present	Regionally restricted		Expression was detected in smooth muscle cells of pancreatic ducts.

Structure	Level	Pattern	Image	Note
TS22: head mesenchyme	Present		3B	Staining in developing craniofacial mesenchyme included the developing calvaria, base of the skull and snout region.
TS22: epidermis	Absent		3B
TS22: nasal epithelium	Absent		3B
TS22: nasal septum cartilage	Absent		3B

Structure	Level	Pattern	Image	Note
TS21: head mesenchyme	Present		3C	Staining in developing craniofacial mesenchyme included the developing calvaria, base of the skull and snout region.
TS21: oral region mesenchyme	Present	Diffuse	3C
TS21: palatal shelf	Absent		3C
TS21: tongue	Absent		3C

Structure	Level	Pattern	Image	Note
TS23: head mesenchyme	Present			Staining in developing craniofacial mesenchyme included the developing calvaria, base of the skull and snout region.

Structure	Level	Pattern	Image	Note
TS21: tongue muscle	Present	Regionally restricted	3D	Expression was detected in anterior muscle fibers in the tongue.
TS21: cranial muscle	Present		3D
TS21: tooth	Present		3D
TS21: tooth mesenchyme	Present		3D

Structure	Level	Pattern	Image	Note
TS24: dental papilla	Absent		3E
TS24: tooth epithelium	Absent		3E
TS24: jaw mesenchyme	Present	Regionally restricted	3E	Expression was detected in the mesenchyme forming the tooth socket.

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present	Regionally restricted	3F	Expression was noted in isolated regions of the thoracic spinal cord.
TS21: back musculature	Present	Regionally restricted	3F	Expression was detected in the paraspinal muscles.
TS21: dorsal root ganglion	Present		3F

Structure	Level	Pattern	Image	Note
TS22: extrinsic ocular muscle	Present
TS22: face mesenchyme	Present	Regionally restricted		Expression was noted in craniofacial mesenchyme around the developing eye.
TS22: eye	Absent

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Strong		3G
TS28: retina ganglion cell layer	Strong		3G

Structure	Level	Pattern	Image	Note
TS28: cornea endothelium	Present		3H
TS28: iris	Present		3H
TS28: cornea epithelium	Absent		3H
TS28: cornea stroma	Absent		3H

Structure	Level	Pattern	Image	Note
TS21: skeletal muscle	Present			Expression was detected in skeletal muscle myoblasts.
TS21: parietal mesothelium	Present
TS21: visceral mesothelium	Present
TS21: gut	Present	Regionally restricted		A reticular staining was noted on the surface of all segments of the gut from the stomach to the colon.

Structure	Level	Pattern	Image	Note
TS24: skeletal muscle	Present			Expression was detected in skeletal muscle myoblasts.
TS24: pancreatic acinus	Present	Regionally restricted		Expression was detected in stromal and ductal cells invading the acini.
TS24: peritoneum parietal mesothelium	Present			Expression was found in the parietal peritoneum lining the abdominal side of the diaphragm.

Structure	Level	Pattern	Image	Note
TS28: sciatic nerve	Strong		4E
TS28: hamstring muscle	Absent		4E

Structure	Level	Pattern	Image	Note
TS28: dorsal root ganglion	Strong		4F
TS28: spinal nerve	Strong	Regionally restricted	4F	Strong expression was detected in the nerve root adjacent to the dorsal root ganglion.

Structure	Level	Pattern	Image	Note
TS28: perineurium	Present		4G
TS28: sciatic nerve	Present		4G

Structure	Level	Pattern	Image	Note
TS28: perineurium	Present		4H	Expression was detected in the Schwann cells.

Structure	Level	Pattern	Image	Note
TS28: perineurium	Present		4I	Expression was detected in the Schwann cells.

Structure	Level	Pattern	Image	Note
TS24: visceral mesothelium	Present		5A
TS24: bronchus smooth muscle	Present		5A

Structure	Level	Pattern	Image	Note
TS28: bronchus smooth muscle	Present		5B
TS28: bronchus epithelium	Weak		5B	Occasional light nuclear staining was detected in bronchial epithelium.

Structure	Level	Pattern	Image	Note
TS24: arterial system smooth muscle	Present		5C

Structure	Level	Pattern	Image	Note
TS28: aorta smooth muscle	Present		5D

Structure	Level	Pattern	Image	Note
TS21: midgut	Present	Regionally restricted	5E	A stippled staining was noted on the surface of the jejunum.

Structure	Level	Pattern	Image	Note
TS21: intestine smooth muscle	Absent		5F
TS21: intestine	Present	Regionally restricted	5F	Expression was detected external to the muscularis mucosa.

Structure	Level	Pattern	Image	Note
TS24: intestine smooth muscle	Absent		5G
TS24: intestine	Present	Regionally restricted	5G	Sub-adventitial expression was maintained and didn't extend internal to the muscularis mucosa.

Structure	Level	Pattern	Image	Note
TS24: intrinsic nerve plexus	Present	Regionally restricted	5H	Staining was adjacent to the myenteric plexus (of Meissner) and the lack of staining internal to muscularis mucosa, suggests that it is not associated with the mucosal plexus of Auerbach.

Structure	Level	Pattern	Image	Note
TS28: myenteric nerve plexus	Present		5I

Structure	Level	Pattern	Image	Note
TS28: pleura	Present		5J
TS28: sympathetic ganglion	Present		5J
TS28: sympathetic nerve trunk	Present		5J

Structure	Level	Pattern	Image	Note
TS21: cap mesenchyme	Present		6A
TS21: renal tubule	Present	Regionally restricted	6A	Expression was detected in collecting tubules.
TS21: mesenchyme of metanephros	Present	Scattered	6A

Structure	Level	Pattern	Image	Note
TS24: glomerular mesangium of maturing glomerular tuft	Present		6B' , 6B
TS24: maturing glomerular tuft	Present		6B' , 6B
TS24: parietal epithelium of maturing renal corpuscle	Present		6B' , 6B
TS24: mesenchyme of metanephros	Present	Scattered	6B

Structure	Level	Pattern	Image	Note
TS28: glomerular tuft	Present		6C

Structure	Level	Pattern	Image	Note
TS28: arcuate artery	Present		6D
TS28: arteriole smooth muscle	Present		6D
TS28: glomerular tuft	Present		6D

Structure	Level	Pattern	Image	Note
TS28: glomerular mesangium of glomerular tuft	Present		6E
TS28: glomerular capillary endothelium	Absent		6E
TS28: visceral epithelium of mature renal corpuscle	Absent		6E

Structure	Level	Pattern	Image	Note
TS24: outflow tract aortic component	Present	Regionally restricted	6F	Intense mesenchymal expression was detected in the aorta and pulmonic artery roots.
TS24: outflow tract pulmonary component	Present	Regionally restricted	6F	Intense mesenchymal expression was detected in the aorta and pulmonic artery roots.
TS24: aortic valve leaflet	Present	Regionally restricted	6F	Expression was detected in aortic valve leaflet mesenchyme.

Structure	Level	Pattern	Image	Note
TS24: cardiac muscle tissue	Present	Scattered	6G
TS24: endocardial lining	Present	Scattered	6G

Structure	Level	Pattern	Image	Note
TS24: endocardial lining	Present	Regionally restricted	6H	Expression was detected in the endothelial cells of the trabecular lining.
TS24: cardiac muscle tissue	Present	Scattered	6H
TS24: pericardium	Present		6H

Structure	Level	Pattern	Image	Note
TS28: blood vessel smooth muscle	Present
TS28: interstitium of the testis	Present
TS28: tunica albuginea of testis	Present