Antibody:	anti-Zfhep
Detection system:	Secondary antibody coupled to fluorescein

Structure	Level	Pattern	Image	Note
TS19: palatal shelf mesenchyme	Present	Regionally restricted	1B	Expression in the nuclei of mesenchyme of palatal shelves. At early stages, stronger expression in layers of cells near epithelium than in mesenchyme in center of palatal shelves.
TS19: palatal shelf epithelium	Absent		1B

Structure	Level	Pattern	Image	Note
TS20: palatal shelf mesenchyme	Present	Regionally restricted	1C	Expression in the nuclei of mesenchyme of palatal shelves.
TS20: palatal shelf epithelium	Absent		1C

Structure	Level	Pattern	Image	Note
TS21: palatal shelf mesenchyme	Present	Regionally restricted	1E	Expression in the nuclei of mesenchyme of palatal shelves.
TS21: palatal shelf epithelium	Absent		1E

Structure	Level	Pattern	Image	Note
TS22: palatal shelf mesenchyme	Present	Regionally restricted	1F	Expression in fused tips of horizontal palatal shelves.
TS22: palatal shelf epithelium	Absent		1F

Structure	Level	Pattern	Image	Note
TS21: perichondrium	Present	Regionally restricted	1G	Expression in perichondrium around developing Meckel's cartilage.

Structure	Level	Pattern	Image	Note
TS22: forelimb muscle	Present	Regionally restricted	2E	Expression in nuclei in limb muscle.

Structure	Level	Pattern	Image	Note
TS21: tongue muscle	Present	Regionally restricted	2H	Strongest expression in cells forming a pattern of striations similar to developing muscles.

Structure	Level	Pattern	Image	Note
TS22: telencephalon ventricular layer	Present	Regionally restricted	3A	Expression in entire ventricular zone of lateral ventricle.
TS22: diencephalon ventricular layer	Present	Regionally restricted	3A	Expression in ventricular zone of 3rd ventricle.

Structure	Level	Pattern	Image	Note
TS21: telencephalon ventricular layer	Present	Regionally restricted	3B	Expression in ventricular zone of 3rd ventricle.

Structure	Level	Pattern	Image	Note
TS22: medulla oblongata	Strong	Regionally restricted	4A	Strong expression in cells at edge of medulla oblongata, both in the precerebellar neuroepithelium and after cells exit mitosis and migrate across medulla.

Structure	Level	Pattern	Image	Note
TS22: pons	Present	Regionally restricted	4C	Expression in cells on surface of pons, external to nerve tracts, consistent with identification as the anterior precerebellar extramural migratory stream (PEMS).
TS22: vestibulo-cochlear ganglion	Present		4C

Structure	Level	Pattern	Image	Note
TS17: neural tube	Ambiguous		7A	Little or no expression in neural tube at E10.5.

Structure	Level	Pattern	Image	Note
TS19: neural tube ventricular layer	Strong		7B	Strong expression in spinal cord ventricular zone from E11.5 through E13.5.

Structure	Level	Pattern	Image	Note
TS20: spinal cord ventricular layer	Strong		7C	Strong expression in spinal cord ventricular zone from E11.5 through E13.5.
TS20: dorsal root ganglion	Strong		7C

Structure	Level	Pattern	Image	Note
TS21: spinal cord ventricular layer	Strong		7D	Strong expression in spinal cord ventricular zone from E11.5 through E13.5.

Structure	Level	Pattern	Image	Note
TS22: spinal cord	Present	Regionally restricted	7E	Expression in isolated cells in spinal cord and in a layer around the central canal.

Structure	Level	Pattern	Image	Note
TS28: spinal cord	Present	Scattered	7F	Weak expression in cells scattered in gray and white matter.