About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:233954 Tu S, et al., Co-repressor CBFA2T2 regulates pluripotency and germline development. Nature. 2016 Jun 16;534(7607):387-90
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5811138
Gene symbol: Tfap2c
Gene name: transcription factor AP-2, gamma
Antibody: Anti-AP-2gamma (H-77; sc-8977)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Assay notes: Data in Extended Data Figure 4 (ED4)e and f were presented in graphs of the number of primordial germ cells per embryo at various developmental stages.
Results
Specimen 4h Cbfa2t2+/-: embryonic day 8.0; Cbfa2t2em1Dare/Cbfa2t2+ (more )
Note: Double labeled: green - Tfap2c; red - Ehmt1 (assay MGI:5811143). Late headfold stage.
Structure Level Pattern Image Note
TS11: primordial germ cell Present 4h Cbfa2t2+/- Merged , 4h Cbfa2t2+/- AP-2gamma
Specimen 4h Cbfa2t2-/-: embryonic day 8.0; Cbfa2t2em1Dare/Cbfa2t2em1Dare (more )
Note: Double labeled: green - Tfap2c; red - Ehmt1 (assay MGI:5811143). Late headfold stage.
Structure Level Pattern Image Note
TS11: primordial germ cell Present 4h Cbfa2t2-/- Merged , 4h Cbfa2t2-/- AP-2gamma
Specimen 4i Cbfa2t2+/+: embryonic day 8.75 (more )
Note: Double labeled: green - Tfap2c; red - H3K9me2.
Structure Level Pattern Image Note
TS13: primordial germ cell Present 4i Cbfa2t2+/+ Merged
Specimen 4i Cbfa2t2-/-: embryonic day 8.75; Cbfa2t2em1Dare/Cbfa2t2em1Dare (more )
Note: Double labeled: green - Tfap2c; red - H3K9me2.
Structure Level Pattern Image Note
TS13: primordial germ cell Present 4i Cbfa2t2-/- Merged
Specimen ED4e LS-EB +/+: embryonic day 7.0 (more )
Note: Late streak-early bud.
Structure Level Pattern Image Note
TS10: primordial germ cell Present
Specimen ED4e LS-EB +/-: embryonic day 7.0; Cbfa2t2em1Dare/Cbfa2t2+ (more )
Note: Late streak-early bud.
Structure Level Pattern Image Note
TS10: primordial germ cell Present
Specimen ED4e LS-EB -/-: embryonic day 7.0; Cbfa2t2em1Dare/Cbfa2t2em1Dare (more )
Note: Late streak-early bud.
Structure Level Pattern Image Note
TS10: primordial germ cell Present
Specimen ED4e MB-LB +/+: embryonic day 7.25 (more )
Note: Mid bud-late bud.
Structure Level Pattern Image Note
TS11: primordial germ cell Present
Specimen ED4e MB-LB +/-: embryonic day 7.25; Cbfa2t2em1Dare/Cbfa2t2+ (more )
Note: Mid bud-late bud.
Structure Level Pattern Image Note
TS11: primordial germ cell Present
Specimen ED4e MB-LB -/-: embryonic day 7.25; Cbfa2t2em1Dare/Cbfa2t2em1Dare (more )
Note: Mid bud-late bud.
Structure Level Pattern Image Note
TS11: primordial germ cell Present
Specimen ED4e EHF-2st. +/+: embryonic day 7.5-8.0 (more )
Note: Early headfold-2 somites.
Structure Level Pattern Image Note
TS12: primordial germ cell Present
Specimen ED4e EHF-2st. +/-: embryonic day 7.5-8.0; Cbfa2t2em1Dare/Cbfa2t2+ (more )
Note: Early headfold-2 somites.
Structure Level Pattern Image Note
TS12: primordial germ cell Present
Specimen ED4e EHF-2st. -/-: embryonic day 7.5-8.0; Cbfa2t2em1Dare/Cbfa2t2em1Dare (more )
Note: Early headfold-2 somites.
Structure Level Pattern Image Note
TS12: primordial germ cell Present
Specimen ED4f LS +/+: embryonic day 7.0 (more )
Note: Late streak.
Structure Level Pattern Image Note
TS10: primordial germ cell Present
Specimen ED4f LS +/-: embryonic day 7.0; Cbfa2t2em1Dare/Cbfa2t2+ (more )
Note: Late streak.
Structure Level Pattern Image Note
TS10: primordial germ cell Present
Specimen ED4f 0B +/+: embryonic day 7.0 (more )
Note: 0 bud.
Structure Level Pattern Image Note
TS10: primordial germ cell Present
Specimen ED4f 0B +/-: embryonic day 7.0; Cbfa2t2em1Dare/Cbfa2t2+ (more )
Note: 0 bud.
Structure Level Pattern Image Note
TS10: primordial germ cell Present
Specimen ED4f 0B -/-: embryonic day 7.0; Cbfa2t2em1Dare/Cbfa2t2em1Dare (more )
Note: 0 bud.
Structure Level Pattern Image Note
TS10: primordial germ cell Present
Specimen ED4f EB +/+: embryonic day 7.0 (more )
Note: Early bud.
Structure Level Pattern Image Note
TS10: primordial germ cell Present
Specimen ED4f EB +/-: embryonic day 7.0; Cbfa2t2em1Dare/Cbfa2t2+ (more )
Note: Early bud.
Structure Level Pattern Image Note
TS10: primordial germ cell Present
Specimen ED4f EB -/-: embryonic day 7.0; Cbfa2t2em1Dare/Cbfa2t2em1Dare (more )
Note: Early bud.
Structure Level Pattern Image Note
TS10: primordial germ cell Present
Specimen ED4f MB +/+: embryonic day 7.25 (more )
Note: Mid bud.
Structure Level Pattern Image Note
TS11: primordial germ cell Present
Specimen ED4f MB +/-: embryonic day 7.25; Cbfa2t2em1Dare/Cbfa2t2+ (more )
Note: Mid bud.
Structure Level Pattern Image Note
TS11: primordial germ cell Present
Specimen ED4f MB -/-: embryonic day 7.25; Cbfa2t2em1Dare/Cbfa2t2em1Dare (more )
Note: Mid bud.
Structure Level Pattern Image Note
TS11: primordial germ cell Present
Specimen ED4f LB +/+: embryonic day 7.5 (more )
Note: Late bud.
Structure Level Pattern Image Note
TS11: primordial germ cell Present
Specimen ED4f LB +/-: embryonic day 7.5; Cbfa2t2em1Dare/Cbfa2t2+ (more )
Note: Late bud.
Structure Level Pattern Image Note
TS11: primordial germ cell Present
Specimen ED4f LB -/-: embryonic day 7.5; Cbfa2t2em1Dare/Cbfa2t2em1Dare (more )
Note: Late bud.
Structure Level Pattern Image Note
TS11: primordial germ cell Present
Specimen ED4f EHF +/+: embryonic day 7.5 (more )
Note: Early headfold.
Structure Level Pattern Image Note
TS11: primordial germ cell Present
Specimen ED4f EHF -/-: embryonic day 7.5; Cbfa2t2em1Dare/Cbfa2t2em1Dare (more )
Note: Early headfold.
Structure Level Pattern Image Note
TS11: primordial germ cell Present
Specimen ED4f LHF +/+: embryonic day 8.0 (more )
Note: Late headfold.
Structure Level Pattern Image Note
TS11: primordial germ cell Present
Specimen ED4f LHF +/-: embryonic day 8.0; Cbfa2t2em1Dare/Cbfa2t2+ (more )
Note: Late headfold.
Structure Level Pattern Image Note
TS11: primordial germ cell Present
Specimen ED4f LHF -/-: embryonic day 8.0; Cbfa2t2em1Dare/Cbfa2t2em1Dare (more )
Note: Late headfold.
Structure Level Pattern Image Note
TS11: primordial germ cell Present
Specimen ED4f 2St. +/+: embryonic day 8.0 (more )
Note: 2 somites.
Structure Level Pattern Image Note
TS12: primordial germ cell Present
Specimen ED4f 2St. +/-: embryonic day 8.0; Cbfa2t2em1Dare/Cbfa2t2+ (more )
Note: 2 somites.
Structure Level Pattern Image Note
TS12: primordial germ cell Present
Specimen ED4f 2St. -/-: embryonic day 8.0; Cbfa2t2em1Dare/Cbfa2t2em1Dare (more )
Note: 2 somites.
Structure Level Pattern Image Note
TS12: primordial germ cell Present
Specimen ED4g +/+: embryonic day 7.25 (more )
Note: Double labeled: green - Tfap2c; red - Sox2 (assay MGI:5811256). Mid-bud stage.
Structure Level Pattern Image Note
TS11: primordial germ cell Present ED4g +/+ Merged , ED4g +/+ AP2gamma
Specimen ED4g -/-: embryonic day 7.25; Cbfa2t2em1Dare/Cbfa2t2em1Dare (more )
Note: Double labeled: green - Tfap2c; red - Sox2 (assay MGI:5811256). Mid-bud stage.
Structure Level Pattern Image Note
TS11: primordial germ cell Present ED4g -/- Merged , ED4g -/- AP2gamma
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
04/23/2024
MGI 6.23 		The Jackson Laboratory