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Gene Expression Data
Assay Details
Assay
Reference: J:233954 Tu S, et al., Co-repressor CBFA2T2 regulates pluripotency and germline development. Nature. 2016 Jun 16;534(7607):387-90
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5811138
Gene symbol: Tfap2c
Gene name: transcription factor AP-2, gamma
Antibody: Anti-AP-2gamma (H-77; sc-8977)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Assay notes: Data in Extended Data Figure 4 (ED4)e and f were presented in graphs of the number of primordial germ cells per embryo at various developmental stages.
Results
Specimen 4h Cbfa2t2+/-: embryonic day 8.0; Cbfa2t2em1Dare/Cbfa2t2+ (more )
Note: Double labeled: green - Tfap2c; red - Ehmt1 (assay MGI:5811143). Late headfold stage.
Structure Level Pattern Image Note
TS11: primordial germ cell Present 4h Cbfa2t2+/- Merged , 4h Cbfa2t2+/- AP-2gamma
Specimen 4h Cbfa2t2-/-: embryonic day 8.0; Cbfa2t2em1Dare/Cbfa2t2em1Dare (more )
Note: Double labeled: green - Tfap2c; red - Ehmt1 (assay MGI:5811143). Late headfold stage.
Structure Level Pattern Image Note
TS11: primordial germ cell Present 4h Cbfa2t2-/- Merged , 4h Cbfa2t2-/- AP-2gamma
Specimen 4i Cbfa2t2+/+: embryonic day 8.75 (more )
Note: Double labeled: green - Tfap2c; red - H3K9me2.
Structure Level Pattern Image Note
TS13: primordial germ cell Present 4i Cbfa2t2+/+ Merged
Specimen 4i Cbfa2t2-/-: embryonic day 8.75; Cbfa2t2em1Dare/Cbfa2t2em1Dare (more )
Note: Double labeled: green - Tfap2c; red - H3K9me2.
Structure Level Pattern Image Note
TS13: primordial germ cell Present 4i Cbfa2t2-/- Merged
Specimen ED4e LS-EB +/+: embryonic day 7.0 (more )
Note: Late streak-early bud.
Structure Level Pattern Image Note
TS10: primordial germ cell Present
Specimen ED4e LS-EB +/-: embryonic day 7.0; Cbfa2t2em1Dare/Cbfa2t2+ (more )
Note: Late streak-early bud.
Structure Level Pattern Image Note
TS10: primordial germ cell Present
Specimen ED4e LS-EB -/-: embryonic day 7.0; Cbfa2t2em1Dare/Cbfa2t2em1Dare (more )
Note: Late streak-early bud.
Structure Level Pattern Image Note
TS10: primordial germ cell Present
Specimen ED4e MB-LB +/+: embryonic day 7.25 (more )
Note: Mid bud-late bud.
Structure Level Pattern Image Note
TS11: primordial germ cell Present
Specimen ED4e MB-LB +/-: embryonic day 7.25; Cbfa2t2em1Dare/Cbfa2t2+ (more )
Note: Mid bud-late bud.
Structure Level Pattern Image Note
TS11: primordial germ cell Present
Specimen ED4e MB-LB -/-: embryonic day 7.25; Cbfa2t2em1Dare/Cbfa2t2em1Dare (more )
Note: Mid bud-late bud.
Structure Level Pattern Image Note
TS11: primordial germ cell Present
Specimen ED4e EHF-2st. +/+: embryonic day 7.5-8.0 (more )
Note: Early headfold-2 somites.
Structure Level Pattern Image Note
TS12: primordial germ cell Present
Specimen ED4e EHF-2st. +/-: embryonic day 7.5-8.0; Cbfa2t2em1Dare/Cbfa2t2+ (more )
Note: Early headfold-2 somites.
Structure Level Pattern Image Note
TS12: primordial germ cell Present
Specimen ED4e EHF-2st. -/-: embryonic day 7.5-8.0; Cbfa2t2em1Dare/Cbfa2t2em1Dare (more )
Note: Early headfold-2 somites.
Structure Level Pattern Image Note
TS12: primordial germ cell Present
Specimen ED4f LS +/+: embryonic day 7.0 (more )
Note: Late streak.
Structure Level Pattern Image Note
TS10: primordial germ cell Present
Specimen ED4f LS +/-: embryonic day 7.0; Cbfa2t2em1Dare/Cbfa2t2+ (more )
Note: Late streak.
Structure Level Pattern Image Note
TS10: primordial germ cell Present
Specimen ED4f 0B +/+: embryonic day 7.0 (more )
Note: 0 bud.
Structure Level Pattern Image Note
TS10: primordial germ cell Present
Specimen ED4f 0B +/-: embryonic day 7.0; Cbfa2t2em1Dare/Cbfa2t2+ (more )
Note: 0 bud.
Structure Level Pattern Image Note
TS10: primordial germ cell Present
Specimen ED4f 0B -/-: embryonic day 7.0; Cbfa2t2em1Dare/Cbfa2t2em1Dare (more )
Note: 0 bud.
Structure Level Pattern Image Note
TS10: primordial germ cell Present
Specimen ED4f EB +/+: embryonic day 7.0 (more )
Note: Early bud.
Structure Level Pattern Image Note
TS10: primordial germ cell Present
Specimen ED4f EB +/-: embryonic day 7.0; Cbfa2t2em1Dare/Cbfa2t2+ (more )
Note: Early bud.
Structure Level Pattern Image Note
TS10: primordial germ cell Present
Specimen ED4f EB -/-: embryonic day 7.0; Cbfa2t2em1Dare/Cbfa2t2em1Dare (more )
Note: Early bud.
Structure Level Pattern Image Note
TS10: primordial germ cell Present
Specimen ED4f MB +/+: embryonic day 7.25 (more )
Note: Mid bud.
Structure Level Pattern Image Note
TS11: primordial germ cell Present
Specimen ED4f MB +/-: embryonic day 7.25; Cbfa2t2em1Dare/Cbfa2t2+ (more )
Note: Mid bud.
Structure Level Pattern Image Note
TS11: primordial germ cell Present
Specimen ED4f MB -/-: embryonic day 7.25; Cbfa2t2em1Dare/Cbfa2t2em1Dare (more )
Note: Mid bud.
Structure Level Pattern Image Note
TS11: primordial germ cell Present
Specimen ED4f LB +/+: embryonic day 7.5 (more )
Note: Late bud.
Structure Level Pattern Image Note
TS11: primordial germ cell Present
Specimen ED4f LB +/-: embryonic day 7.5; Cbfa2t2em1Dare/Cbfa2t2+ (more )
Note: Late bud.
Structure Level Pattern Image Note
TS11: primordial germ cell Present
Specimen ED4f LB -/-: embryonic day 7.5; Cbfa2t2em1Dare/Cbfa2t2em1Dare (more )
Note: Late bud.
Structure Level Pattern Image Note
TS11: primordial germ cell Present
Specimen ED4f EHF +/+: embryonic day 7.5 (more )
Note: Early headfold.
Structure Level Pattern Image Note
TS11: primordial germ cell Present
Specimen ED4f EHF -/-: embryonic day 7.5; Cbfa2t2em1Dare/Cbfa2t2em1Dare (more )
Note: Early headfold.
Structure Level Pattern Image Note
TS11: primordial germ cell Present
Specimen ED4f LHF +/+: embryonic day 8.0 (more )
Note: Late headfold.
Structure Level Pattern Image Note
TS11: primordial germ cell Present
Specimen ED4f LHF +/-: embryonic day 8.0; Cbfa2t2em1Dare/Cbfa2t2+ (more )
Note: Late headfold.
Structure Level Pattern Image Note
TS11: primordial germ cell Present
Specimen ED4f LHF -/-: embryonic day 8.0; Cbfa2t2em1Dare/Cbfa2t2em1Dare (more )
Note: Late headfold.
Structure Level Pattern Image Note
TS11: primordial germ cell Present
Specimen ED4f 2St. +/+: embryonic day 8.0 (more )
Note: 2 somites.
Structure Level Pattern Image Note
TS12: primordial germ cell Present
Specimen ED4f 2St. +/-: embryonic day 8.0; Cbfa2t2em1Dare/Cbfa2t2+ (more )
Note: 2 somites.
Structure Level Pattern Image Note
TS12: primordial germ cell Present
Specimen ED4f 2St. -/-: embryonic day 8.0; Cbfa2t2em1Dare/Cbfa2t2em1Dare (more )
Note: 2 somites.
Structure Level Pattern Image Note
TS12: primordial germ cell Present
Specimen ED4g +/+: embryonic day 7.25 (more )
Note: Double labeled: green - Tfap2c; red - Sox2 (assay MGI:5811256). Mid-bud stage.
Structure Level Pattern Image Note
TS11: primordial germ cell Present ED4g +/+ Merged , ED4g +/+ AP2gamma
Specimen ED4g -/-: embryonic day 7.25; Cbfa2t2em1Dare/Cbfa2t2em1Dare (more )
Note: Double labeled: green - Tfap2c; red - Sox2 (assay MGI:5811256). Mid-bud stage.
Structure Level Pattern Image Note
TS11: primordial germ cell Present ED4g -/- Merged , ED4g -/- AP2gamma
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory