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Gene Expression Data
Assay Details
Assay
Reference: J:169043 Chapman G, et al., Notch inhibition by the ligand Delta-Like 3 defines the mechanism of abnormal vertebral segmentation in spondylocostal dysostosis. Hum Mol Genet. 2011 Mar 1;20(5):905-16
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5790075
Gene symbol: Notch1
Gene name: notch 1
Antibody: anti-cleaved Notch1 (Val1744)
Detection system: Secondary antibody
Results
Specimen 3K: embryonic day 9.5 (more )
Note: Inset is a higher magnification of the boxed region. Triple labeled: blue - cleaved Notch1; green - Notch1 (assay MGI:5790067); red - Dll3 (assay MGI:5790068).
Structure Level Pattern Image Note
TS15: tail unsegmented mesenchyme Present Regionally restricted 3K cleaved Notch1 , 3K merge Expression is in the rostral presomitic mesoderm.
Specimen 4A: embryonic day 9.5 (more )
Note: Double labeled: red - cleaved Notch1; green - Notch1 (assay MGI:5790067).
Structure Level Pattern Image Note
TS15: tail unsegmented mesenchyme Present Regionally restricted 4A Expression is in thin stripes of cells in the rostral presomitic mesoderm.
Specimen 4B: embryonic day 9.5 (more )
Note: Double labeled: red - cleaved Notch1; green - Notch1 (assay MGI:5790067).
Structure Level Pattern Image Note
TS15: tail unsegmented mesenchyme Present Regionally restricted 4B Expression is in thin stripes of cells in the rostral presomitic mesoderm.
Specimen 4C: embryonic day 9.5; Dll3tm1Rbe/Dll3tm1Rbe (more )
Note: Double labeled: red - cleaved Notch1; green - Notch1 (assay MGI:5790067).
Structure Level Pattern Image Note
TS15: tail unsegmented mesenchyme Present Regionally restricted 4C In this mutant an expression domain in the rostral presomitic mesoderm is missing and there is one broad rostral band of expression.
Specimen 4D: embryonic day 9.5; Dll3tm1Rbe/Dll3tm1Rbe (more )
Note: Double labeled: red - cleaved Notch1; green - Notch1 (assay MGI:5790067).
Structure Level Pattern Image Note
TS15: tail unsegmented mesenchyme Present Regionally restricted 4D In this mutant an expression domain in the rostral presomitic mesoderm is missing and there is one broad rostral band of expression.
Specimen 4E: embryonic day 9.5 (more )
Note: (G and H) are higher magnifications of (E). Triple labeled: red - cleaved Notch1; green - Dll3 (assay MGI:5790068); blue - Dll1 (assay MGI:5790062).
Structure Level Pattern Image Note
TS15: tail unsegmented mesenchyme Present Regionally restricted 4E merge , 4E cleaved Notch1 , 4G merge , 4H , 4G cleaved Notch1 Expression is in the rostral presomitic mesoderm.
Specimen 4I: embryonic day 9.5; Dll3tm1Rbe/Dll3tm1Rbe (more )
Note: (J) is a higher magnification of (I). Triple labeled: red - cleaved Notch1; green - Dll3 (assay MGI:5790068); blue - Dll1 (assay MGI:5790062).
Structure Level Pattern Image Note
TS15: tail unsegmented mesenchyme Present Regionally restricted 4J merge , 4J cleaved Notch1 , 4I merge , 4I cleaved Notch1 In this mutant an expression domain in the rostral presomitic mesoderm is missing and there is one broad rostral band of expression.
Specimen S3a: embryonic day 9.5 (more )
Note: Triple labeled: red - cleaved Notch1; green - Dll3 (assay MGI:5790068); blue - Dll1 (assay MGI:5790062).
Structure Level Pattern Image Note
TS15: tail unsegmented mesenchyme Present Regionally restricted S3a merge , S3a cleaved Notch1 Expression is in thin stripes of cells in the rostral presomitic mesoderm.
Specimen S3b: embryonic day 9.5 (more )
Note: Triple labeled: red - cleaved Notch1; green - Dll3 (assay MGI:5790068); blue - Dll1 (assay MGI:5790062).
Structure Level Pattern Image Note
TS15: tail unsegmented mesenchyme Present Regionally restricted S3b merge , S3b cleaved Notch1 Expression is in thin stripes of cells in the rostral presomitic mesoderm.
Specimen S3c: embryonic day 11.5 (more )
Note: Triple labeled: red - cleaved Notch1; green - Dll3 (assay MGI:5790068); blue - Dll1 (assay MGI:5790062).
Structure Level Pattern Image Note
TS19: tail unsegmented mesenchyme Present Regionally restricted S3c merge , S3c cleaved Notch1 Expression is in thin stripes of cells in the rostral presomitic mesoderm.
Specimen S3d: embryonic day 11.5 (more )
Note: Triple labeled: red - cleaved Notch1; green - Dll3 (assay MGI:5790068); blue - Dll1 (assay MGI:5790062).
Structure Level Pattern Image Note
TS19: tail unsegmented mesenchyme Present Regionally restricted S3d merge , S3d cleaved Notch1 Expression is in thin stripes of cells in the rostral presomitic mesoderm.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/28/2024
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