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Gene Expression Data
Assay Details
Assay
Reference: J:169043 Chapman G, et al., Notch inhibition by the ligand Delta-Like 3 defines the mechanism of abnormal vertebral segmentation in spondylocostal dysostosis. Hum Mol Genet. 2011 Mar 1;20(5):905-16
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5790067
Gene symbol: Notch1
Gene name: notch 1
Antibody: anti-Notch1
Detection system: Secondary antibody
Results
Specimen 2E: embryonic day 9.5 (more )
Note: Double labeled: green - Notch1; red - Golga2 (assay MGI:5790065).
Structure Level Pattern Image Note
TS15: tail unsegmented mesenchyme Present Regionally restricted 2E Notch1 , 2E merge Expression is in the rostral presomitic mesoderm.
Specimen 2H: embryonic day 9.5 (more )
Note: Double labeled: green - Notch1; red - Lamp1 (assay MGI:5790070).
Structure Level Pattern Image Note
TS15: tail unsegmented mesenchyme Present Regionally restricted 2H merge , 2H Notch1 Expression is in the rostral presomitic mesoderm.
Specimen 2I: embryonic day 9.5 (more )
Note: Triple labeled: green - Notch1; red - Lamp1 (assay MGI:5790070); blue - Dll3 (assay MGI:5790068).
Structure Level Pattern Image Note
TS15: tail unsegmented mesenchyme Present Regionally restricted 2I merge , 2I Notch1 Expression is in the rostral presomitic mesoderm.
Specimen 3E: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: tail unsegmented mesenchyme Present Regionally restricted 3E Expression is in the presomitic mesoderm.
TS15: somite Present Regionally restricted 3E Expression is highest in the forming and nascent somite.
Specimen 3G: embryonic day 9.5 (more )
Note: Double labeled: green - Notch1; red - Dll3 (assay MGI:5790068).
Structure Level Pattern Image Note
TS15: tail unsegmented mesenchyme Present Regionally restricted 3G merge , 3G Notch1 Expression is in the rostral presomitic mesoderm.
TS15: somite Present Regionally restricted 3G Notch1 , 3G merge Expression is in the forming somite.
Specimen 3H: embryonic day 9.5 (more )
Note: (I) is a higher magnification of (H), inset is a higher magnification of the boxed region. Double labeled: red - Notch1; green - Dll1 (assay MGI:5790062).
Structure Level Pattern Image Note
TS15: tail unsegmented mesenchyme Present Regionally restricted 3H Notch1 , 3I merge , 3I Notch1 , 3H merge Expression is in the rostral presomitic mesoderm.
TS15: somite Present Regionally restricted 3H Notch1 , 3H merge , 3I merge , 3I Notch1 Expression is in the forming somite.
Specimen 3J: embryonic day 9.5 (more )
Note: Triple labeled: red - Notch1; green - Dll1 (assay MGI:5790062); blue - Dll3 (assay MGI:5790068).
Structure Level Pattern Image Note
TS15: tail unsegmented mesenchyme Present Regionally restricted 3J Expression is in the rostral presomitic mesoderm.
Specimen 3K: embryonic day 9.5 (more )
Note: Inset is a higher magnification of the boxed region. Triple labeled: green - Notch1; red - Dll3 (assay MGI:5790068); blue - cleaved Notch1 (assay MGI:5790075).
Structure Level Pattern Image Note
TS15: tail unsegmented mesenchyme Present Regionally restricted 3K Notch1 , 3K merge Expression is in the rostral presomitic mesoderm.
Specimen 3L: embryonic day 9.5 (more )
Note: Inset is a higher magnification of the boxed region. Triple labeled: red - Notch1; blue - Dll3 (assay MGI:5790068); green - Golga2 (assay MGI:5790065).
Structure Level Pattern Image Note
TS15: tail unsegmented mesenchyme Present Regionally restricted 3L Notch1 , 3L merge Expression is in the rostral presomitic mesoderm.
Specimen 4A: embryonic day 9.5 (more )
Note: Double labeled: green - Notch1; red - cleaved Notch1 (assay MGI:5790075).
Structure Level Pattern Image Note
TS15: tail unsegmented mesenchyme Present Regionally restricted 4A Expression is in the rostral presomitic mesoderm.
Specimen 4B: embryonic day 9.5 (more )
Note: Double labeled: green - Notch1; red - cleaved Notch1 (assay MGI:5790075).
Structure Level Pattern Image Note
TS15: tail unsegmented mesenchyme Present Regionally restricted 4B Expression is in the rostral presomitic mesoderm.
Specimen 4C: embryonic day 9.5; Dll3tm1Rbe/Dll3tm1Rbe (more )
Note: Double labeled: green - Notch1; red - cleaved Notch1 (assay MGI:5790075).
Structure Level Pattern Image Note
TS15: tail unsegmented mesenchyme Present Regionally restricted 4C Expression is in the rostral presomitic mesoderm.
Specimen 4D: embryonic day 9.5; Dll3tm1Rbe/Dll3tm1Rbe (more )
Note: Double labeled: green - Notch1; red - cleaved Notch1 (assay MGI:5790075).
Structure Level Pattern Image Note
TS15: tail unsegmented mesenchyme Present Regionally restricted 4D Expression is in the rostral presomitic mesoderm.
Specimen S2: embryonic day 9.5 (more )
Note: Image analysis of Figure 2I. Triple labeled: green - Notch1; red - Lamp1 (assay MGI:5790070); blue - Dll3 (assay MGI:5790068).
Structure Level Pattern Image Note
TS15: tail unsegmented mesenchyme Present Regionally restricted S2f , S2g , S2h , S2i Expression is in the rostral presomitic mesoderm.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory