Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:226410
Jurek B, et al., Oxytocin Regulates Stress-Induced Crf Gene Transcription through CREB-Regulated Transcription Coactivator 3. J Neurosci. 2015 Sep 2;35(35):12248-60
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:5762576
|
|
Gene symbol:
|
Crh
|
|
Gene name:
|
corticotropin releasing hormone
|
|
Probe:
|
Crf cDNA
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
|
Results
|
Specimen
5A:
(close  )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6J
|
|
Mutant Allele(s):
|
Oxtrtm2.1Knis/Oxtr+
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Male
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
5D:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6J
|
|
Mutant Allele(s):
|
Oxtrtm2.1Knis/Oxtr+
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Male
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: amygdala |
Ambiguous |
Single cells |
5D
|
The authors discussed a lack of double labeling, implying there is little or no expression.
|
Specimen
5G/I:
embryonic day 18.5; Oxtrtm2.1Knis/Oxtr +
(more  )
Note: Double labeled: red - Crh; green - Oxtr (assay MGI:5762578).
Specimen
5G/I:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6J
|
|
Mutant Allele(s):
|
Oxtrtm2.1Knis/Oxtr+
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: red - Crh; green - Oxtr (assay MGI:5762578).
|
|
Analysis Tools