Gene Expression Data
Assay Details
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Assay
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Reference:
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J:203647
Imamura F, et al., Pax6 regulates Tbr1 and Tbr2 expressions in olfactory bulb mitral cells. Mol Cell Neurosci. 2013 May;54:58-70
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5759947
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Gene symbol:
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Pax6
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Gene name:
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paired box 6
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Antibody:
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anti PAX6 (DSHB)
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Detection system:
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Secondary antibody coupled to Alexa Fluor 555
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Assay notes:
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The authors did not specify which antibody was used, this or another Pax6 antibody (MGI:5759939).
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Results
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Specimen
1D:
embryonic day 11.5
(more  )
Note: Double labeled with BrdU (green).
Specimen
1D:
(close  )
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Genetic Background:
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CD-1
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Age:
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embryonic day 11.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled with BrdU (green).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: telencephalon |
Present |
Regionally restricted |
1D
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Expression is in mitral cell precursors in the ventricular zone of the olfactory bulb.
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Specimen
1E:
embryonic day 13.5
(more )
Note: Double labeled with BrdU (green).
Specimen
1E:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled with BrdU (green).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: main olfactory bulb |
Present |
Regionally restricted |
1E
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Expression is in the ventricular zone with a few expressing cells are in the intermediate zone.
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Specimen
1F:
embryonic day 15.5
(more )
Note: Double labeled with BrdU (green).
Specimen
1F:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled with BrdU (green).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: main olfactory bulb |
Present |
Regionally restricted |
1F
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Expression is in the ventricular zone with some expressing cells in the intermediate zone.
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Specimen
2A:
embryonic day 11.5
(more )
Note: Double labeled: red - Pax6; green - Fabp7 (assay MGI:5759952).
Specimen
2A:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 11.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Pax6; green - Fabp7 (assay MGI:5759952).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: telencephalon |
Present |
Regionally restricted |
2A
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Expression is in the olfactory bulb in the ventricular zone.
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Specimen
2B:
embryonic day 13.5
(more )
Note: Double labeled: red - Pax6; green - Tbr1 (assay MGI:5759950). (C) is a higher magnification.
Specimen
2B:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Pax6; green - Tbr1 (assay MGI:5759950). (C) is a higher magnification.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: main olfactory bulb |
Present |
Regionally restricted |
2C1
,
2C3
,
2B
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2C
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Expression is in the ventricular zone.
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Specimen
2D:
embryonic day 13.5
(more )
Note: Double labeled: red - Pax6; green - Eomes (Tbr2; assay MGI:5759951). (E) is a higher magnification.
Specimen
2D:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Pax6; green - Eomes (Tbr2; assay MGI:5759951). (E) is a higher magnification.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: main olfactory bulb |
Present |
Regionally restricted |
2E1
,
2D
,
2E3
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2E
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Expression is in the ventricular zone.
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Specimen
7A:
embryonic day 17.5
(more )
Note: Double labeled with BrdU (green).
Specimen
7A:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 17.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled with BrdU (green).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: main olfactory bulb |
Present |
Regionally restricted |
7A
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Expression is in the glomerular, mitral cell layer, and superficial granule cell layers.
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