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Gene Expression Data
Assay Details
Assay
Reference: J:149474 Schwarz Q, et al., Neuropilin-mediated neural crest cell guidance is essential to organise sensory neurons into segmented dorsal root ganglia. Development. 2009 Jun;136(11):1785-9
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5752034
Gene symbol: Ngfr
Gene name: nerve growth factor receptor (TNFR superfamily, member 16)
Antibody: anti-p75
Detection system: Secondary antibody
Results
Specimen 1M/N/P: embryonic day 9.5 (more )
Note: Double labeled: green - Ngfr; red - Nrp2 (assay MGI:5752030).
Structure Level Pattern Image Note
TS15: dorsal root ganglion Present 1M , 1N , 1P
TS15: trunk mesenchyme derived from neural crest Present Regionally restricted 1M Expressed in sympathetic anlagen at the dorsal aorta.
Specimen 1Q/R/T: embryonic day 9.5 (more )
Note: Double labeled: green - Ngfr; red - Nrp2 (assay MGI:5752032).
Structure Level Pattern Image Note
TS15: dorsal root ganglion Present 1T , 1Q , 1R
TS15: trunk mesenchyme derived from neural crest Present Regionally restricted 1Q Expressed in sympathetic anlagen at the dorsal aorta.
Specimen 2A/C/D/F: embryonic day 9.5; Sema3atm1.1Tyag/Sema3atm1.1Tyag (more )
Note: Double labeled: green - Ngfr; red - Nrp2 (assay MGI:5752032).
Structure Level Pattern Image Note
TS15: sclerotome Present Regionally restricted 2F , 2A , 2C , 2D Expressed in anterior sclerotome.
Specimen 2G/I/J/L: embryonic day 9.5; Sema3ftm1.2Ddg/Sema3ftm1.2Ddg (more )
Note: Double labeled: green - Ngfr; red - Nrp2 (assay MGI:5752032).
Structure Level Pattern Image Note
TS15: sclerotome Present Widespread 2J , 2I , 2L , 2G Expressed in both anterior and posterior sclerotome. Ectopic neural crest cells in the posterior sclerotome were Nrp2-positive.
Specimen 2M/O/P/R: embryonic day 9.5; Sema3atm1.1Tyag/Sema3atm1.1Tyag (more )
Note: Double labeled: green - Ngfr; red - Nrp2 (assay MGI:5752032).
Structure Level Pattern Image Note
TS15: sclerotome Present Regionally restricted 2M , 2O , 2R , 2P Expressed in anterior sclerotome.
TS15: trunk mesenchyme derived from neural crest Present Regionally restricted 2P , 2R , 2M , 2O Few neural crest cells migrated into the anterior sclerotome, as most neural crest cells were rerouted into the intersomitic/perisomitic space. Ectopic neural crest cells in the posterior sclerotome were Nrp2-negative.
Specimen 2S/U/V/X: embryonic day 9.5; Sema3atm1.1Tyag/Sema3atm1.1Tyag (more )
Note: Double labeled: green - Ngfr; red - Nrp2 (assay MGI:5752030).
Structure Level Pattern Image Note
TS15: sclerotome Present Regionally restricted 2U , 2X , 2V , 2S Expressed in anterior sclerotome.
TS15: trunk mesenchyme derived from neural crest Present Regionally restricted 2X , 2V , 2S , 2U Few neural crest cells migrated into the anterior sclerotome, as most neural crest cells were rerouted into the intersomitic/perisomitic space. Ectopic neural crest cells in the posterior sclerotome were Nrp1-positive.
Specimen 3A: embryonic day 9.5; Nrp1tm1Ddg/Nrp1+, Nrp2tm1Ddg/Nrp2+ (more )
Note: Double labeled: green - Ngfr; red - Emcn (assay MGI:5752035).
Structure Level Pattern Image Note
TS15: sclerotome Present Regionally restricted 3A Most neural crest cells migrated in the anterior sclerotome.
TS15: trunk mesenchyme derived from neural crest Present Regionally restricted 3A Few neural crest cells migrated in the intersomitic/perisomitic space.
Specimen 3B: embryonic day 9.5; Nrp1tm1Ddg/Nrp1tm1Ddg, Nrp2tm1Ddg/Nrp2tm1Ddg (more )
Note: Double labeled: green - Ngfr; red - Emcn (assay MGI:5752035).
Structure Level Pattern Image Note
TS15: sclerotome Present 3B Neural crest cells invaded both anterior and posterior sclerotome.
TS15: trunk mesenchyme derived from neural crest Present Regionally restricted 3B Many neural crest cells migrated in the intersomitic/perisomitic space.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
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