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Gene Expression Data
Assay Details
Assay
Reference: J:205268 Ahmad N, et al., Pitx3 directly regulates Foxe3 during early lens development. Int J Dev Biol. 2013;57(9-10):741-51
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5689326
Gene symbol: Prox1
Gene name: prospero homeobox 1
Antibody: Anti-Prox1 (AB5475)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 4m/s: embryonic day 10.5 (more )
Note: Double labeled: green - Prox1; red - Pitx3 (assay MGI:5689308).
Structure Level Pattern Image Note
TS17: lens pit Present Single cells 4s , 4m Very few cells are labeled at E10.5. There was almost complete overlap of expression with Pitx3.
Specimen 4n/t: embryonic day 10.5; Pitx3ak/Pitx3ak (more )
Note: Double labeled: green - Prox1; red - Pitx3 (assay MGI:5689308).
Structure Level Pattern Image Note
TS17: lens pit Absent 4t , 4n
Specimen 4o/u: embryonic day 11.5 (more )
Note: Double labeled: green - Prox1; red - Pitx3 (assay MGI:5689308).
Structure Level Pattern Image Note
TS19: lens vesicle Present 4u , 4o More cells are labeled at E11.5. There was almost complete overlap of expression with Pitx3.
Specimen 4p/v: embryonic day 11.5; Pitx3ak/Pitx3ak (more )
Note: Double labeled: green - Prox1; red - Pitx3 (assay MGI:5689308).
Structure Level Pattern Image Note
TS19: lens vesicle Absent 4v , 4p
Specimen 4q/w: embryonic day 12.5 (more )
Note: Double labeled: green - Prox1; red - Pitx3 (assay MGI:5689308).
Structure Level Pattern Image Note
TS20: lens vesicle Present 4w , 4q The whole lens is labeled at E12.5. There was almost complete overlap of expression with Pitx3.
Specimen 4r/x: embryonic day 12.5; Pitx3ak/Pitx3ak (more )
Note: Double labeled: green - Prox1; red - Pitx3 (assay MGI:5689308).
Structure Level Pattern Image Note
TS20: lens vesicle Absent 4x , 4r
Specimen 5e/i: embryonic day 11.5 (more )
Note: Triple labeled: green - Prox1; red - Pitx3 (assay MGI:5689308); gray - BrdU.
Structure Level Pattern Image Note
TS19: lens vesicle Present 5e , 5i
Specimen 5f/j: embryonic day 11.5; Pitx3ak/Pitx3ak (more )
Note: Triple labeled: green - Prox1; red - Pitx3 (assay MGI:5689308); gray - BrdU.
Structure Level Pattern Image Note
TS19: lens vesicle Absent 5f , 5j
Specimen S1c/d: embryonic day 11.5; Foxe3tm1Pca/Foxe3tm1Pca (more )
Structure Level Pattern Image Note
TS19: lens vesicle Present Regionally restricted S1c/d Expression was more anterior in the mutant lens compared to wild type. Expression completely overlaps with Pitx3.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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