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Gene Expression Data
Assay Details
Assay
Reference: J:205268 Ahmad N, et al., Pitx3 directly regulates Foxe3 during early lens development. Int J Dev Biol. 2013;57(9-10):741-51
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5689308
Gene symbol: Pitx3
Gene name: paired-like homeodomain transcription factor 3
Antibody: Anti-Pitx3 (N-20) sc-19307X
Detection system: Secondary antibody coupled to Cy3
Results
Specimen 1b/d: embryonic day 10.5 (more )
Note: Double labeled: red - Pitx3; green - Pax6 (assay MGI:5689309).
Structure Level Pattern Image Note
TS17: lens pit Present 1b , 1d

Specimen 1f/h: embryonic day 10.5; Pitx3ak/Pitx3ak (more )
Note: Double labeled: red - Pitx3; green - Pax6 (assay MGI:5689309).
Structure Level Pattern Image Note
TS17: lens pit Absent 1f , 1h

Specimen 1j/l: embryonic day 11.5 (more )
Note: Double labeled: red - Pitx3; green - Pax6 (assay MGI:5689309).
Structure Level Pattern Image Note
TS19: lens vesicle Present 1j , 1l

Specimen 1n/p: embryonic day 11.5; Pitx3ak/Pitx3ak (more )
Note: Double labeled: red - Pitx3; green - Pax6 (assay MGI:5689309).
Structure Level Pattern Image Note
TS19: lens vesicle Ambiguous 1p , 1n

Specimen 3g/s: embryonic day 10.5 (more )
Note: Double labeled: red - Pitx3; green - Foxe3 (assay MGI:5689319).
Structure Level Pattern Image Note
TS17: lens pit Present 3s , 3g There was almost complete overlap of expression with Foxe3.

Specimen 3h/t: embryonic day 10.5; Pitx3ak/Pitx3ak (more )
Note: Double labeled: red - Pitx3; green - Foxe3 (assay MGI:5689319).
Structure Level Pattern Image Note
TS17: lens pit Absent 3t , 3h

Specimen 3i/u: embryonic day 11.5 (more )
Note: Double labeled: red - Pitx3; green - Foxe3 (assay MGI:5689319).
Structure Level Pattern Image Note
TS19: lens vesicle Present 3u , 3i There was almost complete overlap of expression with Foxe3.

Specimen 3j/v: embryonic day 11.5; Pitx3ak/Pitx3ak (more )
Note: Double labeled: red - Pitx3; green - Foxe3 (assay MGI:5689319).
Structure Level Pattern Image Note
TS19: lens vesicle Ambiguous 3j , 3v

Specimen 3k/w: embryonic day 12.5 (more )
Note: Double labeled: red - Pitx3; green - Foxe3 (assay MGI:5689319).
Structure Level Pattern Image Note
TS20: lens vesicle Present 3k , 3w There was almost complete overlap of expression with Foxe3.

Specimen 3l/x: embryonic day 12.5; Pitx3ak/Pitx3ak (more )
Note: Double labeled: red - Pitx3; green - Foxe3 (assay MGI:5689319).
Structure Level Pattern Image Note
TS20: lens vesicle Ambiguous 3x , 3l

Specimen 4g/s: embryonic day 10.5 (more )
Note: Double labeled: red - Pitx3; green - Prox1 (assay MGI:5689326).
Structure Level Pattern Image Note
TS17: lens pit Present 4s , 4g There was almost complete overlap of expression with Prox1.

Specimen 4h/t: embryonic day 10.5; Pitx3ak/Pitx3ak (more )
Note: Double labeled: red - Pitx3; green - Prox1 (assay MGI:5689326).
Structure Level Pattern Image Note
TS17: lens pit Absent 4t , 4h

Specimen 4i/u: embryonic day 11.5 (more )
Note: Double labeled: red - Pitx3; green - Prox1 (assay MGI:5689326).
Structure Level Pattern Image Note
TS19: lens vesicle Present 4i , 4u There was almost complete overlap of expression with Prox1.

Specimen 4j/v: embryonic day 11.5; Pitx3ak/Pitx3ak (more )
Note: Double labeled: red - Pitx3; green - Prox1 (assay MGI:5689326).
Structure Level Pattern Image Note
TS19: lens vesicle Ambiguous 4j , 4v

Specimen 4k/w: embryonic day 12.5 (more )
Note: Double labeled: red - Pitx3; green - Prox1 (assay MGI:5689326).
Structure Level Pattern Image Note
TS20: lens vesicle Present 4k , 4w There was almost complete overlap of expression with Prox1.

Specimen 4l/x: embryonic day 12.5; Pitx3ak/Pitx3ak (more )
Note: Double labeled: red - Pitx3; green - Prox1 (assay MGI:5689326).
Structure Level Pattern Image Note
TS20: lens vesicle Ambiguous 4l , 4x

Specimen 5g/i: embryonic day 11.5 (more )
Note: Triple labeled: red - Pitx3; green - Prox1 (assay MGI:5689326); gray - BrdU.
Structure Level Pattern Image Note
TS19: lens vesicle epithelium Present Regionally restricted 5i , 5g Expression was detected throughout the lens epithelium.

Specimen 5h/j: embryonic day 11.5; Pitx3ak/Pitx3ak (more )
Note: Triple labeled: red - Pitx3; green - Prox1 (assay MGI:5689326); gray - BrdU.
Structure Level Pattern Image Note
TS19: lens vesicle epithelium Ambiguous 5h , 5j

Specimen 6g/s: embryonic day 10.5 (more )
Note: Double labeled: red - Pitx3; green - Cdh1 (assay MGI:5689345).
Structure Level Pattern Image Note
TS17: lens pit Present 6s , 6g

Specimen 6h/t: embryonic day 10.5; Pitx3ak/Pitx3ak (more )
Note: Double labeled: red - Pitx3; green - Cdh1 (assay MGI:5689345).
Structure Level Pattern Image Note
TS17: lens pit Absent 6h , 6t

Specimen 6i/u: embryonic day 11.5 (more )
Note: Double labeled: red - Pitx3; green - Cdh1 (assay MGI:5689345).
Structure Level Pattern Image Note
TS19: lens vesicle Present 6i , 6u

Specimen 6j/v: embryonic day 11.5; Pitx3ak/Pitx3ak (more )
Note: Double labeled: red - Pitx3; green - Cdh1 (assay MGI:5689345).
Structure Level Pattern Image Note
TS19: lens vesicle Ambiguous 6v , 6j

Specimen 6k/w: embryonic day 12.5 (more )
Note: Double labeled: red - Pitx3; green - Cdh1 (assay MGI:5689345).
Structure Level Pattern Image Note
TS20: lens vesicle Present 6k , 6w

Specimen 6l/x: embryonic day 12.5; Pitx3ak/Pitx3ak (more )
Note: Double labeled: red - Pitx3; green - Cdh1 (assay MGI:5689345).
Structure Level Pattern Image Note
TS20: lens vesicle Ambiguous 6x , 6l

Specimen S1a/b: embryonic day 11.5; Foxe3tm1Pca/Foxe3tm1Pca (more )
Structure Level Pattern Image Note
TS19: lens vesicle Present S1a/b Expression completely overlaps with Prox1.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/21/2024
MGI 6.23
The Jackson Laboratory